PMID- 35007923
OWN - NLM
STAT- MEDLINE
DCOM- 20220215
LR  - 20220215
IS  - 1879-0909 (Electronic)
IS  - 0141-0229 (Linking)
VI  - 155
DP  - 2022 Apr
TI  - Biocatalysis of heterogenously-expressed d-lactonohydrolases and its efficient 
      preparation of desirable d-pantoic acid.
PG  - 109981
LID - S0141-0229(21)00239-8 [pii]
LID - 10.1016/j.enzmictec.2021.109981 [doi]
AB  - d-Pantoic acid (D-PA) is an essential intermediate for the production of 
      d-pantolactone. Here, three d-lactonohydrolases (D-Lacs), namely, Fm-Lac from 
      Fusarium moniliforme SW-902, Fp-Lac from Fusarium proliferatum Nirenberg ECU2002, 
      and Fo-Lac from Fusarium oxysporum AKU3702 were heterogeneously expressed in 
      Pichia pastoris. The constructed recombinant strains produced D-Lacs of 
      1263 U/mL, 1025 U/mL, and 948 U/mL in a 3-L fermenter, respectively. 
      Simultaneously, these three D-Lacs were used to resolve racemic pantolactone 
      (DL-PL), the hydrolysis rate by Fo-Lac over 40% and the enantiomeric excesses was 
      99% after 4 h reaction, which outperformed Fm-Lac and Fp-Lac. Under the 800 mL 
      scale reaction, the hydrolysis rate of DL-PL reached 39.2% with a D-PA 
      concentration of 144.6 g/L and space-time yield of 36.2 g/L/h correspondingly. 
      This is the highest catalytic efficiency reported so far, which shows that D-Lac 
      heterologously expressed by P. pastoris has excellent industrial application 
      prospects.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Sun, Ruobin
AU  - Sun R
AD  - The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of 
      Biotechnology, Jiangnan University, Wuxi 214122, China.
FAU - Zheng, Pu
AU  - Zheng P
AD  - The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of 
      Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address: 
      zhengpu@jiangan.edu.cn.
FAU - Wu, Dan
AU  - Wu D
AD  - The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of 
      Biotechnology, Jiangnan University, Wuxi 214122, China.
FAU - Chen, Pengcheng
AU  - Chen P
AD  - The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of 
      Biotechnology, Jiangnan University, Wuxi 214122, China.
FAU - Bai, Yanbing
AU  - Bai Y
AD  - Hangzhou Xinfu Technology Co., Ltd. Hangzhou 311301, China.
FAU - Wang, Jun
AU  - Wang J
AD  - Hangzhou Xinfu Technology Co., Ltd. Hangzhou 311301, China.
LA  - eng
PT  - Journal Article
DEP - 20220105
PL  - United States
TA  - Enzyme Microb Technol
JT  - Enzyme and microbial technology
JID - 8003761
RN  - 0 (Hydroxybutyrates)
RN  - 0 (Recombinant Proteins)
RN  - 0J1TL6G6J9 (pantoic acid)
SB  - IM
MH  - Biocatalysis
MH  - Hydrolysis
MH  - Hydroxybutyrates
MH  - *Pichia/genetics/metabolism
MH  - Recombinant Proteins/genetics/metabolism
OTO - NOTNLM
OT  - D-Lactonohydrolase
OT  - D-Pantoic acid
OT  - Enzymatic resolution
OT  - Heterologous expression
OT  - Pichia pastoris
EDAT- 2022/01/11 06:00
MHDA- 2022/02/16 06:00
CRDT- 2022/01/10 20:29
PHST- 2021/10/13 00:00 [received]
PHST- 2021/12/22 00:00 [revised]
PHST- 2021/12/28 00:00 [accepted]
PHST- 2022/01/11 06:00 [pubmed]
PHST- 2022/02/16 06:00 [medline]
PHST- 2022/01/10 20:29 [entrez]
AID - S0141-0229(21)00239-8 [pii]
AID - 10.1016/j.enzmictec.2021.109981 [doi]
PST - ppublish
SO  - Enzyme Microb Technol. 2022 Apr;155:109981. doi: 10.1016/j.enzmictec.2021.109981. 
      Epub 2022 Jan 5.