PMID- 35008862
OWN - NLM
STAT- MEDLINE
DCOM- 20220203
LR  - 20220203
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 1
DP  - 2021 Dec 31
TI  - Anti-Inflammatory, Antioxidant, Moisturizing, and Antimelanogenesis Effects of 
      Quercetin 3-O-beta-D-Glucuronide in Human Keratinocytes and Melanoma Cells via 
      Activation of NF-kappaB and AP-1 Pathways.
LID - 10.3390/ijms23010433 [doi]
LID - 433
AB  - Quercetin 3-O-beta-D-glucuronide (Q-3-G), the glucuronide conjugate of quercetin, 
      has been reported as having anti-inflammatory properties in the 
      lipopolysaccharide-stimulated macrophages, as well as anticancer and antioxidant 
      properties. Unlike quercetin, which has been extensively described to possess a 
      wide range of pharmacological activities including skin protective effects, the 
      pharmacological benefits and mechanisms Q-3-G in the skin remained to be 
      elucidated. This study focused on characterizing the skin protective properties, 
      including anti-inflammatory and antioxidant properties, of Q-3-G against 
      UVB-induced or H(2)O(2)-induced oxidative stress, the hydration effects, and 
      antimelanogenesis activities using human keratinocytes (HaCaT) and melanoma 
      (B16F10) cells. Q-3-G down-regulated the expression of the pro-inflammatory gene 
      and cytokine such as cyclooxygenase-2 (COX-2) and tumor necrosis factor (TNF)-alpha 
      in H(2)O(2) or UVB-irradiated HaCaT cells. We also showed that Q-3-G exhibits an 
      antioxidant effect using free radical scavenging assays, flow cytometry, and an 
      increased expression of nuclear factor erythroid 2- related factor 2 (Nrf2). 
      Q-3-G reduced melanin production in alpha-melanocyte-stimulating hormone 
      (alpha-MSH)-induced B16F10 cells. The hydration effects and mechanisms of Q-3-G were 
      examined by evaluating the moisturizing factor-related genes, such as 
      transglutaminase-1 (TGM-1), filaggrin (FLG), and hyaluronic acid synthase 
      (HAS)-1. In addition, Q-3-G increased the phosphorylation of c-Jun, Jun 
      N-terminal kinase (JNK), Mitogen-activated protein kinase (MAPK) kinase 4 (MKK4), 
      and TAK1, involved in the MAPKs/AP-1 pathway, and the phosphorylation of IkappaBalpha, 
      IkappaB kinase (IKK)-alpha, Akt, and Src, involved in the NF-kappaB pathway. Taken together, 
      we have demonstrated that Q-3-G exerts anti-inflammatory, antioxidant, 
      moisturizing, and antimelanogenesis properties in human keratinocytes and 
      melanoma cells through NF-kappaB and AP-1 pathways.
FAU - Ha, Anh Thu
AU  - Ha AT
AD  - Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, 
      Korea.
FAU - Rahmawati, Laily
AU  - Rahmawati L
AUID- ORCID: 0000-0002-7977-1814
AD  - Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, 
      Korea.
FAU - You, Long
AU  - You L
AUID- ORCID: 0000-0001-6978-0066
AD  - Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, 
      Korea.
FAU - Hossain, Mohammad Amjad
AU  - Hossain MA
AUID- ORCID: 0000-0001-8941-1065
AD  - Department of Veterinary Physiology, College of Medicine, Chonbuk National 
      University, Iksan 54596, Korea.
FAU - Kim, Jong-Hoon
AU  - Kim JH
AD  - Department of Veterinary Physiology, College of Medicine, Chonbuk National 
      University, Iksan 54596, Korea.
FAU - Cho, Jae Youl
AU  - Cho JY
AUID- ORCID: 0000-0001-8141-9927
AD  - Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, 
      Korea.
AD  - Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, 
      Suwon 16419, Korea.
LA  - eng
GR  - 20008861/the Ministry of Trade, Industry, and Energy of Korea/
PT  - Journal Article
DEP - 20211231
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Melanins)
RN  - 0 (NF-kappa B)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (quercetin 3-O-glucuronide)
RN  - 9IKM0I5T1E (Quercetin)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Antioxidants/*pharmacology
MH  - HEK293 Cells
MH  - HaCaT Cells
MH  - Humans
MH  - Hydrogen Peroxide/toxicity
MH  - Keratinocytes/drug effects/*pathology/radiation effects
MH  - Melanins/*metabolism
MH  - Melanoma, Experimental/*pathology
MH  - Models, Biological
MH  - NF-kappa B/*metabolism
MH  - Quercetin/*analogs & derivatives/chemistry/pharmacology
MH  - Signal Transduction/drug effects/radiation effects
MH  - Transcription Factor AP-1/*metabolism
MH  - Transcriptional Activation/drug effects/genetics/radiation effects
MH  - Ultraviolet Rays
PMC - PMC8745180
OTO - NOTNLM
OT  - AP-1
OT  - NF-kappaB
OT  - UV protection
OT  - antimelanogenesis
OT  - moisturizing
OT  - quercetin 3-O-beta-D-glucuronide
COIS- The authors declare no conflict of interest.
EDAT- 2022/01/12 06:00
MHDA- 2022/02/04 06:00
PMCR- 2021/12/31
CRDT- 2022/01/11 01:04
PHST- 2021/12/16 00:00 [received]
PHST- 2021/12/26 00:00 [revised]
PHST- 2021/12/29 00:00 [accepted]
PHST- 2022/01/11 01:04 [entrez]
PHST- 2022/01/12 06:00 [pubmed]
PHST- 2022/02/04 06:00 [medline]
PHST- 2021/12/31 00:00 [pmc-release]
AID - ijms23010433 [pii]
AID - ijms-23-00433 [pii]
AID - 10.3390/ijms23010433 [doi]
PST - epublish
SO  - Int J Mol Sci. 2021 Dec 31;23(1):433. doi: 10.3390/ijms23010433.