PMID- 3500941
OWN - NLM
STAT- MEDLINE
DCOM- 19880127
LR  - 20171213
IS  - 8750-7587 (Print)
IS  - 0161-7567 (Linking)
VI  - 63
IP  - 5
DP  - 1987 Nov
TI  - Augmentation of lung antineutrophil elastase capacity with recombinant human 
      alpha-1-antitrypsin.
PG  - 2015-23
AB  - To evaluate the potential use of recombinant DNA-produced alpha-1-antitrypsin 
      (alpha-1-AT) to augment the lung antineutrophil elastase defenses in alpha-1-AT 
      deficiency, we compared the kinetics of intravenously administered recombinant 
      produced alpha-1-AT (r alpha-1-AT) and purified normal human plasma alpha-1-AT (p 
      alpha-1-AT) in the blood and lung of rhesus monkeys. The r alpha-1-AT was 
      produced in yeast transformed with an expressing plasmid containing a full-length 
      human alpha-1-AT complementary deoxyribonucleic acid and purified to greater than 
      99% homogeneity. The r alpha-1-AT has a molecular weight of 45,000, no 
      carbohydrates, and is identical in sequence to normal plasma alpha-1-AT except 
      for an additional N-terminal acetylmethionine. Despite its lack of carbohydrates, 
      the r alpha-1-AT inhibited human neutrophil elastase with an association rate 
      constant similar to that of p alpha-1-AT. Rhesus monkeys were infused 
      intravenously with 120 mg/kg of r alpha-1-AT (n = 13) or p alpha-1-AT (n = 12) 
      and the serum, urine, and lung epithelial lining fluid (ELF) concentrations of 
      these molecules quantified at various intervals.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Casolaro, M A
AU  - Casolaro MA
AD  - Pulmonary Branch, National Heart, Lung, and Blood Institute, Bethesda, Maryland 
      20892.
FAU - Fells, G
AU  - Fells G
FAU - Wewers, M
AU  - Wewers M
FAU - Pierce, J E
AU  - Pierce JE
FAU - Ogushi, F
AU  - Ogushi F
FAU - Hubbard, R
AU  - Hubbard R
FAU - Sellers, S
AU  - Sellers S
FAU - Forstrom, J
AU  - Forstrom J
FAU - Lyons, D
AU  - Lyons D
FAU - Kawasaki, G
AU  - Kawasaki G
AU  - et al.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Appl Physiol (1985)
JT  - Journal of applied physiology (Bethesda, Md. : 1985)
JID - 8502536
RN  - 0 (Blood Proteins)
RN  - 0 (Protease Inhibitors)
RN  - 0 (alpha 1-Antitrypsin)
SB  - IM
MH  - Animals
MH  - Blood Proteins/*metabolism
MH  - Bronchoalveolar Lavage Fluid/*metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Epithelium/metabolism
MH  - Half-Life
MH  - Humans
MH  - Lung/metabolism
MH  - Macaca mulatta
MH  - Molecular Weight
MH  - Protease Inhibitors/*metabolism
MH  - alpha 1-Antitrypsin/*pharmacokinetics
EDAT- 1987/11/01 00:00
MHDA- 1987/11/01 00:01
CRDT- 1987/11/01 00:00
PHST- 1987/11/01 00:00 [pubmed]
PHST- 1987/11/01 00:01 [medline]
PHST- 1987/11/01 00:00 [entrez]
AID - 10.1152/jappl.1987.63.5.2015 [doi]
PST - ppublish
SO  - J Appl Physiol (1985). 1987 Nov;63(5):2015-23. doi: 10.1152/jappl.1987.63.5.2015.