PMID- 35015143
OWN - NLM
STAT- MEDLINE
DCOM- 20220119
LR  - 20220119
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 106
IP  - 2
DP  - 2022 Jan
TI  - Proteomic analysis of hexahydro-beta-acids/hydroxypropyl-beta-cyclodextrin inhibit 
      Listeria monocytogenes.
PG  - 755-771
LID - 10.1007/s00253-022-11764-x [doi]
AB  - Food safety affected by food-borne pathogen has received increasing attention by 
      researchers. Listeria monocytogenes (L. monocytogenes), widespread in the 
      environment, could easily cause some diseases. The aim of this study was to 
      investigate how L. monocytogenes ATCC 19,115 regulated and shaped its proteome in 
      response to hexahydro-beta-acids (HBA) formed inclusion complex with 
      hydroxypropyl-beta-cyclodextrin (HP-beta-CD), compared to untreated cells growing under 
      optimal conditions. HP-beta-CD enhanced the solubility of HBA to 0.589 g/100 mL. The 
      minimum inhibitory concentration (MIC) and the minimal bactericidal concentration 
      (MBC) of HBA/HP-beta-CD to L. monocytogenes were 25 mug/mL and 100 mug/mL, 
      respectively. Scanning electron microscope (SEM) images demonstrated that HBA 
      could destroy the cell membrane of L. monocytogenes. The proteomic analysis 
      revealed that 2882 proteins were initially identified, among which 153 and 201 
      proteins were differentially upregulated and downregulated respectively. Changes 
      of L. monocytogenes proteome in response to treatments were mainly related to 
      carbohydrate metabolism, protein synthesis, ribosome composition proteins, cell 
      wall composition proteins, and cell division anomalies process. This research is 
      conducive to understanding the molecular mechanisms underlying the inhibiting 
      effects of HBA/HP-beta-CD against L. monocytogenes, providing novel insights for 
      further development of HBA/HP-beta-CD antimicrobial agents. KEY POINTS: * MIC and 
      MBC of HBA/HP-beta-CD to L. monocytogenes were 25 mug/mL and 100 mug/mL. * HBA/HP-beta-CD 
      cause significant changes in bacterial proteome. * The process of ribosome 
      composition and carbohydrate metabolism was inhibited.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Tian, Bingren
AU  - Tian B
AUID- ORCID: 0000-0001-9535-129X
AD  - School of Chemical Engineering and Technology, Xinjiang University, Urumqi, 
      830046, China.
FAU - Xu, Dan
AU  - Xu D
AD  - College of Chemistry, Xinjiang University, Urumqi, 830046, China.
FAU - Li, Wanrong
AU  - Li W
AD  - College of Chemistry, Xinjiang University, Urumqi, 830046, China.
FAU - Wang, Jie
AU  - Wang J
AD  - College of Chemistry, Xinjiang University, Urumqi, 830046, China.
FAU - Cheng, Jianhua
AU  - Cheng J
AD  - College of Chemistry, Xinjiang University, Urumqi, 830046, China.
FAU - Liu, Yumei
AU  - Liu Y
AD  - School of Chemical Engineering and Technology, Xinjiang University, Urumqi, 
      830046, China. xjdxlym@163.com.
LA  - eng
GR  - No. 31660490/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20220111
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 1I96OHX6EK (2-Hydroxypropyl-beta-cyclodextrin)
SB  - IM
MH  - 2-Hydroxypropyl-beta-cyclodextrin
MH  - *Listeria monocytogenes
MH  - Microbial Sensitivity Tests
MH  - Proteomics
MH  - Solubility
OTO - NOTNLM
OT  - Cyclodextrin
OT  - Hexahydro-beta-acids
OT  - Listeria monocytogenes
OT  - Mechanism
OT  - Proteomic
EDAT- 2022/01/12 06:00
MHDA- 2022/01/20 06:00
CRDT- 2022/01/11 12:28
PHST- 2021/12/07 00:00 [received]
PHST- 2022/01/05 00:00 [accepted]
PHST- 2021/12/28 00:00 [revised]
PHST- 2022/01/12 06:00 [pubmed]
PHST- 2022/01/20 06:00 [medline]
PHST- 2022/01/11 12:28 [entrez]
AID - 10.1007/s00253-022-11764-x [pii]
AID - 10.1007/s00253-022-11764-x [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2022 Jan;106(2):755-771. doi: 
      10.1007/s00253-022-11764-x. Epub 2022 Jan 11.