PMID- 35016383 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220112 IS - 2576-6422 (Electronic) IS - 2576-6422 (Linking) VI - 1 IP - 2 DP - 2018 Aug 20 TI - Processing, Characterization, and in Vivo Evaluation of Poly(l-lactic acid)-Fish Gelatin Electrospun Membranes for Biomedical Applications. PG - 226-236 LID - 10.1021/acsabm.8b00023 [doi] AB - The development of biomaterials for application in advanced therapies requires thorough characterization of its biological behavior, which ultimately entails in vivo compatibility and performance assays. Electrospun fiber membranes of poly(l-lactic acid) (PLLA) and fish gelatin blends were produced and characterized, coupling the biomechanical features of PLLA with gelatin (GEL) biocompatibility. Fiber diameter was not affected by polymer blending, whereas the swelling degree increased with increasing GEL contents for values up to 566 +/- 13%, behaving as a superhydrophilic material. Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) adhesion was favored in the PLLA-GEL membranes, and cell viability was not affected after 7 days in culture. Membranes were then evaluated for in vivo biocompatibility through subcutaneous implantation in a rat model, for up to 15 days. No significant differences between the biological behavior of PLLA, PLLA-GEL, and GEL electrospun membranes at 15 days postimplantation were verified, with attained inflammation scores supporting an acceptable tissue response, deeming them fit for further biological assays. This work demonstrates that fiber blends of PLLA and GEL present promising in vitro and in vivo characteristics to be explored for tissue engineering. FAU - Branco, Mariana AU - Branco M AD - Faculdade de Engenharia, U. Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal. FAU - Caseiro, Ana R AU - Caseiro AR AD - Departamento de Clinicas Veterinarias, Instituto de Ciencias Biomedicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, n degrees 228, 4050-313 Porto, Portugal. AD - Centro de Estudos de Ciencia Animal (CECA), Instituto de Ciencias, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal. AD - REQUIMTE/LAQV - Universidade do Porto, Departamento de Engenharia Metalurgica e Materiais, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal. FAU - Silva, Dina M AU - Silva DM AUID- ORCID: 0000-0003-3970-1632 FAU - Amorim, Irina AU - Amorim I AD - Departamento de Patologia e Imunologia Molecular, Instituto de Ciencias Biomedicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, n degrees 228, 4050-313 Porto, Portugal. AD - i3S - Instituto de Investigacao e Inovacao em Saude, Universidade do Porto, R. Alfredo Allen, 4200-135 Porto, Portugal. FAU - Rema, Alexandra AU - Rema A AD - Departamento de Patologia e Imunologia Molecular, Instituto de Ciencias Biomedicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, n degrees 228, 4050-313 Porto, Portugal. FAU - Pedrosa, Silvia S AU - Pedrosa SS AD - Departamento de Clinicas Veterinarias, Instituto de Ciencias Biomedicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, n degrees 228, 4050-313 Porto, Portugal. AD - Centro de Estudos de Ciencia Animal (CECA), Instituto de Ciencias, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal. FAU - Branquinho, Mariana V AU - Branquinho MV AD - Departamento de Clinicas Veterinarias, Instituto de Ciencias Biomedicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, n degrees 228, 4050-313 Porto, Portugal. AD - Centro de Estudos de Ciencia Animal (CECA), Instituto de Ciencias, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal. FAU - Gomes, Pedro S AU - Gomes PS AD - REQUIMTE/LAQV - Universidade do Porto, Departamento de Engenharia Metalurgica e Materiais, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal. AD - Faculdade de Medicina Dentaria, Universidade Do Porto (FMDUP), 4200-393 Porto, Portugal. FAU - Fernandes, Maria H AU - Fernandes MH AD - REQUIMTE/LAQV - Universidade do Porto, Departamento de Engenharia Metalurgica e Materiais, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal. AD - Faculdade de Medicina Dentaria, Universidade Do Porto (FMDUP), 4200-393 Porto, Portugal. FAU - Santos, Jose D AU - Santos JD AD - Faculdade de Engenharia, U. Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal. AD - REQUIMTE/LAQV - Universidade do Porto, Departamento de Engenharia Metalurgica e Materiais, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal. FAU - Mauricio, Ana C AU - Mauricio AC AUID- ORCID: 0000-0002-0018-9363 AD - Departamento de Clinicas Veterinarias, Instituto de Ciencias Biomedicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, n degrees 228, 4050-313 Porto, Portugal. AD - Centro de Estudos de Ciencia Animal (CECA), Instituto de Ciencias, Tecnologias e Agroambiente da Universidade do Porto (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal. FAU - Sencadas, Vitor AU - Sencadas V AUID- ORCID: 0000-0003-1986-1348 LA - eng PT - Journal Article DEP - 20180806 PL - United States TA - ACS Appl Bio Mater JT - ACS applied bio materials JID - 101729147 SB - IM OTO - NOTNLM OT - biocompatibility OT - biomimetic extracellular matrix OT - electrospun membranes OT - poly(l-lactic acid)-fish gelatin blends OT - wettability EDAT- 2018/08/20 00:00 MHDA- 2018/08/20 00:01 CRDT- 2022/01/12 01:00 PHST- 2022/01/12 01:00 [entrez] PHST- 2018/08/20 00:00 [pubmed] PHST- 2018/08/20 00:01 [medline] AID - 10.1021/acsabm.8b00023 [doi] PST - ppublish SO - ACS Appl Bio Mater. 2018 Aug 20;1(2):226-236. doi: 10.1021/acsabm.8b00023. Epub 2018 Aug 6.