PMID- 35019374 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220210 IS - 2576-6422 (Electronic) IS - 2576-6422 (Linking) VI - 3 IP - 10 DP - 2020 Oct 19 TI - Supreme Catalytic Properties of Enzyme Nanoparticles Based on Ferritin Self-Assembly. PG - 7158-7167 LID - 10.1021/acsabm.0c00961 [doi] AB - In this study, we aimed to address if enzymes self-assembled on the biological nanosphere matrix would have some nanoscale effects and then improve the catalytic ability and other characteristics especially for the resistance to adverse conditions. Initially, we evaluated two different forms of ferritins, DNA-binding protein from starved cells (Dps) and recombinant heavy chain of human ferritin (rHF), fused with methyl parathion hydrolase (MPH) to be self-assembled as the dodecamer and 24-mer enzyme nanoparticles (enzyme-NPs), Dps-MPH and MPH-rHF. Both self-assembled enzyme-NPs showed superior catalytic activity and k(cat) drastically increased, but MPH-rHF showed better performance than Dps-MPH. Next, Escherichia coli alkaline phosphatase (EAP) and the canonical mutant EAP(D101S) were chosen to confirm if rHF-based self-assembly generally improves the catalysis of enzymes with the different catalytic abilities. Additionally, restoration of the enzyme native forms on the rHF shell by increasing the flexibility of the polylinker between the catalytic units and the nanoscaffold further improved the catalytic activities of enzyme-NPs. Some of the nanoparticles exhibited better residual activities under extreme conditions. Conclusively, the enzyme-NPs based on rHF self-assembly provided an effective strategy for enzyme engineering. FAU - Chen, Qingmei AU - Chen Q AD - Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, China. AD - Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, Guangdong 510642, China. AD - Key Laboratory of Zoonosis of Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou, Guangdong 510642, China. FAU - Men, Dong AU - Men D AUID- ORCID: 0000-0003-1190-9570 AD - State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China. FAU - Sun, Tianyu AU - Sun T AD - Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, China. FAU - Zhang, Yan AU - Zhang Y AD - Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, China. FAU - Yuan, Qianqian AU - Yuan Q AD - Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, China. FAU - Hu, Taidou AU - Hu T AD - Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, China. FAU - Hu, Zhangsheng AU - Hu Z AD - Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, China. FAU - Wu, Jun AU - Wu J AD - Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, China. AD - Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, Guangdong 510642, China. AD - Key Laboratory of Zoonosis of Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou, Guangdong 510642, China. FAU - Deng, Yiqun AU - Deng Y AD - Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, China. AD - Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, Guangdong 510642, China. AD - Key Laboratory of Zoonosis of Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou, Guangdong 510642, China. FAU - Zhang, Xian-En AU - Zhang XE AUID- ORCID: 0000-0003-1347-3168 AD - National Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China. FAU - Wen, Jikai AU - Wen J AUID- ORCID: 0000-0002-4010-4416 AD - Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, China. AD - Guangdong Laboratory for Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, Guangdong 510642, China. AD - Key Laboratory of Zoonosis of Ministry of Agriculture and Rural Affairs, South China Agricultural University, Guangzhou, Guangdong 510642, China. LA - eng PT - Journal Article DEP - 20200923 PL - United States TA - ACS Appl Bio Mater JT - ACS applied bio materials JID - 101729147 SB - IM OTO - NOTNLM OT - catalytic activity OT - enzyme nanoparticle OT - nanocarrier OT - recombinant heavy chain of human ferritin OT - self-assembly EDAT- 2020/10/19 00:00 MHDA- 2020/10/19 00:01 CRDT- 2022/01/12 12:17 PHST- 2022/01/12 12:17 [entrez] PHST- 2020/10/19 00:00 [pubmed] PHST- 2020/10/19 00:01 [medline] AID - 10.1021/acsabm.0c00961 [doi] PST - ppublish SO - ACS Appl Bio Mater. 2020 Oct 19;3(10):7158-7167. doi: 10.1021/acsabm.0c00961. Epub 2020 Sep 23.