PMID- 35020796
OWN - NLM
STAT- MEDLINE
DCOM- 20220311
LR  - 20230302
IS  - 1938-3207 (Electronic)
IS  - 0002-9165 (Print)
IS  - 0002-9165 (Linking)
VI  - 115
IP  - 3
DP  - 2022 Mar 4
TI  - Effect of a single high dose of vitamin D3 on cytokines, chemokines, and growth 
      factor in patients with moderate to severe COVID-19.
PG  - 790-798
LID - 10.1093/ajcn/nqab426 [doi]
AB  - BACKGROUND: The modulating effect of vitamin D on cytokine concentrations in 
      severe coronavirus disease 2019 (COVID-19) remains unknown. OBJECTIVES: We aimed 
      to investigate the effect of a single high dose of vitamin D3 on cytokines, 
      chemokines, and growth factor in hospitalized patients with moderate to severe 
      COVID-19. METHODS: This is a post hoc, ancillary, and exploratory analysis from a 
      multicenter, double-blind, placebo-controlled, randomized clinical trial. 
      Patients with moderate to severe COVID-19 were recruited from 2 hospitals in Sao 
      Paulo, Brazil. Of 240 randomly assigned patients, 200 were assessed in this study 
      and randomly assigned to receive a single oral dose of 200,000 IU vitamin D3 
      (n = 101) or placebo (n = 99). The primary outcome was hospital length of stay, 
      which has been published in our previous study. The prespecified secondary 
      outcomes were serum concentrations of IL-1beta, IL-6, IL-10, TNF-alpha, and 
      25-hydroxyvitamin D. The post hoc exploratory secondary outcomes were IL-4, 
      IL-12p70, IL-17A, IFN-gamma, granulocyte-macrophage colony-stimulating factor 
      (GM-CSF), IL-8, IFN-inducible protein-10 (IP-10), macrophage inflammatory 
      protein-1beta (MIP-1beta), monocyte chemoattractant protein-1 (MCP-1), vascular 
      endothelial growth factor (VEGF), and leukocyte count. Generalized estimating 
      equations for repeated measures, with Bonferroni's adjustment, were used for 
      testing all outcomes. RESULTS: The study included 200 patients with a mean +/- SD 
      age of 55.5 +/- 14.3 y and BMI of 32.2 +/- 7.1 kg/m2, of which 109 (54.5%) were male. 
      GM-CSF concentrations showed a significant group-by-time interaction effect (P = 
      0.04), although the between-group difference at postintervention after 
      Bonferroni's adjustment was not significant. No significant effects were observed 
      for the other outcomes. CONCLUSIONS: The findings do not support the use of a 
      single dose of 200,000 IU vitamin D3, compared with placebo, for the improvement 
      of cytokines, chemokines, and growth factor in hospitalized patients with 
      moderate to severe COVID-19.This trial was registered at clinicaltrials.gov as 
      NCT04449718.
CI  - (c) The Author(s) 2022. Published by Oxford University Press on behalf of the 
      American Society for Nutrition.
FAU - Fernandes, Alan L
AU  - Fernandes AL
AUID- ORCID: 0000-0002-6786-3922
AD  - Rheumatology Division, Hospital das Clinicas da Faculdade de Medicina da 
      Universidade de Sao Paulo (HCFMUSP), Faculdade de Medicina da Universidade de Sao 
      Paulo, Sao Paulo, Brazil.
FAU - Murai, Igor H
AU  - Murai IH
AUID- ORCID: 0000-0002-0077-5004
AD  - Rheumatology Division, Hospital das Clinicas da Faculdade de Medicina da 
      Universidade de Sao Paulo (HCFMUSP), Faculdade de Medicina da Universidade de Sao 
      Paulo, Sao Paulo, Brazil.
FAU - Reis, Bruna Z
AU  - Reis BZ
AUID- ORCID: 0000-0001-8726-8699
AD  - Rheumatology Division, Hospital das Clinicas da Faculdade de Medicina da 
      Universidade de Sao Paulo (HCFMUSP), Faculdade de Medicina da Universidade de Sao 
      Paulo, Sao Paulo, Brazil.
FAU - Sales, Lucas P
AU  - Sales LP
AD  - Rheumatology Division, Hospital das Clinicas da Faculdade de Medicina da 
      Universidade de Sao Paulo (HCFMUSP), Faculdade de Medicina da Universidade de Sao 
      Paulo, Sao Paulo, Brazil.
FAU - Santos, Mayara D
AU  - Santos MD
AD  - Rheumatology Division, Hospital das Clinicas da Faculdade de Medicina da 
      Universidade de Sao Paulo (HCFMUSP), Faculdade de Medicina da Universidade de Sao 
      Paulo, Sao Paulo, Brazil.
FAU - Pinto, Ana J
AU  - Pinto AJ
AD  - Applied Physiology & Nutrition Research Group, Faculdade de Medicina da 
      Universidade de Sao Paulo, Sao Paulo, Brazil.
FAU - Goessler, Karla F
AU  - Goessler KF
AD  - Applied Physiology & Nutrition Research Group, Faculdade de Medicina da 
      Universidade de Sao Paulo, Sao Paulo, Brazil.
FAU - Duran, Camila S C
AU  - Duran CSC
AD  - Rheumatology Division, Hospital das Clinicas da Faculdade de Medicina da 
      Universidade de Sao Paulo (HCFMUSP), Faculdade de Medicina da Universidade de Sao 
      Paulo, Sao Paulo, Brazil.
FAU - Silva, Carla B R
AU  - Silva CBR
AD  - Rheumatology Division, Hospital das Clinicas da Faculdade de Medicina da 
      Universidade de Sao Paulo (HCFMUSP), Faculdade de Medicina da Universidade de Sao 
      Paulo, Sao Paulo, Brazil.
FAU - Franco, Andre S
AU  - Franco AS
AD  - Rheumatology Division, Hospital das Clinicas da Faculdade de Medicina da 
      Universidade de Sao Paulo (HCFMUSP), Faculdade de Medicina da Universidade de Sao 
      Paulo, Sao Paulo, Brazil.
FAU - Macedo, Marina B
AU  - Macedo MB
AD  - Rheumatology Division, Hospital das Clinicas da Faculdade de Medicina da 
      Universidade de Sao Paulo (HCFMUSP), Faculdade de Medicina da Universidade de Sao 
      Paulo, Sao Paulo, Brazil.
FAU - Dalmolin, Henrique H H
AU  - Dalmolin HHH
AD  - Rheumatology Division, Hospital das Clinicas da Faculdade de Medicina da 
      Universidade de Sao Paulo (HCFMUSP), Faculdade de Medicina da Universidade de Sao 
      Paulo, Sao Paulo, Brazil.
FAU - Baggio, Janaina
AU  - Baggio J
AD  - Rheumatology Division, Hospital das Clinicas da Faculdade de Medicina da 
      Universidade de Sao Paulo (HCFMUSP), Faculdade de Medicina da Universidade de Sao 
      Paulo, Sao Paulo, Brazil.
FAU - Balbi, Guilherme G M
AU  - Balbi GGM
AD  - Rheumatology Division, Hospital das Clinicas da Faculdade de Medicina da 
      Universidade de Sao Paulo (HCFMUSP), Faculdade de Medicina da Universidade de Sao 
      Paulo, Sao Paulo, Brazil.
FAU - Antonangelo, Leila
AU  - Antonangelo L
AD  - Clinical Pathology Division, Hospital das Clinicas HCFMUSP, Faculdade de Medicina 
      da Universidade de Sao Paulo,, Sao Paulo, Brazil.
FAU - Caparbo, Valeria F
AU  - Caparbo VF
AD  - Rheumatology Division, Hospital das Clinicas da Faculdade de Medicina da 
      Universidade de Sao Paulo (HCFMUSP), Faculdade de Medicina da Universidade de Sao 
      Paulo, Sao Paulo, Brazil.
FAU - Gualano, Bruno
AU  - Gualano B
AUID- ORCID: 0000-0001-7100-8681
AD  - Applied Physiology & Nutrition Research Group, Faculdade de Medicina da 
      Universidade de Sao Paulo, Sao Paulo, Brazil.
AD  - Food Research Center, Universidade de Sao Paulo, Sao Paulo, Brazil.
FAU - Pereira, Rosa M R
AU  - Pereira RMR
AUID- ORCID: 0000-0002-3723-5028
AD  - Rheumatology Division, Hospital das Clinicas da Faculdade de Medicina da 
      Universidade de Sao Paulo (HCFMUSP), Faculdade de Medicina da Universidade de Sao 
      Paulo, Sao Paulo, Brazil.
LA  - eng
SI  - ClinicalTrials.gov/NCT04449718
GR  - 2020/11102-2/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/
GR  - 305556/2017-7/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/
PT  - Journal Article
PT  - Multicenter Study
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Clin Nutr
JT  - The American journal of clinical nutrition
JID - 0376027
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vitamins)
RN  - 1C6V77QF41 (Cholecalciferol)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Brazil
MH  - COVID-19/immunology
MH  - Chemokines/*drug effects
MH  - Cholecalciferol/*administration & dosage
MH  - Cytokines/*drug effects
MH  - Double-Blind Method
MH  - Female
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*drug effects
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/blood
MH  - Male
MH  - Middle Aged
MH  - SARS-CoV-2/immunology
MH  - Vascular Endothelial Growth Factor A/*drug effects
MH  - Vitamins/*administration & dosage
MH  - *COVID-19 Drug Treatment
PMC - PMC8807215
OTO - NOTNLM
OT  - SARS-CoV-2
OT  - acute-phase reactants
OT  - immune response
OT  - inflammation
OT  - vitamin D
EDAT- 2022/01/13 06:00
MHDA- 2022/03/12 06:00
PMCR- 2023/01/21
CRDT- 2022/01/12 17:29
PHST- 2021/08/06 00:00 [received]
PHST- 2021/12/27 00:00 [accepted]
PHST- 2022/01/13 06:00 [pubmed]
PHST- 2022/03/12 06:00 [medline]
PHST- 2022/01/12 17:29 [entrez]
PHST- 2023/01/21 00:00 [pmc-release]
AID - S0002-9165(22)00196-4 [pii]
AID - 10.1093/ajcn/nqab426 [doi]
PST - ppublish
SO  - Am J Clin Nutr. 2022 Mar 4;115(3):790-798. doi: 10.1093/ajcn/nqab426.