PMID- 35021727
OWN - NLM
STAT- MEDLINE
DCOM- 20220128
LR  - 20220128
IS  - 2576-6422 (Electronic)
IS  - 2576-6422 (Linking)
VI  - 3
IP  - 8
DP  - 2020 Aug 17
TI  - GSH-ZnS Nanoparticles Exhibit High-Efficiency and Broad-Spectrum Antiviral 
      Activities via Multistep Inhibition Mechanisms.
PG  - 4809-4819
LID - 10.1021/acsabm.0c00332 [doi]
AB  - Despite the good biocompatibility and antibacterial activity of zinc sulfide 
      nanoparticles (ZnS NPs), whether they possess antiviral activity is still 
      unclear. Here, GSH-modified ZnS NPs (GSH-ZnS NPs) were synthesized and their 
      significant antiviral activity was demonstrated using the Arteriviridae family 
      RNA virus, porcine reproductive and respiratory syndrome virus (PRRSV), as a 
      model. Mechanistically, GSH-ZnS NPs were shown to reduce PRRSV-induced ROS 
      production to prevent PRRSV multiplication, with no activating effect on the 
      interferon (IFN) signal pathway, the first defense line against virus infection. 
      Furthermore, isobaric tags for relative and absolute quantification (iTRAQ)-based 
      quantitative proteomic analysis of GSH-ZnS NP-treated cells revealed the 
      involvement of numerous crucial proteins in virus proliferation, with vitronectin 
      (VTN) being confirmed as an efficient PRRSV antagonist here. Furthermore, GSH-ZnS 
      NPs were found to have potent antiviral effects on the Herpesviridae family DNA 
      virus, pseudorabies virus (PRV), the Coronaviridae family positive-sense RNA 
      virus, porcine epidemic diarrhea virus (PEDV), and the Rhabdoviridae family 
      negative-stranded RNA virus, vesicular stomatitis virus (VSV), indicating their 
      broad-spectrum antiviral activity against viruses from different families with 
      various genome types. Overall, GSH-ZnS NP is a prospective candidate for the 
      development of antiviral nanomaterials and may serve as a model for investigation 
      of potential host restriction factors in combination with proteomics.
FAU - Zhou, Yanrong
AU  - Zhou Y
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University, Wuhan 430070, P. R. China.
AD  - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, 
      P. R. China.
FAU - Tong, Ting
AU  - Tong T
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University, Wuhan 430070, P. R. China.
AD  - College of Science, Huazhong Agricultural University, Wuhan 430070, P. R. China.
AD  - College of Resources and Environment, Huazhong Agricultural University, Wuhan 
      430070, P. R. China.
FAU - Jiang, Xiaohan
AU  - Jiang X
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University, Wuhan 430070, P. R. China.
AD  - College of Science, Huazhong Agricultural University, Wuhan 430070, P. R. China.
FAU - Fang, Liurong
AU  - Fang L
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University, Wuhan 430070, P. R. China.
AD  - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, 
      P. R. China.
FAU - Wu, Yuan
AU  - Wu Y
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University, Wuhan 430070, P. R. China.
AD  - College of Science, Huazhong Agricultural University, Wuhan 430070, P. R. China.
FAU - Liang, Jiangong
AU  - Liang J
AUID- ORCID: 0000-0002-8210-0210
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University, Wuhan 430070, P. R. China.
AD  - College of Science, Huazhong Agricultural University, Wuhan 430070, P. R. China.
AD  - College of Resources and Environment, Huazhong Agricultural University, Wuhan 
      430070, P. R. China.
FAU - Xiao, Shaobo
AU  - Xiao S
AUID- ORCID: 0000-0003-0023-9188
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University, Wuhan 430070, P. R. China.
AD  - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, 
      P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200713
PL  - United States
TA  - ACS Appl Bio Mater
JT  - ACS applied bio materials
JID - 101729147
RN  - 0 (Antiviral Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Sulfides)
RN  - 0 (Zinc Compounds)
RN  - GAN16C9B8O (Glutathione)
RN  - KPS085631O (zinc sulfide)
SB  - IM
MH  - Animals
MH  - Antiviral Agents/*pharmacology
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Glutathione/*chemistry
MH  - Microbial Sensitivity Tests
MH  - Nanoparticles/*chemistry
MH  - Reactive Oxygen Species/metabolism
MH  - Sulfides/*chemistry
MH  - Viruses/classification/*drug effects
MH  - Zinc Compounds/*chemistry
OTO - NOTNLM
OT  - antiviral nanomaterial
OT  - coronavirus
OT  - isobaric tags for relative and absolute quantification
OT  - porcine reproductive and respiratory syndrome virus
OT  - reactive oxygen species
OT  - zinc sulfide
EDAT- 2020/08/17 00:00
MHDA- 2022/01/29 06:00
CRDT- 2022/01/13 05:32
PHST- 2022/01/13 05:32 [entrez]
PHST- 2020/08/17 00:00 [pubmed]
PHST- 2022/01/29 06:00 [medline]
AID - 10.1021/acsabm.0c00332 [doi]
PST - ppublish
SO  - ACS Appl Bio Mater. 2020 Aug 17;3(8):4809-4819. doi: 10.1021/acsabm.0c00332. Epub 
      2020 Jul 13.