PMID- 35021735
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220210
IS  - 2576-6422 (Electronic)
IS  - 2576-6422 (Linking)
VI  - 3
IP  - 8
DP  - 2020 Aug 17
TI  - Cytocompatibility of Mats Prepared from Different Electrospun Polymer Nanofibers.
PG  - 4912-4921
LID - 10.1021/acsabm.0c00426 [doi]
AB  - Mats of cytocompatible polymer fibers are needed as scaffolds in tissue 
      engineering or as wound healing supports. Most recently, they have emerged as 
      matrix-material to allow for in situ chemo- and biosensing inside intact tissue 
      fragments or surrogates. Electrospinning of such fibers from polymer solutions 
      provides extended options to control the structural and functional properties of 
      the resulting fiber mats. We have prepared electrospun polymeric fiber mats from 
      poly(lactic acid) (PLA), polystyrene (PS), and poly(vinyl pyrrolidone) (PVP) with 
      two different fiber densities. Mats and individual fibers were characterized with 
      respect to their dimensions, morphology, and their compatibility with human 
      keratinocytes (HaCaT) selected as a biological model. Microscopic inspection 
      revealed that HaCaT cells were viable on mats from all three polymers with only a 
      negligible fraction of dead cells, similar to planar control surfaces. Growth in 
      the presence of the fiber mats did not alter cellular metabolism (ATP, redox 
      state) and did not induce significant production of cytokines (interleukin-6 
      (IL-6); monocyte chemoattractant protein-1 (MCP-1)). However, we did observe that 
      fiber density changed the overall topography of the resulting mats and led to 
      differences in the establishment of continuous cell sheets. In conclusion, the 
      findings support the suitability of electrospun polymeric fiber mats made from 
      PLA, PS, or PVP as potential biocompatible matrices for future two-dimensional 
      (2D) or three-dimensional (3D) sensing of vital parameters from tissue in health 
      and disease.
FAU - Wongkaew, Nongnoot
AU  - Wongkaew N
AD  - Institute for Analytical Chemistry, Chemo- and Biosensors, Faculty of Chemistry 
      and Pharmacy, University of Regensburg, Universitatsstrasse 31, 93053 Regensburg, 
      Germany.
FAU - Simsek, Marcel
AU  - Simsek M
AUID- ORCID: 0000-0003-1288-8337
AD  - Institute for Analytical Chemistry, Chemo- and Biosensors, Faculty of Chemistry 
      and Pharmacy, University of Regensburg, Universitatsstrasse 31, 93053 Regensburg, 
      Germany.
FAU - Heider, Judith
AU  - Heider J
AD  - Department of Dermatology, University Medical Center Regensburg, 
      Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany.
FAU - Wegener, Joachim
AU  - Wegener J
AD  - Institute for Analytical Chemistry, Chemo- and Biosensors, Faculty of Chemistry 
      and Pharmacy, University of Regensburg, Universitatsstrasse 31, 93053 Regensburg, 
      Germany.
AD  - Fraunhofer Research Institution for Microsystems and Solid State Technologies, 
      Universitatsstrasse 31, 93053 Regensburg, Germany.
FAU - Baeumner, Antje J
AU  - Baeumner AJ
AUID- ORCID: 0000-0001-7148-3423
AD  - Institute for Analytical Chemistry, Chemo- and Biosensors, Faculty of Chemistry 
      and Pharmacy, University of Regensburg, Universitatsstrasse 31, 93053 Regensburg, 
      Germany.
FAU - Schreml, Stephan
AU  - Schreml S
AD  - Department of Dermatology, University Medical Center Regensburg, 
      Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany.
FAU - Stolwijk, Judith A
AU  - Stolwijk JA
AUID- ORCID: 0000-0003-0474-9417
AD  - Institute for Analytical Chemistry, Chemo- and Biosensors, Faculty of Chemistry 
      and Pharmacy, University of Regensburg, Universitatsstrasse 31, 93053 Regensburg, 
      Germany.
AD  - Department of Dermatology, University Medical Center Regensburg, 
      Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany.
LA  - eng
PT  - Journal Article
DEP - 20200723
PL  - United States
TA  - ACS Appl Bio Mater
JT  - ACS applied bio materials
JID - 101729147
SB  - IM
OTO - NOTNLM
OT  - cytocompatibility
OT  - cytokine production
OT  - electrospun fibers
OT  - human keratinocytes
OT  - polymer nanofibers
EDAT- 2020/08/17 00:00
MHDA- 2020/08/17 00:01
CRDT- 2022/01/13 05:32
PHST- 2022/01/13 05:32 [entrez]
PHST- 2020/08/17 00:00 [pubmed]
PHST- 2020/08/17 00:01 [medline]
AID - 10.1021/acsabm.0c00426 [doi]
PST - ppublish
SO  - ACS Appl Bio Mater. 2020 Aug 17;3(8):4912-4921. doi: 10.1021/acsabm.0c00426. Epub 
      2020 Jul 23.