PMID- 35022735
OWN - NLM
STAT- MEDLINE
DCOM- 20230125
LR  - 20230201
IS  - 1945-2403 (Electronic)
IS  - 0146-4760 (Linking)
VI  - 46
IP  - 9
DP  - 2023 Jan 24
TI  - Qualitative Screening of Amphetamine- and Ketamine-Type Abuse Drugs in Urine 
      Employing Dual Mode Extraction Column by Liquid Chromatography-Tandem Mass 
      Spectrometry (LC-MS-MS).
PG  - 1045-1052
LID - 10.1093/jat/bkac004 [doi]
AB  - This manuscript reported a fast and rapid qualitative screening method for abuse 
      drugs in urine by liquid chromatography-tandem mass spectrometry (LC-MS-MS). The 
      scope of the abuse drugs under investigation included methamphetamine (MA), 
      amphetamine (AMP), methylenedioxymethamphetamine (MDMA), 
      methylenedioxyamphetamine (MDA), paramethoxymethamphetamine (PMMA), ephedrine, 
      pseudoephedrine, ketamine (KET), deschloroketamine (DCK), 
      2-fluorodeschloroketamine (2 F-DCK) and deschloro-N-ethylketamine (2-oxo-PCE). 
      The method employed a dual mode extraction (DME) column as a novel clean-up 
      method for the urine matrix. To an aliquot of 0.2 mL urine, internal standards 
      (ISTDs) and 0.4 mL of acidified methanol were added. After vortex and 
      centrifugation, the supernatant was passed through a DME column before LC-MS-MS 
      analysis. Chromatographic separation was achieved with a C18 column by gradient 
      elution. The limits of detection (LODs) for MA, AMP, MDMA, MDA and PMMA were 
      3 ng/mL, whereas those for ephedrine and pseudoephedrine were 10 ng/mL and those 
      for KET, DCK, 2 F-DCK and 2-oxo-PCE were 1 ng/mL. The matrix effects ranged from 
      -12% to 7% (%CV from 4% to 19%). This method is fit for the intended purpose for 
      forensic toxicology, as well as for forensic analysis of drugs facilitating 
      sexual assault and other criminal acts.
CI  - (c) The Author(s) 2022. Published by Oxford University Press. All rights reserved. 
      For permissions, please e-mail: journals.permissions@oup.com.
FAU - Wong, George Fai
AU  - Wong GF
AUID- ORCID: 0000-0002-3643-827X
AD  - Forensic Science Division, Government Laboratory of Hong Kong, 7/F, Ho Man Tin 
      Government offices, 88 Chung Hau Street, Homantin, Hong Kong.
FAU - Lee, Wing-Man
AU  - Lee WM
AD  - Forensic Science Division, Government Laboratory of Hong Kong, 7/F, Ho Man Tin 
      Government offices, 88 Chung Hau Street, Homantin, Hong Kong.
FAU - Li, Chi-Keung
AU  - Li CK
AD  - Forensic Science Division, Government Laboratory of Hong Kong, 7/F, Ho Man Tin 
      Government offices, 88 Chung Hau Street, Homantin, Hong Kong.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - 0 (2-fluorodeschloroketamine)
RN  - 0 (2-Oxo-PCE)
RN  - 690G0D6V8H (Ketamine)
RN  - GN83C131XS (Ephedrine)
RN  - 9011-14-7 (Polymethyl Methacrylate)
RN  - 7CUC9DDI9F (Pseudoephedrine)
RN  - 0 (paramethoxymethamphetamine)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - 0 (Illicit Drugs)
RN  - CK833KGX7E (Amphetamine)
SB  - IM
MH  - Humans
MH  - *N-Methyl-3,4-methylenedioxyamphetamine
MH  - *Ketamine
MH  - Chromatography, Liquid
MH  - Tandem Mass Spectrometry/methods
MH  - Ephedrine
MH  - Polymethyl Methacrylate/analysis
MH  - Pseudoephedrine
MH  - Substance Abuse Detection/methods
MH  - *3,4-Methylenedioxyamphetamine/urine
MH  - *Substance-Related Disorders
MH  - *Illicit Drugs
MH  - Amphetamine/urine
EDAT- 2022/01/14 06:00
MHDA- 2023/01/26 06:00
CRDT- 2022/01/13 06:14
PHST- 2021/08/06 00:00 [received]
PHST- 2021/12/05 00:00 [revised]
PHST- 2022/01/12 00:00 [accepted]
PHST- 2022/01/14 06:00 [pubmed]
PHST- 2023/01/26 06:00 [medline]
PHST- 2022/01/13 06:14 [entrez]
AID - 6504780 [pii]
AID - 10.1093/jat/bkac004 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2023 Jan 24;46(9):1045-1052. doi: 10.1093/jat/bkac004.