PMID- 35023060
OWN - NLM
STAT- MEDLINE
DCOM- 20220826
LR  - 20220826
IS  - 1993-0402 (Electronic)
IS  - 1672-0415 (Linking)
VI  - 28
IP  - 9
DP  - 2022 Sep
TI  - Protective Effect of Shenfu Injection on Vascular Endothelial Damage in a Porcine 
      Model of Hemorrhagic Shock.
PG  - 794-801
LID - 10.1007/s11655-021-2876-x [doi]
AB  - OBJECTIVE: To investigate the effects of Shenfu Injection (, SFI) on endothelial 
      damage in a porcine model of hemorrhagic shock (HS). METHODS: After being bled to 
      a mean arterial pressure of 40+/-3 mm Hg and held for 60 min, 32 pigs were treated 
      with a venous injection of either shed blood (transfusion group), shed blood and 
      saline (saline group), shed blood and SFI (SFI group) or without resuscitation 
      (sham group). Venous blood samples were collected and analyzed at baseline and 0, 
      1, 2, 4, and 6 h after HS. Tumor necrosis factor-alpha (TNF-alpha), serum interleuking 
      (IL)-6, and IL-10 levels were measured by enzyme-linked immunosorbent assay 
      (ELISA); expressions of vascular cell adhesion molecule-1 (VCAM-1), intercellular 
      adhesion molecule 1 (ICAM -1), von Willebrand factor (vWF), plasminogen activator 
      inhibitor-1 (PAI-1) and Bcl-2, Bax, and caspase-3 proteins were determined by 
      Western blot. RESULTS: The serum level of TNF-alpha in the SFI group was 
      significantly lower than in the other groups at 0, 1, and 2 h after HS, while the 
      level of IL-6 was lower at 4 and 6 h compared with the saline group (P<0.01 or 
      P<0.05). The concentration of serum IL-10 was significantly higher in the SFI 
      group than in the other groups at 0, 1, 4, and 6 h after HS (P<0.01). Western 
      blot and immunohistochemistry of vascular tissue showed that the expression of 
      caspase-3 was downregulated, and that of Bcl-2 and Bax was upregulated in the SFI 
      group compared to other groups (P<0.05). CONCLUSION: SFI attenuated endothelial 
      injury in the porcine model of HS by inhibiting cell apoptosis, suppressing the 
      formation of proinflammatory cytokines, and reducing endothelial activation.
CI  - (c) 2021. The Chinese Journal of Integrated Traditional and Western Medicine Press 
      and Springer-Verlag GmbH Germany, part of Springer Nature.
FAU - Zhang, Ming-Qing
AU  - Zhang MQ
AD  - Department of Emergency Medicine, Beijing Friendship Hospital, Capital Medical 
      University, Beijing, 100050, China.
FAU - Zhang, Qiang
AU  - Zhang Q
AD  - Department of Critical Care Medicine, Peking University Third Hospital, Beijing, 
      100191, China.
FAU - Yuan, Wei
AU  - Yuan W
AD  - Department of Emergency Medicine, Beijing Chaoyang Hospital, Capital Medical 
      University, Beijing, 100020, China.
FAU - Wu, Jun-Yuan
AU  - Wu JY
AD  - Department of Emergency Medicine, Beijing Chaoyang Hospital, Capital Medical 
      University, Beijing, 100020, China.
FAU - Liang, Yong
AU  - Liang Y
AD  - Department of Emergency Medicine, Beijing Chaoyang Hospital, Capital Medical 
      University, Beijing, 100020, China.
FAU - Qin, Hong-Jie
AU  - Qin HJ
AD  - Department of Emergency Medicine, Beijing Luhe Hospital, Capital Medical 
      University, Beijing, 101199, China.
FAU - Li, Chun-Sheng
AU  - Li CS
AD  - Department of Emergency Medicine, Beijing Friendship Hospital, Capital Medical 
      University, Beijing, 100050, China. lcscyyy@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220113
PL  - China
TA  - Chin J Integr Med
JT  - Chinese journal of integrative medicine
JID - 101181180
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Shen-Fu)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 130068-27-8 (Interleukin-10)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Caspase 3/metabolism
MH  - Drugs, Chinese Herbal
MH  - Interleukin-10
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - *Shock, Hemorrhagic/drug therapy
MH  - Swine
MH  - *Tumor Necrosis Factor-alpha/metabolism
MH  - bcl-2-Associated X Protein/metabolism
OTO - NOTNLM
OT  - Chinese medicine
OT  - Shenfu Injection
OT  - hemorrhagic shock
OT  - vascular endothelial damage
EDAT- 2022/01/14 06:00
MHDA- 2022/08/27 06:00
CRDT- 2022/01/13 06:23
PHST- 2021/03/30 00:00 [accepted]
PHST- 2022/01/14 06:00 [pubmed]
PHST- 2022/08/27 06:00 [medline]
PHST- 2022/01/13 06:23 [entrez]
AID - 10.1007/s11655-021-2876-x [pii]
AID - 10.1007/s11655-021-2876-x [doi]
PST - ppublish
SO  - Chin J Integr Med. 2022 Sep;28(9):794-801. doi: 10.1007/s11655-021-2876-x. Epub 
      2022 Jan 13.