PMID- 3502389
OWN - NLM
STAT- MEDLINE
DCOM- 19880610
LR  - 20161021
IS  - 0125-877X (Print)
IS  - 0125-877X (Linking)
VI  - 5
IP  - 2
DP  - 1987 Dec
TI  - Measurement of immune complexes with the liquid phase C1q binding assay: ten 
      years experience in a routine diagnostic laboratory.
PG  - 149-54
AB  - We describe our 10 years experience in assaying over 15,000 clinical specimens 
      for immune complexes (IC) using the C1q binding assay. Normal ranges were 
      initially established using a large panel of blood donor sera and precision of 
      the assay was optimized by inclusion of heat aggregated IgG (HAGG) as standards. 
      Nevertheless some variability was observed due to variation in C1q binding from 
      batch to batch and with aging of this reagent. In an empirically selected 2 year 
      period involving over 3,000 clinical specimens, 25% had elevated concentrations 
      of IC. Of these the majority were from patients with rheumatoid arthritis (RA), 
      systemic lupus erythematosus (SLE), other connective tissue disorders, infective 
      endocarditis (IE), diffuse interstitial lung disease (DILD) and vasculitis 
      (VASC). In RA, IE and VASC, significant correlations were observed between 
      concentrations of IC and rheumatoid factor (RF) and the addition of a purified 
      monoclonal RF to normal serum caused increased C1q binding. Longitudinal studies 
      in RA and IE demonstrated a striking decline in IC in response to effective 
      treatment. We conclude that the measurement of IC provides little additional 
      useful diagnostic information in those diseases associated with high levels of RF 
      but appears more useful in disorders such as SLE, IE and DILD in which RF is 
      absent or present in low concentration. Sequential monitoring of IC in RA and IE 
      reflects response to treatment.
FAU - Roberts-Thomson, P J
AU  - Roberts-Thomson PJ
AD  - Department of Clinical Immunology, Flinders Medical Centre, Adelaide, South 
      Australia.
FAU - Neoh, S H
AU  - Neoh SH
FAU - Kennedy, A
AU  - Kennedy A
FAU - Smith, M D
AU  - Smith MD
FAU - O'Donnell, J
AU  - O'Donnell J
FAU - Bradley, J
AU  - Bradley J
LA  - eng
PT  - Journal Article
PL  - Thailand
TA  - Asian Pac J Allergy Immunol
JT  - Asian Pacific journal of allergy and immunology
JID - 8402034
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Complement C1)
RN  - 80295-33-6 (Complement C1q)
RN  - EC 3.- (Complement Activating Enzymes)
SB  - IM
MH  - Antigen-Antibody Complex/*analysis
MH  - Autoimmune Diseases/immunology
MH  - *Complement Activating Enzymes/metabolism
MH  - *Complement C1/metabolism
MH  - Complement C1q
MH  - Cross-Sectional Studies
MH  - Evaluation Studies as Topic
MH  - Humans
MH  - Retrospective Studies
MH  - Rheumatic Diseases/immunology
EDAT- 1987/12/01 00:00
MHDA- 1987/12/01 00:01
CRDT- 1987/12/01 00:00
PHST- 1987/12/01 00:00 [pubmed]
PHST- 1987/12/01 00:01 [medline]
PHST- 1987/12/01 00:00 [entrez]
PST - ppublish
SO  - Asian Pac J Allergy Immunol. 1987 Dec;5(2):149-54.