PMID- 35025461
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220113
IS  - 2576-6422 (Electronic)
IS  - 2576-6422 (Linking)
VI  - 3
IP  - 7
DP  - 2020 Jul 20
TI  - Elucidating the Molecular Interactions of Encapsulated Doxorubicin within a 
      Nonionic, Thermoresponsive Polyester Coacervate.
PG  - 4626-4634
LID - 10.1021/acsabm.0c00507 [doi]
AB  - Thermoresponsive polymers that display a lower critical solution temperature 
      (LCST) are attractive drug delivery systems (DDSs) due to their potential to 
      encapsulate and release therapeutics in a sustained manner as a function of 
      temperature input. To attain the full potential of such DDSs, methods that 
      illustrate the details of drug-polymer interactions are necessary. Here, we 
      synthesized a nonionic, coacervate-forming, thermoresponsive polyester to 
      encapsulate doxorubicin (Dox) and used solution state NMR spectroscopy and 
      fluorescence microscopy techniques to probe the interactions between the polymer 
      and Dox at the molecular level. The incomplete dehydration provides a matrix for 
      encapsulation of sensitive therapeutics and preserving their activity, while the 
      low hysteresis property of the polyester provides rapid transition from soluble 
      to coacervate phase. Saturation transfer difference (STD) NMR revealed the 
      Dox-polymer interactions within the coacervates. (1)H-(1)H nuclear Overhauser 
      effect spectroscopy (NOESY) cross-peak differences of Dox confirmed the 
      Dox-polymer interactions. Diffusion-ordered spectroscopy (DOSY) revealed the 
      slower diffusion rate of Dox in the presence of polyester coacervates. These 
      studies illustrate how the state of the polyester (below and above LCST) affects 
      the polyester-Dox interactions and offers details of the specific functional 
      groups involved in these interactions. Our results provide a framework for future 
      investigations aimed at characterizing fundamental interactions in polymer-based 
      DDSs.
FAU - Kundu, Mangaldeep
AU  - Kundu M
AUID- ORCID: 0000-0003-2555-0474
AD  - Department of Polymer Science, The University of Akron, Akron, Ohio 44325, United 
      States.
FAU - Morris, Daniel L
AU  - Morris DL
AD  - Department of Chemistry and Biochemistry, The University of Akron, Akron, Ohio 
      44325, United States.
FAU - Cruz, Megan A
AU  - Cruz MA
AD  - Department of Polymer Science, The University of Akron, Akron, Ohio 44325, United 
      States.
FAU - Miyoshi, Toshikazu
AU  - Miyoshi T
AUID- ORCID: 0000-0001-8344-9687
AD  - Department of Polymer Science, The University of Akron, Akron, Ohio 44325, United 
      States.
FAU - Leeper, Thomas C
AU  - Leeper TC
AD  - College of Science and Mathematics, Kennesaw State University, Kennesaw, Georgia 
      30144, United States.
FAU - Joy, Abraham
AU  - Joy A
AUID- ORCID: 0000-0001-7781-3817
AD  - Department of Polymer Science, The University of Akron, Akron, Ohio 44325, United 
      States.
LA  - eng
PT  - Journal Article
DEP - 20200708
PL  - United States
TA  - ACS Appl Bio Mater
JT  - ACS applied bio materials
JID - 101729147
SB  - IM
OTO - NOTNLM
OT  - controlled release polyester
OT  - drug delivery system
OT  - nonionic coacervate
OT  - polyester coacervate
OT  - smart polymers
OT  - stimuli responsive materials
OT  - thermoresponsive polymers
EDAT- 2020/07/20 00:00
MHDA- 2020/07/20 00:01
CRDT- 2022/01/13 17:14
PHST- 2022/01/13 17:14 [entrez]
PHST- 2020/07/20 00:00 [pubmed]
PHST- 2020/07/20 00:01 [medline]
AID - 10.1021/acsabm.0c00507 [doi]
PST - ppublish
SO  - ACS Appl Bio Mater. 2020 Jul 20;3(7):4626-4634. doi: 10.1021/acsabm.0c00507. Epub 
      2020 Jul 8.