PMID- 35025965
OWN - NLM
STAT- MEDLINE
DCOM- 20220218
LR  - 20220218
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 17
IP  - 1
DP  - 2022
TI  - Thermal stress affects proliferation and differentiation of turkey satellite 
      cells through the mTOR/S6K pathway in a growth-dependent manner.
PG  - e0262576
LID - 10.1371/journal.pone.0262576 [doi]
LID - e0262576
AB  - Satellite cells (SCs) are stem cells responsible for post-hatch muscle growth 
      through hypertrophy and in birds are sensitive to thermal stress during the first 
      week after hatch. The mechanistic target of rapamycin (mTOR) signaling pathway, 
      which is highly responsive to thermal stress in differentiating turkey pectoralis 
      major (p. major) muscle SCs, regulates protein synthesis and the activities of 
      SCs through a downstream effector, S6 kinase (S6K). The objectives of this study 
      were: 1) to determine the effect of heat (43 degrees C) and cold (33 degrees C) stress on 
      activity of the mTOR/S6K pathway in SCs isolated from the p. major muscle of 
      one-week-old faster-growing modern commercial (NC) turkeys compared to those from 
      slower-growing Randombred Control Line 2 (RBC2) turkeys, and 2) to assess the 
      effect of mTOR knockdown on the proliferation, differentiation, and expression of 
      myogenic regulatory factors of the SCs. Heat stress increased phosphorylation of 
      both mTOR and S6K in both turkey lines, with greater increases observed in the 
      RBC2 line. With cold stress, greater reductions in mTOR and S6K phosphorylation 
      were observed in the NC line. Early knockdown of mTOR decreased proliferation, 
      differentiation, and expression of myoblast determination protein 1 and myogenin 
      in both lines independent of temperature, with the RBC2 line showing greater 
      reductions in proliferation and differentiation than the NC line at 38 degrees  and 43 degrees C. 
      Proliferating SCs are more dependent on mTOR/S6K-mediated regulation than 
      differentiating SCs. Thus, thermal stress can affect breast muscle hypertrophic 
      potential by changing satellite cell proliferation and differentiation, in part, 
      through the mTOR/S6K pathway in a growth-dependent manner. These changes may 
      result in irreversible effects on the development and growth of the turkey p. 
      major muscle.
FAU - Xu, Jiahui
AU  - Xu J
AUID- ORCID: 0000-0003-2780-9267
AD  - Department of Animal Sciences, The Ohio State University, Wooster, OH, United 
      States of America.
FAU - Strasburg, Gale M
AU  - Strasburg GM
AUID- ORCID: 0000-0002-3897-4340
AD  - Department of Food Science and Human Nutrition, Michigan State University, East 
      Lansing, MI, United States of America.
FAU - Reed, Kent M
AU  - Reed KM
AD  - Department of Veterinary and Biomedical Sciences, University of Minnesota, St. 
      Paul, MN, United States of America.
FAU - Velleman, Sandra G
AU  - Velleman SG
AUID- ORCID: 0000-0001-8291-847X
AD  - Department of Animal Sciences, The Ohio State University, Wooster, OH, United 
      States of America.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220113
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (MyoD Protein)
RN  - 0 (MyoD1 myogenic differentiation protein)
RN  - 0 (Myogenin)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/physiology
MH  - Cell Proliferation/physiology
MH  - Cold-Shock Response/physiology
MH  - Heat-Shock Response/*physiology
MH  - Intracellular Signaling Peptides and Proteins/metabolism
MH  - MyoD Protein/metabolism
MH  - Myogenin/metabolism
MH  - Protein Serine-Threonine Kinases/metabolism
MH  - Ribosomal Protein S6 Kinases/metabolism
MH  - Satellite Cells, Skeletal Muscle/*metabolism
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Temperature
MH  - Turkeys/*growth & development/metabolism
PMC - PMC8758067
COIS- The authors have declared that no competing interests exist.
EDAT- 2022/01/14 06:00
MHDA- 2022/02/19 06:00
PMCR- 2022/01/13
CRDT- 2022/01/13 17:30
PHST- 2021/08/10 00:00 [received]
PHST- 2021/12/29 00:00 [accepted]
PHST- 2022/01/13 17:30 [entrez]
PHST- 2022/01/14 06:00 [pubmed]
PHST- 2022/02/19 06:00 [medline]
PHST- 2022/01/13 00:00 [pmc-release]
AID - PONE-D-21-25935 [pii]
AID - 10.1371/journal.pone.0262576 [doi]
PST - epublish
SO  - PLoS One. 2022 Jan 13;17(1):e0262576. doi: 10.1371/journal.pone.0262576. 
      eCollection 2022.