PMID- 35028055
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220430
IS  - 2213-8862 (Electronic)
IS  - 1991-7902 (Print)
IS  - 1991-7902 (Linking)
VI  - 17
IP  - 1
DP  - 2022 Jan
TI  - Orthodontic force regulates metalloproteinase-3 promoter in osteoblasts and 
      transgenic mouse models.
PG  - 331-337
LID - 10.1016/j.jds.2021.11.015 [doi]
AB  - BACKGROUND/PURPOSE: Previously we demonstrated up-regulation of matrix 
      metalloproteinase-3 (MMP-3) in human osteoblasts under compression and in bony 
      specimens of experimental orthodontic tooth movement (OTM). Here, we studied the 
      temporal characteristics of compression stimulation in human and mouse osteoblast 
      cell lines, and generated a transgenic mouse model for assessing the MMP-3 
      expression during OTM. MATERIALS AND METHODS: We investigated MMP-3 expressions 
      in human and murine osteoblasts through RT-PCR and luciferase assay, after 
      compressive force loading. Inhibitors were added to identify the possible 
      mechanisms for signal transduction. A human MMP-3 promoter was isolated, cloned 
      and transfected to generate a transgenic mouse with a green fluorescent protein 
      reporter. OTM was then initiated to observe the location and time course of 
      transcriptional regulation of MMP-3 signals. RESULTS: We found changes in the 
      transcription of MMP-3 in response to mechanical force applied to both human and 
      mouse osteoblast cell lines, suggesting that the response is positive across 
      species. Cloned human MMP-3 promoter may cause the response of luciferase to 1% 
      compression. Moreover, p38 inhibitor exerted a down-regulatory effect on MMP-3 
      promoter expression, although the inhibitory effect didn't reach a significant 
      level. In the transgenic mouse OTM model, we again found increased expression of 
      MMP-3 in response to mechanical force loading around the periodontal ligament. 
      CONCLUSION: Mechanical force can stimulate MMP-3 expression, possibly through the 
      p38 MAPK pathway, with its strongest signal occurring at 24 h. The mechanical 
      responsiveness in MMP-3 promoter regions can be observed in both humans and 
      rodents in vitro and in vivo.
CI  - (c) 2021 Association for Dental Sciences of the Republic of China. Publishing 
      services by Elsevier B.V.
FAU - Hsu, Li-Fang
AU  - Hsu LF
AD  - Department of Dentistry, National Taiwan University Hospital, Hsin-Chu Branch, 
      Hsin-Chu, Taiwan.
AD  - Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan 
      University, Taipei, Taiwan.
FAU - Chang, Bei-En
AU  - Chang BE
AD  - Graduate Institute of Oral Biology, School of Dentistry, College of Medicine, 
      National Taiwan University, Taipei, Taiwan.
FAU - Tseng, Kuo-Jung
AU  - Tseng KJ
AD  - Graduate Institute of Oral Biology, School of Dentistry, College of Medicine, 
      National Taiwan University, Taipei, Taiwan.
FAU - Liao, Chih-Ching
AU  - Liao CC
AD  - Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan 
      University, Taipei, Taiwan.
FAU - Tsai, Shu-Chun
AU  - Tsai SC
AD  - Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan 
      University, Taipei, Taiwan.
FAU - Hsiao, Hsuan-Yi
AU  - Hsiao HY
AD  - Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan 
      University, Taipei, Taiwan.
FAU - Lin, Sheng-Chin
AU  - Lin SC
AD  - Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan 
      University, Taipei, Taiwan.
FAU - Liao, Pei-Wen
AU  - Liao PW
AD  - Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan 
      University, Taipei, Taiwan.
FAU - Chen, Yi-Jane
AU  - Chen YJ
AD  - School of Dentistry, National Taiwan University, Taipei, Taiwan.
AD  - Division of Orthodontics and Dentofacial Orthopedics, Dental Department, National 
      Taiwan University Hospital, Taipei, Taiwan.
FAU - Jane Yao, Chung-Chen
AU  - Jane Yao CC
AD  - Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan 
      University, Taipei, Taiwan.
AD  - Division of Orthodontics and Dentofacial Orthopedics, Dental Department, National 
      Taiwan University Hospital, Taipei, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20211204
PL  - Netherlands
TA  - J Dent Sci
JT  - Journal of dental sciences
JID - 101293181
PMC - PMC8740386
OTO - NOTNLM
OT  - Matrix metalloproteinase-3
OT  - Mechanical force
OT  - Orthodontic tooth movement
OT  - Transgenic mouse
EDAT- 2022/01/15 06:00
MHDA- 2022/01/15 06:01
PMCR- 2021/12/04
CRDT- 2022/01/14 06:02
PHST- 2021/11/16 00:00 [received]
PHST- 2021/11/20 00:00 [revised]
PHST- 2022/01/14 06:02 [entrez]
PHST- 2022/01/15 06:00 [pubmed]
PHST- 2022/01/15 06:01 [medline]
PHST- 2021/12/04 00:00 [pmc-release]
AID - S1991-7902(21)00281-6 [pii]
AID - 10.1016/j.jds.2021.11.015 [doi]
PST - ppublish
SO  - J Dent Sci. 2022 Jan;17(1):331-337. doi: 10.1016/j.jds.2021.11.015. Epub 2021 Dec 
      4.