PMID- 35029031
OWN - NLM
STAT- MEDLINE
DCOM- 20220526
LR  - 20220526
IS  - 1751-553X (Electronic)
IS  - 1751-5521 (Linking)
VI  - 44
IP  - 3
DP  - 2022 Jun
TI  - Stability of coagulation parameters in plasma samples at room temperature after 
      one freeze/thaw cycle.
PG  - 610-618
LID - 10.1111/ijlh.13794 [doi]
AB  - INTRODUCTION: Sample freezing is a part of routine laboratory tasks because some 
      coagulation parameters are analysed in batches to optimize reagent consumption. 
      The coagulation parameter stability in fresh and frozen samples has been 
      described, but data are scarcer after thawing. This study objective was to 
      determine the stability of the main coagulation parameters (from blood withdrawn 
      on siliconized CTAD tubes and double-centrifuged) after one freeze/thaw cycle to 
      generate procedures for appropriate handling, storage and testing. METHODS: 
      Prothrombin time (PT), activated partial thromboplastin time (aPTT), fibrinogen, 
      D-dimers, clotting factors (F), protein C, protein S, antithrombin, lupus 
      anticoagulant (LA)-sensitive aPTT and diluted-Russel's viper venom time (dRVVT) 
      were assessed in 60 plasma samples (n=30, normal range and n=30, outside the 
      normal range). Thirty samples from anticoagulated patients [unfractionated 
      heparin (UFH), low-molecular weight heparin (LMWH), apixaban or rivaroxaban] were 
      assessed using specific anticoagulant assays. Frozen samples were thawed, and 
      assays were performed at 15 min, 2, 4 and 6 h after thawing. The coagulation 
      parameter stability was assessed with the method of rejection limit. RESULTS: 
      After thawing, aPTT, PT, fibrinogen, D-dimers, FII, FV, FX, FIX, FXI, FXII, PC 
      and UFH anti-Xa activity remained stable for at least 6 h, FVII for 5 h, PS, AT, 
      dRVVT screen assay and LMWH anti-Xa activity for 4 h, and LA-sensitive aPTT and 
      apixaban-specific anti-Xa activity for 3 h. FVIII, dRVVT confirm assay and 
      rivaroxaban specific anti-Xa activity were stable for 2 h. CONCLUSION: These 
      results suggest that sample stability for some haemostasis assays is limited 
      after thawing.
CI  - (c) 2022 John Wiley & Sons Ltd.
FAU - Gaudard, Marion
AU  - Gaudard M
AD  - Service d'hematologie biologique, Centre hospitalier universitaire de 
      Clermont-Ferrand, Clermont-Ferrand, France.
FAU - Boissier, Elodie
AU  - Boissier E
AD  - Service d'hematologie biologique, Centre hospitalier universitaire de Nantes, 
      Nantes, France.
FAU - Talon, Laurie
AU  - Talon L
AD  - Service d'hematologie biologique, Centre hospitalier universitaire de 
      Clermont-Ferrand, Clermont-Ferrand, France.
FAU - Douxfils, Jonathan
AU  - Douxfils J
AD  - Department of Pharmacy, Namur Research Institute for Life Sciences, Namur 
      Thrombosis and Haemostasis Center, University of Namur, Namur, Belgium.
AD  - Qualiblood sa, Namur, Belgium.
FAU - Sapin, Anne-Francoise
AU  - Sapin AF
AD  - Service d'hematologie biologique, Centre hospitalier universitaire de 
      Clermont-Ferrand, Clermont-Ferrand, France.
FAU - Sinegre, Thomas
AU  - Sinegre T
AD  - Service d'hematologie biologique, Centre hospitalier universitaire de 
      Clermont-Ferrand, Clermont-Ferrand, France.
AD  - INRA UMR 1019, Universite Clermont Auvergne, Clermont-Ferrand, France.
FAU - Lebreton, Aurelien
AU  - Lebreton A
AUID- ORCID: 0000-0002-1650-0427
AD  - Service d'hematologie biologique, Centre hospitalier universitaire de 
      Clermont-Ferrand, Clermont-Ferrand, France.
AD  - INRA UMR 1019, Universite Clermont Auvergne, Clermont-Ferrand, France.
LA  - eng
PT  - Journal Article
DEP - 20220113
PL  - England
TA  - Int J Lab Hematol
JT  - International journal of laboratory hematology
JID - 101300213
RN  - 0 (Anticoagulants)
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 0 (Lupus Coagulation Inhibitor)
RN  - 9001-32-5 (Fibrinogen)
RN  - 9005-49-6 (Heparin)
RN  - 9NDF7JZ4M3 (Rivaroxaban)
SB  - IM
MH  - Anticoagulants/pharmacology/therapeutic use
MH  - *Antiphospholipid Syndrome
MH  - Blood Coagulation Tests/methods
MH  - Fibrinogen
MH  - Freezing
MH  - Heparin
MH  - Heparin, Low-Molecular-Weight
MH  - Humans
MH  - Lupus Coagulation Inhibitor
MH  - Partial Thromboplastin Time
MH  - *Rivaroxaban
MH  - Temperature
OTO - NOTNLM
OT  - anticoagulants
OT  - blood coagulation factors
OT  - freezing
OT  - laboratories
OT  - plasma
EDAT- 2022/01/15 06:00
MHDA- 2022/05/27 06:00
CRDT- 2022/01/14 06:24
PHST- 2021/12/16 00:00 [revised]
PHST- 2021/07/20 00:00 [received]
PHST- 2021/12/23 00:00 [accepted]
PHST- 2022/01/15 06:00 [pubmed]
PHST- 2022/05/27 06:00 [medline]
PHST- 2022/01/14 06:24 [entrez]
AID - 10.1111/ijlh.13794 [doi]
PST - ppublish
SO  - Int J Lab Hematol. 2022 Jun;44(3):610-618. doi: 10.1111/ijlh.13794. Epub 2022 Jan 
      13.