PMID- 35029100
OWN - NLM
STAT- MEDLINE
DCOM- 20230310
LR  - 20230414
IS  - 1764-1489 (Print)
IS  - 1764-1489 (Linking)
VI  - 55
IP  - 2
DP  - 2023 Mar
TI  - Qualitative and quantitative comparison of allergen component-specific to birch 
      and grass analyzed by ImmunoCAP assay and Euroline immunoblot test.
PG  - 68-77
LID - 10.23822/EurAnnACI.1764-1489.241 [doi]
AB  - Background.In the diagnostic work up of allergy, determining allergen 
      component-specific immunoglobulin E (IgE) is important for diagnosis, prognosis 
      and choice of treatment. The purpose of this study was to evaluate the 
      performance of the immunoblotting assay (Euroline) in detection of IgE antibodies 
      against timothy grass and birch pollen allergen components compared to 
      fluorescent enzyme assay (ImmunoCAP, Phadia 250). Methods. A total of 128 serum 
      samples from patients allergic to timothy grass and birch pollen were analysed. 
      The levels of IgE antibodies to timothy grass and birch pollen were measured 
      using Euroline DPA-Dx pollen 1 and ImmunoCAP assay. The two methods were then 
      compared on binary (positive vs negative), semi-quantitative (IgE classes) and 
      quantitative (concentration) levels. The two methods were also compared to 
      results from skin prick testing. Results. The Euroline method showed a positive 
      percentage agreement of 93% and negative percentage agreement of 94% with an 
      overall accuracy of 94% when compared to ImmunoCAP. Kappa analysis showed 
      moderate strength of agreement between the methods in determining IgE classes for 
      7/11 components tested. All components showed a positive correlation when 
      analysed using Spearman's rank correlation. Conclusions. Overall, we found that 
      there is good correlation between the Euroline and ImmunoCAP methods in measuring 
      IgE sensitization.
FAU - Berge, M
AU  - Berge M
AD  - Department of Otolaryngology, Orebro University Hospital, Orebro, Sweden.
FAU - Bertilsson, L
AU  - Bertilsson L
AD  - Department of Laboratory Medicine, Orebro University Hospital, Orebro, Sweden.
FAU - Hultgren, O
AU  - Hultgren O
AD  - Department Clinical Immunology and Transfusion Medicine, Faculty of Medicine and 
      Health, Orebro University, Orebro, Sweden.
FAU - Hugosson, S
AU  - Hugosson S
AD  - Deptartment of Otolaryngology, Orebro University Hospital, School of Medial 
      Sciences, Faculty of Medicine and Health, Orebro University, Orebro, Sweden.
FAU - Saber, A
AU  - Saber A
AD  - Deptartment of Otolaryngology, Orebro University Hospital, School of Medial 
      Sciences, Faculty of Medicine and Health, Orebro University, Orebro, Sweden.
LA  - eng
PT  - Journal Article
DEP - 20220114
PL  - Italy
TA  - Eur Ann Allergy Clin Immunol
JT  - European annals of allergy and clinical immunology
JID - 101466614
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Humans
MH  - *Allergens
MH  - Betula
MH  - Poaceae
MH  - *Hypersensitivity/diagnosis
MH  - Immunoblotting
MH  - Phleum
MH  - Immunoglobulin E
OTO - NOTNLM
OT  - Allergen-specific IgE
OT  - Euroline
OT  - ImmunoCAP
OT  - birch
OT  - pollen
OT  - timothy
EDAT- 2022/01/15 06:00
MHDA- 2023/03/11 06:00
CRDT- 2022/01/14 07:35
PHST- 2022/01/15 06:00 [pubmed]
PHST- 2023/03/11 06:00 [medline]
PHST- 2022/01/14 07:35 [entrez]
AID - 10.23822/EurAnnACI.1764-1489.241 [doi]
PST - ppublish
SO  - Eur Ann Allergy Clin Immunol. 2023 Mar;55(2):68-77. doi: 
      10.23822/EurAnnACI.1764-1489.241. Epub 2022 Jan 14.