PMID- 35030166 OWN - NLM STAT- MEDLINE DCOM- 20220216 LR - 20230917 IS - 1935-2735 (Electronic) IS - 1935-2727 (Print) IS - 1935-2727 (Linking) VI - 16 IP - 1 DP - 2022 Jan TI - Multiple-bead assay for the differential serodiagnosis of neglected human cestodiases: Neurocysticercosis and cystic echinococcosis. PG - e0010109 LID - 10.1371/journal.pntd.0010109 [doi] LID - e0010109 AB - BACKGROUND: Neurocysticercosis (NCC), and cystic echinococcosis (CE) are two neglected diseases caused by cestodes, co-endemic in many areas of the world. Imaging studies and serological tests are used in the diagnosis of both parasitic diseases, but cross-reactions may confound the results of the latter. The novel multiplex bead-based assay with recombinant antigens has been reported to increases the diagnostic accuracy of serological techniques. METHODOLOGY: We set-up an immunoassay based on the multiplex bead-based platform (MBA), using the rT24H (against Cysticercus cellulosae, causing cysticercosis) and r2B2t (against Echinococcus granulosus sensu lato, causing CE) recombinant antigens, for simultaneous and differential diagnosis of these infections. The antigens were tested on 356 sera from 151 patients with CE, 126 patients with NCC, and 79 individuals negative for both diseases. Specificity was calculated including sera from healthy donors, other neurological diseases and the respective NCC or CE sera counterpart. The diagnostic accuracy of this assay was compared with two commercial ELISA tests, Novalisa and Ridascreen, widely used in the routine diagnosis of cysticercosis and CE, respectively. MAIN FINDINGS: For the diagnosis of NCC, sensitivity ranged from 57.94-63.49% for the rT24H-MBA, and 40.48-46.03% for Novalisa ELISA depending on exclusion or inclusion of sera having equivocal results on ELISA from the analysis; specificities ranged from 90.87-91.30% and 70.43-76.96%, respectively. AUC values of the ROC curve were 0.783 (rT24H) and 0.619 (Novalisa) (p-value < 0.001). For the diagnosis of CE, the sensitivity of the r2B2t-MBA ranged from 68.87-69.77% and of Ridascreen ELISA from 50.00-57.62%; specificities from 92.47-92.68% and from 74.15-80.98%, respectively. AUC values were 0.717 and 0.760, respectively. CONCLUSIONS/SIGNIFICANCE: Overall, the recombinant antigens tested with the bead-based technology showed better diagnostic accuracy than the commercial assays, particularly for the diagnosis of NCC. The possibility of testing the same serum sample simultaneously for the presence of antibodies against both antigens is an added value particularly in seroprevalence studies for cysticercosis linked to control programs in endemic areas where these two parasites coexist. FAU - Hernandez-Gonzalez, Ana AU - Hernandez-Gonzalez A AD - Laboratorio de Helmintos, Centro Nacional de Microbiologia (CNM), Instituto de Salud Carlos III, Majadahonda, Madrid, Spain. FAU - Gonzalez-Bertolin, Belen AU - Gonzalez-Bertolin B AUID- ORCID: 0000-0002-8200-9193 AD - Laboratorio de Helmintos, Centro Nacional de Microbiologia (CNM), Instituto de Salud Carlos III, Majadahonda, Madrid, Spain. AD - Departamento de Microbiologia y Parasitologia, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain. FAU - Urrea, Laura AU - Urrea L AD - Laboratorio de Helmintos, Centro Nacional de Microbiologia (CNM), Instituto de Salud Carlos III, Majadahonda, Madrid, Spain. FAU - Fleury, Agnes AU - Fleury A AD - Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico (UNAM); Mexico/Instituto Nacional de Neurologia and Neurocirugia, Mexico City, Mexico. FAU - Ferrer, Elizabeth AU - Ferrer E AUID- ORCID: 0000-0002-4173-6642 AD - Instituto de Investigaciones Biomedicas "Dr. Francisco J. Triana Alonso" (BIOMED), Departamento de Parasitologia, Facultad de Ciencias de la Salud, Universidad de Carabobo Sede Aragua, Maracay, estado Aragua, Venezuela. FAU - Siles-Lucas, Mar AU - Siles-Lucas M AD - Instituto de Recursos Naturales and Agrobiologia de Salamanca, Consejo Superior de Investigaciones Cientificas (IRNASA-CSIC), Salamanca, Spain. FAU - Tamarozzi, Francesca AU - Tamarozzi F AUID- ORCID: 0000-0003-0478-3914 AD - Department of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar, Verona, Italy. FAU - Perteguer, Maria J AU - Perteguer MJ AUID- ORCID: 0000-0002-7533-5134 AD - Laboratorio de Helmintos, Centro Nacional de Microbiologia (CNM), Instituto de Salud Carlos III, Majadahonda, Madrid, Spain. AD - Departamento de Microbiologia y Parasitologia, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain. LA - eng PT - Journal Article DEP - 20220114 PL - United States TA - PLoS Negl Trop Dis JT - PLoS neglected tropical diseases JID - 101291488 RN - 0 (Antibodies, Helminth) RN - 0 (Antigens, Helminth) RN - 0 (Recombinant Proteins) SB - IM MH - Animals MH - Antibodies, Helminth/*blood MH - Antigens, Helminth/genetics/immunology MH - Echinococcosis/*diagnosis/parasitology MH - Echinococcus granulosus/*immunology MH - Enzyme-Linked Immunosorbent Assay/methods MH - Humans MH - Neglected Diseases/diagnosis/parasitology MH - Neurocysticercosis/*diagnosis/parasitology MH - Recombinant Proteins/genetics/immunology MH - Sensitivity and Specificity MH - Serologic Tests MH - Taenia solium/*immunology PMC - PMC8759687 COIS- The authors have declared that no competing interests exist. EDAT- 2022/01/15 06:00 MHDA- 2022/02/17 06:00 PMCR- 2022/01/14 CRDT- 2022/01/14 17:13 PHST- 2021/06/29 00:00 [received] PHST- 2021/12/17 00:00 [accepted] PHST- 2022/01/14 17:13 [entrez] PHST- 2022/01/15 06:00 [pubmed] PHST- 2022/02/17 06:00 [medline] PHST- 2022/01/14 00:00 [pmc-release] AID - PNTD-D-21-00940 [pii] AID - 10.1371/journal.pntd.0010109 [doi] PST - epublish SO - PLoS Negl Trop Dis. 2022 Jan 14;16(1):e0010109. doi: 10.1371/journal.pntd.0010109. eCollection 2022 Jan.