PMID- 35030723
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220118
IS  - 2576-6422 (Electronic)
IS  - 2576-6422 (Linking)
VI  - 2
IP  - 6
DP  - 2019 Jun 17
TI  - Selective Electrochemical Capture and Release of Heparin Based on 
      Amine-Functionalized Carbon/Titanium Dioxide Nanotube Arrays.
PG  - 2685-2697
LID - 10.1021/acsabm.9b00400 [doi]
AB  - Heparin (HEP) is a sulfated glycosaminoglycan that is a clinical anticoagulant 
      agent. Commercially derived from porcine intestinal mucosa, HEP is challenging to 
      separate from this complex biological mixture for additional purification. This 
      study aimed to raise the purity of isolated HEP using electrochemical potential 
      to increase its selective capture and release. We demonstrate an electrochemical 
      platform featuring an anode composed of amine-functionalized carbon/titanium 
      dioxide nanotube arrays on titanium foil (Ti/C-TNTAs-NH(2)) and a cathode made of 
      expanded graphite. Our results show that Ti and Ti/C-TNTAs control plates do not 
      adsorb HEP, even while applying an external potential to the cell. However, when 
      the Ti/C-TNTAs electrode is modified by 3 aminopropyltriethoxysilane, the 
      terminal NH(2) groups provide a high density of positive charges that serve as 
      binding sites, enabling the adsorption of HEP. This attraction is further 
      strengthened by applying an external potential to the anode. Subsequent release 
      of the HEP molecules and regeneration of the Ti/C-TNTAs-NH(2) electrode are 
      easily accomplished by applying an anodic potential to the plate, as well as by 
      increasing the concentration of NaCl in solution. This electrochemical system 
      demonstrates the good selectivity of HEP, even within a mixture of other probable 
      interfering species (e.g., bovine serum albumin and chondroitin sulfate). 
      Additionally, it maintains 90.11% of its initial electrosorption efficiency after 
      ten repeated HEP adsorption/desorption cycles, indicating this system's promising 
      stability and reusability for HEP purification.
FAU - Eskandarloo, Hamed
AU  - Eskandarloo H
AUID- ORCID: 0000-0001-7241-119X
AD  - Department of Food Science, College of Agriculture & Life Sciences, Cornell 
      University, 243 Stocking Hall, Ithaca, New York 14853, United States.
FAU - Enayati, Mojtaba
AU  - Enayati M
AUID- ORCID: 0000-0002-4408-2808
AD  - Department of Food Science, College of Agriculture & Life Sciences, Cornell 
      University, 243 Stocking Hall, Ithaca, New York 14853, United States.
FAU - Abdolmaleki, Mahmood Karimi
AU  - Abdolmaleki MK
AD  - Department of Food Science, College of Agriculture & Life Sciences, Cornell 
      University, 243 Stocking Hall, Ithaca, New York 14853, United States.
FAU - Arshadi, Mohammad
AU  - Arshadi M
AD  - Department of Food Science, College of Agriculture & Life Sciences, Cornell 
      University, 243 Stocking Hall, Ithaca, New York 14853, United States.
FAU - Abbaspourrad, Alireza
AU  - Abbaspourrad A
AUID- ORCID: 0000-0001-5617-9220
AD  - Department of Food Science, College of Agriculture & Life Sciences, Cornell 
      University, 243 Stocking Hall, Ithaca, New York 14853, United States.
LA  - eng
PT  - Journal Article
DEP - 20190524
PL  - United States
TA  - ACS Appl Bio Mater
JT  - ACS applied bio materials
JID - 101729147
SB  - IM
OTO - NOTNLM
OT  - electrosorption
OT  - functionalized nanotubes
OT  - heparin digestion
OT  - heparin recovery
OT  - selective adsorption
OT  - titanium dioxide nanotubes
EDAT- 2019/06/17 00:00
MHDA- 2019/06/17 00:01
CRDT- 2022/01/15 01:00
PHST- 2022/01/15 01:00 [entrez]
PHST- 2019/06/17 00:00 [pubmed]
PHST- 2019/06/17 00:01 [medline]
AID - 10.1021/acsabm.9b00400 [doi]
PST - ppublish
SO  - ACS Appl Bio Mater. 2019 Jun 17;2(6):2685-2697. doi: 10.1021/acsabm.9b00400. Epub 
      2019 May 24.