PMID- 35030797
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220118
IS  - 2576-6422 (Electronic)
IS  - 2576-6422 (Linking)
VI  - 2
IP  - 7
DP  - 2019 Jul 15
TI  - Ultrasensitive Electrochemiluminescence Aptasensor for Assessment of Protein 
      Heterogeneity in Small Cell Population.
PG  - 3052-3058
LID - 10.1021/acsabm.9b00352 [doi]
AB  - Development of sensitive method for assay of proteins secreted from small cell 
      population is important for the assessment of cellular heterogeneity. Here, we 
      developed an ultrasensitive electrochemiluminescence (ECL) aptasensor for 
      secreted protein in small cell population of RAW 264.7 cells for cellular 
      heterogeneity assessment. Tumor necrosis factor-alpha (TNF-alpha) was chosen as 
      target secreted protein, and specific anti-TNF-alpha aptamer was chosen as molecule 
      recognition element, while dichlorotris (1,10-phenanthroline) ruthenium(II) 
      hydrate (Ru(phen)(3)(2+)) was chosen as ECL signal compound. An ECL signal probe 
      was prepared by combining aptamer, Ru(phen)(3)(2+), and graphene oxide (GO) 
      through electrostatic interaction and pi-pi stacking interaction. An aptasensor was 
      fabricated by self-assembly specific aptamer on gold nanoparticles modified 
      electrode for the detection of TNF-alpha using sandwich assay. High sensitivity and 
      good selectivity were obtained for TNF-alpha. Parallel analysis was conducted to 
      detect TNF-alpha secreted by RAW 264.7 cells stimulated by either lipopolysaccharides 
      (LPS) or lupeol for assessing the cell heterogeneity in small cell population and 
      for estimating the intracellular signal transduction in inflammation. Compared to 
      ELISA, our ECL method offers both high sensitivity and good accuracy. The 
      distinct heterogeneity among the secreted protein in small cell population, for 
      the first time, was observed and further correlated to the cell's nature of 
      cluster growth. The developed ECL method incorporating the amplification of 
      nanomaterial and selectivity of aptamer is promising for sensitive and selective 
      analysis of biomolecules in small cell population.
FAU - Gao, Hongfang
AU  - Gao H
AD  - Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, 
      School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 
      710062, P.R. China.
FAU - Wang, Xiaofei
AU  - Wang X
AD  - Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, 
      School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 
      710062, P.R. China.
FAU - Li, Man
AU  - Li M
AD  - Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, 
      School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 
      710062, P.R. China.
FAU - Qi, Honglan
AU  - Qi H
AUID- ORCID: 0000-0002-2268-0073
AD  - Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, 
      School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 
      710062, P.R. China.
FAU - Gao, Qiang
AU  - Gao Q
AUID- ORCID: 0000-0002-0775-3414
AD  - Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, 
      School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 
      710062, P.R. China.
FAU - Zhang, Chengxiao
AU  - Zhang C
AUID- ORCID: 0000-0003-2829-5122
AD  - Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, 
      School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 
      710062, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190622
PL  - United States
TA  - ACS Appl Bio Mater
JT  - ACS applied bio materials
JID - 101729147
SB  - IM
OTO - NOTNLM
OT  - TNF-alpha
OT  - aptasensor
OT  - electrochemiluminescence
OT  - heterogeneity
OT  - small cell population
EDAT- 2019/07/15 00:00
MHDA- 2019/07/15 00:01
CRDT- 2022/01/15 01:00
PHST- 2022/01/15 01:00 [entrez]
PHST- 2019/07/15 00:00 [pubmed]
PHST- 2019/07/15 00:01 [medline]
AID - 10.1021/acsabm.9b00352 [doi]
PST - ppublish
SO  - ACS Appl Bio Mater. 2019 Jul 15;2(7):3052-3058. doi: 10.1021/acsabm.9b00352. Epub 
      2019 Jun 22.