PMID- 35036271
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230102
IS  - 2190-572X (Print)
IS  - 2190-5738 (Electronic)
IS  - 2190-5738 (Linking)
VI  - 12
IP  - 1
DP  - 2022 Jan
TI  - Duplex PCR assay for detection of chickpea chlorotic dwarf virus and peanut 
      witches' broom phytoplasma in chickpea.
PG  - 23
LID - 10.1007/s13205-021-03094-y [doi]
LID - 23
AB  - A duplex PCR assay was standardized by optimizing PCR reaction constituents and 
      cycles for the simultaneous detection of chickpea chlorotic dwarf virus (CpCDV) 
      and a peanut witches' broom (PnWB) phytoplasma associated with the chickpea stunt 
      disease. Coat protein gene and tuf gene specific primers for CpCDV and 
      phytoplasmas were used. Different concentrations of the PCR components such as 
      Taq polymerase, primers and PCR annealing temperature were standardized for the 
      identification of the two agents by a duplex PCR assay. Expected amplicons of 
      590 bp for CpCDV and 1090 bp for phytoplasmas were consistently amplified from 
      the symptomatic chickpea tissues. That resulted in equally efficient and 
      sensitive in detecting single or mixed infection of CpCDV and PnWB phytoplasma in 
      148 symptomatic chickpea stunt samples collected in two states of India. The 
      results indicate the robustness in the detection of pathogens present in chickpea 
      showing stunt disease and for theoretical use in epidemiological studies that 
      would help the appropriate disease management strategies.
CI  - (c) King Abdulaziz City for Science and Technology 2021.
FAU - Reddy, Madem Gurivi
AU  - Reddy MG
AD  - Department of Plant Pathology, S.V. Agricultural College, Acharya N.G. Ranga 
      Agricultural University, Tirupati, 517502 India. GRID: grid.472237.7. ISNI: 0000 
      0001 0559 8695
FAU - Rao, Govind Pratap
AU  - Rao GP
AUID- ORCID: 0000-0003-1057-7826
AD  - Division of Plant Pathology, Indian Agriculture Research Institute, Pusa Campus, 
      New Delhi, 110012 India.
LA  - eng
PT  - Case Reports
DEP - 20211222
PL  - Germany
TA  - 3 Biotech
JT  - 3 Biotech
JID - 101565857
PMC - PMC8695644
OTO - NOTNLM
OT  - Coat protein gene
OT  - CpCDV
OT  - Detection
OT  - PnWB phytoplasma
OT  - Tuf gene
EDAT- 2022/01/18 06:00
MHDA- 2022/01/18 06:01
PMCR- 2023/01/01
CRDT- 2022/01/17 06:03
PHST- 2021/10/23 00:00 [received]
PHST- 2021/12/11 00:00 [accepted]
PHST- 2022/01/17 06:03 [entrez]
PHST- 2022/01/18 06:00 [pubmed]
PHST- 2022/01/18 06:01 [medline]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 3094 [pii]
AID - 10.1007/s13205-021-03094-y [doi]
PST - ppublish
SO  - 3 Biotech. 2022 Jan;12(1):23. doi: 10.1007/s13205-021-03094-y. Epub 2021 Dec 22.