PMID- 35037128
OWN - NLM
STAT- MEDLINE
DCOM- 20220413
LR  - 20220531
IS  - 1432-119X (Electronic)
IS  - 0948-6143 (Linking)
VI  - 157
IP  - 4
DP  - 2022 Apr
TI  - Morphological alteration of the pancreatic islet in ovariectomized rats fed a 
      high-fat high-fructose diet.
PG  - 427-442
LID - 10.1007/s00418-021-02062-0 [doi]
AB  - Diabetes and its complications are major causes of mortality worldwide. Type 2 
      diabetes coexists with insulin resistance and beta-cell dysfunction, which are 
      aggravated by overconsumption and estrogen-deprived conditions. However, the 
      morphology of pancreatic islets in a combined condition of excessive caloric 
      intake and estrogen deficiency has never been described. Herein, we examined 
      morphological changes in the pancreatic islets of ovariectomized (OVX) rats fed a 
      high-fat high-fructose diet (HFFD) for 12 weeks. The histological changes in the 
      size and number of pancreatic islets were assessed by hematoxylin-eosin and 
      immunohistochemical staining. Enlarged pancreatic islets with fat deposition in 
      OVX rats were accompanied by whole-body insulin resistance and hyperglycemia. The 
      addition of a HFFD to OVX rats (OVX + HFFD) further aggravated insulin 
      resistance, with a substantial increase in the density of enlarged pancreatic 
      islets and fat accumulation. The augmented number of enlarged islets was 
      correlated with elevated plasma glucose and insulin levels. Intriguingly, unlike 
      the HFFD and OVX alone, the OVX + HFFD markedly expanded the area of 
      insulin-producing beta-cells and glucagon-producing alpha-cells. Importantly, enlarged 
      islets, pancreatic fat deposits, and diabetic states developing in OVX + HFFD 
      conditions were resolved by estrogen replacement. Collectively, the morphological 
      characteristics of pancreatic islets were influenced in an insulin-resistant 
      state caused by estrogen deficiency and HFFD consumption and were distinct from 
      each factor alone. A combination of estrogen deficiency with HFFD consumption 
      worsened the integrity of pancreatic islets, ultimately resulting in disease 
      progression. These findings expand our understanding of the causal relationship 
      between pancreatic morphology and diabetes development and suggest therapeutic 
      strategies.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Chansela, Piyachat
AU  - Chansela P
AD  - Department of Anatomy, Phramongkutklao College of Medicine, Bangkok, 10400, 
      Thailand.
FAU - Potip, Bubphachat
AU  - Potip B
AD  - Department of Physiology, Faculty of Science, Mahidol University, Bangkok, 10400, 
      Thailand.
FAU - Weerachayaphorn, Jittima
AU  - Weerachayaphorn J
AD  - Department of Physiology, Faculty of Science, Mahidol University, Bangkok, 10400, 
      Thailand.
FAU - Kangwanrangsan, Niwat
AU  - Kangwanrangsan N
AD  - Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok, 
      10400, Thailand.
FAU - Chukijrungroat, Natsasi
AU  - Chukijrungroat N
AD  - Department of Physiology, Faculty of Science, Mahidol University, Bangkok, 10400, 
      Thailand.
FAU - Saengsirisuwan, Vitoon
AU  - Saengsirisuwan V
AUID- ORCID: 0000-0001-7798-3680
AD  - Department of Physiology, Faculty of Science, Mahidol University, Bangkok, 10400, 
      Thailand. vitoon.sae@mahidol.ac.th.
LA  - eng
GR  - RSA-5780009/Thailand Research Fund/
GR  - RSA-6080072/Thailand Research Fund/
PT  - Journal Article
DEP - 20220117
PL  - Germany
TA  - Histochem Cell Biol
JT  - Histochemistry and cell biology
JID - 9506663
RN  - 0 (Estrogens)
RN  - 0 (Insulin)
RN  - 30237-26-4 (Fructose)
SB  - IM
MH  - Animals
MH  - *Diabetes Mellitus, Type 2/pathology
MH  - Diet, High-Fat/adverse effects
MH  - Estrogens
MH  - Female
MH  - Fructose
MH  - Insulin
MH  - *Insulin Resistance
MH  - *Islets of Langerhans/pathology
MH  - Rats
OTO - NOTNLM
OT  - Estrogen
OT  - High-fat high-fructose diet
OT  - Insulin resistance
OT  - Pancreatic beta-cells
EDAT- 2022/01/18 06:00
MHDA- 2022/04/14 06:00
CRDT- 2022/01/17 06:19
PHST- 2021/11/30 00:00 [accepted]
PHST- 2022/01/18 06:00 [pubmed]
PHST- 2022/04/14 06:00 [medline]
PHST- 2022/01/17 06:19 [entrez]
AID - 10.1007/s00418-021-02062-0 [pii]
AID - 10.1007/s00418-021-02062-0 [doi]
PST - ppublish
SO  - Histochem Cell Biol. 2022 Apr;157(4):427-442. doi: 10.1007/s00418-021-02062-0. 
      Epub 2022 Jan 17.