PMID- 35037694
OWN - NLM
STAT- MEDLINE
DCOM- 20220711
LR  - 20220711
IS  - 1699-5848 (Electronic)
IS  - 0213-3911 (Linking)
VI  - 37
IP  - 6
DP  - 2022 Jun
TI  - Circ_0044520 regulates the progression of laryngeal squamous cell carcinoma via 
      the miR-338-3p/ROR2 axis.
PG  - 513-526
LID - 10.14670/HH-18-420 [doi]
AB  - BACKGROUND: Laryngeal squamous cell carcinoma (LSCC) is the second most common 
      malignant tumor in head and neck. Circular RNA_0044520 (circ_0044520) expression 
      is increased in LSCC. However, the molecular mechanism of circ_0044520 remains 
      unknown. METHODS: The expression of circ_0044520, microRNA-338-3p (miR-338-3p) 
      and receptor tyrosine kinase-like orphan receptor 2 (ROR2) were detected by 
      quantitative real-time fluorescence polymerase chain reaction (qRT-PCR). Cell 
      viability, cell proliferation and apoptosis were detected by Cell Counting Kit-8 
      (CCK-8) assay, colony formation assay and Flow cytometry assay, respectively. 
      Western blot examined the protein levels of PCNA, Cyclin D1, Bax, Bcl-2 and ROR2 
      cells. The relationship between miR-338-3p and circ_0044520 or ROR2 was verified 
      by Dual-luciferase reporter assays. The xenotransplantation model was established 
      to study the role of circ_0044520 in vivo. RESULTS: The expression of 
      circ_0044520 and ROR2 was increased in LSCC tissues and cells, while the 
      expression of miR-338-3p was decreased. Circ_0044520 can sponge miR-338-3p, and 
      ROR2 is the target of miR-338-3p. In vitro complement experiments showed that 
      knockdown of circ_0044520 significantly inhibited malignant behavior of LSCC, 
      while co-transfection of miR-338-3p inhibitor partially up-regulated this change. 
      In addition, miR-338-3p can inhibit the proliferation of LSCC cells, while 
      co-transfection of overexpression of ROR2 can partially reverse this change. 
      Mechanically, circ_0044520 regulates ROR2 expression in LSCC cells by sponging 
      miR-338-3p. In addition, in vivo studies have shown that down-regulation of 
      circ_0044520 inhibits tumor growth. CONCLUSION: Circ_0044520 silencing plays 
      anti-proliferation and pro-apoptosis roles in LSCC cells by regulating the 
      miR-338-3p/ROR2 axis, suggesting that the circ_0044520/miR-338-3p/ROR2 axis may 
      be a potential regulatory mechanism for the treatment of LSCC.
FAU - Yang, Huijun
AU  - Yang H
AD  - Department of Otolaryngology, First Affiliated Hospital of China Medical 
      University, Shenyang City, Liaoning, China. yanghuijun2004@163.com.
FAU - Yu, Gang
AU  - Yu G
AD  - Department of Otolaryngology, First Affiliated Hospital of China Medical 
      University, Shenyang City, Liaoning, China.
FAU - Wang, Yan
AU  - Wang Y
AD  - Department of Otolaryngology, First Affiliated Hospital of China Medical 
      University, Shenyang City, Liaoning, China.
FAU - Guo, Xing
AU  - Guo X
AD  - Department of Otolaryngology, First Affiliated Hospital of China Medical 
      University, Shenyang City, Liaoning, China.
LA  - eng
PT  - Journal Article
DEP - 20220117
PL  - Spain
TA  - Histol Histopathol
JT  - Histology and histopathology
JID - 8609357
RN  - 0 (MIRN338 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - EC 2.7.10.1 (ROR2 protein, human)
RN  - EC 2.7.10.1 (Receptor Tyrosine Kinase-like Orphan Receptors)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - *Head and Neck Neoplasms
MH  - Humans
MH  - *MicroRNAs/genetics/metabolism
MH  - RNA, Circular/genetics
MH  - Receptor Tyrosine Kinase-like Orphan Receptors/metabolism
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
EDAT- 2022/01/18 06:00
MHDA- 2022/07/12 06:00
CRDT- 2022/01/17 09:39
PHST- 2022/01/18 06:00 [pubmed]
PHST- 2022/07/12 06:00 [medline]
PHST- 2022/01/17 09:39 [entrez]
AID - HH-18-420 [pii]
AID - 10.14670/HH-18-420 [doi]
PST - ppublish
SO  - Histol Histopathol. 2022 Jun;37(6):513-526. doi: 10.14670/HH-18-420. Epub 2022 
      Jan 17.