PMID- 35038081
OWN - NLM
STAT- MEDLINE
DCOM- 20220407
LR  - 20220531
IS  - 1573-7365 (Electronic)
IS  - 0885-7490 (Linking)
VI  - 37
IP  - 3
DP  - 2022 Mar
TI  - EIF4A3-induced circCCNB1 (hsa_circ_0001495) promotes glioma progression by 
      elevating CCND1 through interacting miR-516b-5p and HuR.
PG  - 819-833
LID - 10.1007/s11011-021-00899-x [doi]
AB  - To explore the functions of circRNA cyclin B1 (circCCNB1) in glioma and its 
      possible mechanisms. The expression of circCCNB1, eukaryotic translation 
      initiation factor 4A3 (EIF4A3), cyclin D1 (CCND1) and miR-516b-5p was determined 
      by qRT-PCR, western blot or immunohistochemistry (IHC) assay. The feature of 
      circCCNB1 was analyzed by Actinomycin D (ActD), RNase R and subcellular fraction 
      assays. The molecule relationships were analyzed by RIP, dual-luciferase reporter 
      and RNA pull-down assays. CCK-8, EdU and colony formation assays were performed 
      to analyze cell proliferation. Flow cytometry analysis was executed to estimate 
      the cell cycle. Murine xenograft model assay was used for the role of circCCNB1 
      in vivo. CircCCNB1 was overexpressed in glioma tissues and cells. EIF4A3 
      positively regulated circCCNB1 expression. CircCCNB1 knockdown repressed glioma 
      cell proliferation and cell cycle process in vitro and blocked tumor growth in 
      vivo. CircCCNB1 knockdown reduced CCND1 expression in glioma cells and CCND1 
      overexpression bated the effect of circCCNB1 knockdown on glioma cell growth. 
      CircCCNB1 interacted with HuR to elevate CCND1 expression. miR-516b-5p could 
      interact with circCCNB1 and CCND1. CircCCNB1 regulated glioma cell progression 
      and CCND1 expression by miR-516b-5p and HuR. CircCCNB1 aggravated glioma cell 
      growth by elevating CCND1 through targeting miR-516b-5p and HuR.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Li, Xiaoli
AU  - Li X
AD  - Department of Neurology, Renmin Hospital, Hubei University of Medicine, No. 39, 
      Chaoyang Middle Road, Maojian District, Shiyan, 442000, China.
FAU - Wang, Chengmou
AU  - Wang C
AD  - Department of Neurology, Renmin Hospital, Hubei University of Medicine, No. 39, 
      Chaoyang Middle Road, Maojian District, Shiyan, 442000, China.
FAU - Chen, Guanghui
AU  - Chen G
AD  - Department of Neurology, Renmin Hospital, Hubei University of Medicine, No. 39, 
      Chaoyang Middle Road, Maojian District, Shiyan, 442000, China.
FAU - Zou, Wenqin
AU  - Zou W
AD  - Department of Neurology, Renmin Hospital, Hubei University of Medicine, No. 39, 
      Chaoyang Middle Road, Maojian District, Shiyan, 442000, China.
FAU - Deng, Yanqing
AU  - Deng Y
AD  - Department of Neurology, Renmin Hospital, Hubei University of Medicine, No. 39, 
      Chaoyang Middle Road, Maojian District, Shiyan, 442000, China.
FAU - Zhou, Faming
AU  - Zhou F
AD  - Department of Neurology, Renmin Hospital, Hubei University of Medicine, No. 39, 
      Chaoyang Middle Road, Maojian District, Shiyan, 442000, China. fmzhou002@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220117
PL  - United States
TA  - Metab Brain Dis
JT  - Metabolic brain disease
JID - 8610370
RN  - 0 (CCND1 protein, human)
RN  - 0 (Cyclin B1)
RN  - 0 (ELAV-Like Protein 1)
RN  - 0 (ELAVL1 protein, human)
RN  - 0 (MIRN516 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 136601-57-5 (Cyclin D1)
RN  - EC 2.7.7.- (Eukaryotic Initiation Factor-4A)
RN  - EC 3.6.1.- (EIF4A3 protein, human)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Cyclin B1
MH  - Cyclin D1/genetics/metabolism
MH  - DEAD-box RNA Helicases/metabolism
MH  - ELAV-Like Protein 1
MH  - Eukaryotic Initiation Factor-4A/genetics/metabolism
MH  - *Glioma/genetics
MH  - Humans
MH  - Mice
MH  - *MicroRNAs/genetics/metabolism
MH  - RNA, Circular/genetics
OTO - NOTNLM
OT  - CCND1
OT  - Glioma
OT  - HuR
OT  - circCCNB1
OT  - miR-516b-5p
EDAT- 2022/01/18 06:00
MHDA- 2022/04/08 06:00
CRDT- 2022/01/17 12:25
PHST- 2021/08/06 00:00 [received]
PHST- 2021/12/26 00:00 [accepted]
PHST- 2022/01/18 06:00 [pubmed]
PHST- 2022/04/08 06:00 [medline]
PHST- 2022/01/17 12:25 [entrez]
AID - 10.1007/s11011-021-00899-x [pii]
AID - 10.1007/s11011-021-00899-x [doi]
PST - ppublish
SO  - Metab Brain Dis. 2022 Mar;37(3):819-833. doi: 10.1007/s11011-021-00899-x. Epub 
      2022 Jan 17.