PMID- 35039938 OWN - NLM STAT- MEDLINE DCOM- 20221026 LR - 20221026 IS - 1591-9528 (Electronic) IS - 1591-8890 (Linking) VI - 22 IP - 4 DP - 2022 Nov TI - Association between serum miR-221-3p and intravenous immunoglobulin resistance in children with Kawasaki disease. PG - 621-628 LID - 10.1007/s10238-021-00776-y [doi] AB - OBJECTIVES: Intravenous immunoglobulin (IVIG) resistance was a major cause of coronary artery lesions in children with Kawasaki disease (KD). However, the cause of IVIG resistance in KD remains unknown. miR-221-3p has been confirmed involved in cardiovascular diseases and rheumatoid arthritis. The purpose of this study was to investigate the association between miR-221-3p and IVIG resistance in children with KD. METHODS: Fifty-five KD patients and 29 healthy controls (HCs) were enrolled in this study. KD patients were divided into group of sensitive to IVIG (IVIG-response, n = 42) and group of resistant to IVIG (IVIG-resistance, n = 13), group of 10 KD patients with coronary artery lesions (CALs, KD-CALs) and group of 10 sex- and age-matched KD patients without CALs (KD-NCALs). Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect the levels of miR-221-3p. RESULTS: Compared with the HCs group, miR-221-3p were significantly increased in the KD group (p < 0.05), and the IVIG-resistance group had higher levels of miR-221-3p than those in the IVIG-response group (p < 0.05). CRP (C-reactive protein), PCT (procalcitonin), NLR (neutrophil-lymphocyte ratio) were positively correlated with miR-221-3p in KD patients. In addition, the group of IVIG resistance had a higher level of Kobayashi Score (p < 0.001). The receiver operating characteristic curve showed that miR-221-3p had a better value for diagnosis IVIG resistance in children with KD than Kobayashi Score with the AUC of 0.811 (95% CI, 0.672-0.951), 0.793 (95% CI, 0.618-0.968), respectively. Additionally, miR-221-3p was elevated (p < 0.05) and showed an AUC value of 0.83 (95% CI, 0.648-1.000, p < 0.05) for the prediction of the complication of coronary artery abnormalities in the group of KD with CALs. CONCLUSIONS: miR-221-3p might be involved in the pathogenesis of KD and IVIG resistance and miR-221-3p can be used as a new potential biomarker to predict IVIG resistance in children with KD. CI - (c) 2021. The Author(s), under exclusive licence to Springer Nature Switzerland AG. FAU - Jing, Fengchuan AU - Jing F AD - Department of Cardiovascular Medicine, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. AD - Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. AD - National Clinical Research Center for Child Health and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. AD - China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. FAU - Weng, Haobo AU - Weng H AD - Department of Cardiovascular Medicine, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. AD - Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. AD - National Clinical Research Center for Child Health and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. AD - China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. FAU - Pei, Qiongfei AU - Pei Q AD - Department of Cardiovascular Medicine, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. AD - Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. AD - National Clinical Research Center for Child Health and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. AD - China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. FAU - Zhang, Jing AU - Zhang J AD - Department of Cardiovascular Medicine, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. AD - Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. AD - National Clinical Research Center for Child Health and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. AD - China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. FAU - Liu, Ruixi AU - Liu R AD - Department of Cardiovascular Medicine, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. 270815992@qq.com. AD - Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. 270815992@qq.com. AD - National Clinical Research Center for Child Health and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. 270815992@qq.com. AD - China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. 270815992@qq.com. AD - Chongqing Key Laboratory of Pediatrics, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. 270815992@qq.com. FAU - Yi, Qijian AU - Yi Q AUID- ORCID: 0000-0002-4461-3904 AD - Department of Cardiovascular Medicine, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. qjyi2003@aliyun.com. AD - Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. qjyi2003@aliyun.com. AD - National Clinical Research Center for Child Health and Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. qjyi2003@aliyun.com. AD - China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. qjyi2003@aliyun.com. AD - Chongqing Key Laboratory of Pediatrics, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China. qjyi2003@aliyun.com. LA - eng PT - Journal Article DEP - 20220118 PL - Italy TA - Clin Exp Med JT - Clinical and experimental medicine JID - 100973405 RN - 0 (Biomarkers) RN - 9007-41-4 (C-Reactive Protein) RN - 0 (Immunoglobulins, Intravenous) RN - 0 (MicroRNAs) RN - 0 (MIRN221 microRNA, human) RN - 0 (Procalcitonin) SB - IM MH - Child MH - Humans MH - Biomarkers MH - C-Reactive Protein/metabolism MH - Immunoglobulins, Intravenous/therapeutic use MH - *MicroRNAs/genetics MH - *Mucocutaneous Lymph Node Syndrome/diagnosis/drug therapy MH - Procalcitonin MH - Retrospective Studies OTO - NOTNLM OT - Intravenous immunoglobulin resistance OT - Kawasaki disease OT - microRNA-221 EDAT- 2022/01/19 06:00 MHDA- 2022/10/26 06:00 CRDT- 2022/01/18 06:07 PHST- 2021/07/21 00:00 [received] PHST- 2021/11/06 00:00 [accepted] PHST- 2022/01/19 06:00 [pubmed] PHST- 2022/10/26 06:00 [medline] PHST- 2022/01/18 06:07 [entrez] AID - 10.1007/s10238-021-00776-y [pii] AID - 10.1007/s10238-021-00776-y [doi] PST - ppublish SO - Clin Exp Med. 2022 Nov;22(4):621-628. doi: 10.1007/s10238-021-00776-y. Epub 2022 Jan 18.