PMID- 35040975
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220218
IS  - 2662-6810 (Print)
IS  - 2052-7276 (Electronic)
IS  - 2052-7276 (Linking)
VI  - 9
DP  - 2022 Jan 5
TI  - Illustration of the variation in the content of flavanone rutinosides in various 
      citrus germplasms from genetic and enzymatic perspectives.
LID - 10.1093/hr/uhab017 [doi]
LID - uhab017
AB  - In citrus, 1,6-rhamnosytransferase (1,6RhaT) and 1,2-rhamnosytransferase 
      (1,2RhaT) catalyze flavanone-7-O-glucosides to form nonbitter flavanone 
      rutinosides (FRs) and bitter flavanone neohesperidosides (FNs), respectively. As 
      revealed in this study of fruit peels from 36 citrus accessions, FRs varied from 
      undetectable levels in pummelo and kumquat to being the dominant flavonoids in 
      sweet orange and loose-skin mandarins. Furthermore, a previously annotated 
      full-length 1,6RhaT-like gene was identified as another 1,6RhaT-encoding gene by 
      in vitro experiments. In total, 28 alleles of full-length 1,6RhaTs were isolated 
      and classified into A, B and C types with only type A alleles encoding a 
      functional protein. Coincidently, only the accessions that contained FRs harbored 
      type A alleles, as was further verified in two F1 hybrid populations. Moreover, 
      the inferior substrate conversion efficiency of 1,6RhaTs in comparison with that 
      of 1,2RhaT in vitro might partly explain the lower proportions of FRs to total 
      flavanone disaccharides in citrus hybrids harboring both functional 
      rhamnosyltransferases. Our findings provide a better understanding of FR content 
      variations among citrus and are meaningful for a mechanistic illustration of 
      citrus flavonoid metabolism and fruit quality improvement practices.
CI  - (c) The Author(s) 2022. Published by Oxford University Press on behalf of Nanjing 
      Agricultural University.
FAU - Li, Wenyun
AU  - Li W
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, No.1 Shizishan 
      Street, Hongshan District, Wuhan 430070, China.
AD  - Guizhou Fruit Institute, Guizhou Academy of Agricultural Sciences, No.1 Jinnong 
      Road, Huaxi District, Guiyang 550006, China.
FAU - Li, Gu
AU  - Li G
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, No.1 Shizishan 
      Street, Hongshan District, Wuhan 430070, China.
FAU - Yuan, Ziyu
AU  - Yuan Z
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, No.1 Shizishan 
      Street, Hongshan District, Wuhan 430070, China.
FAU - Li, Mingyue
AU  - Li M
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, No.1 Shizishan 
      Street, Hongshan District, Wuhan 430070, China.
FAU - Deng, Xiuxin
AU  - Deng X
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, No.1 Shizishan 
      Street, Hongshan District, Wuhan 430070, China.
FAU - Tan, Meilian
AU  - Tan M
AD  - The Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, 
      No.2 Xudong Second Road, Wuchang District, Wuhan 430062, China.
FAU - Ma, Yuhua
AU  - Ma Y
AD  - Guizhou Fruit Institute, Guizhou Academy of Agricultural Sciences, No.1 Jinnong 
      Road, Huaxi District, Guiyang 550006, China.
FAU - Chen, Jiajing
AU  - Chen J
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, No.1 Shizishan 
      Street, Hongshan District, Wuhan 430070, China.
FAU - Xu, Juan
AU  - Xu J
AD  - Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of 
      Horticulture and Forestry, Huazhong Agricultural University, No.1 Shizishan 
      Street, Hongshan District, Wuhan 430070, China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Hortic Res
JT  - Horticulture research
JID - 101655540
PMC - PMC8788359
EDAT- 2022/01/19 06:00
MHDA- 2022/01/19 06:01
PMCR- 2022/01/18
CRDT- 2022/01/18 12:33
PHST- 2021/03/11 00:00 [received]
PHST- 2021/07/16 00:00 [revised]
PHST- 2021/08/30 00:00 [accepted]
PHST- 2022/01/18 12:33 [entrez]
PHST- 2022/01/19 06:00 [pubmed]
PHST- 2022/01/19 06:01 [medline]
PHST- 2022/01/18 00:00 [pmc-release]
AID - 6510704 [pii]
AID - uhab017 [pii]
AID - 10.1093/hr/uhab017 [doi]
PST - ppublish
SO  - Hortic Res. 2022 Jan 5;9:uhab017. doi: 10.1093/hr/uhab017.