PMID- 35044600
OWN - NLM
STAT- MEDLINE
DCOM- 20220426
LR  - 20220531
IS  - 1708-0428 (Electronic)
IS  - 0960-8923 (Linking)
VI  - 32
IP  - 4
DP  - 2022 Apr
TI  - Rise in Postprandial GLP-1 Levels After Roux-en-Y Gastric Bypass: Involvement of 
      the Vagus Nerve-Spleen Anti-inflammatory Axis in Type 2 Diabetic Rats.
PG  - 1077-1085
LID - 10.1007/s11695-021-05877-x [doi]
AB  - PURPOSE: The mechanism underlying postprandial glucagon-like peptide-1 (GLP-1) 
      changes after metabolic surgery remains mostly unclarified. This investigation 
      aimed to address whether the vagus nerve-spleen anti-inflammatory axis is 
      involved in the rise in postprandial GLP-1 levels in type 2 diabetes mellitus 
      (T2DM) rats following metabolic surgery. MATERIALS AND METHODS: T2DM rat model 
      was established with a high-fat diet and a low dose of streptozotocin and 
      subjected to Roux-en-Y gastric bypass (RYGB) and splenic denervation. A 
      mixed-meal tolerance test for postprandial GLP-1 response was performed. TNF-alpha in 
      the plasma, spleen, and ileum was measured by ELISA, and alpha 7 nicotinic 
      acetylcholine receptor (alpha7nAChR) expression in the spleen was analyzed by Western 
      blot. RESULTS: Postprandial GLP-1 improvement by RYGB was accompanied by the 
      reduction of TNF-alpha levels in spleen and ileum and up-regulation of splenic 
      alpha7nAChR in T2DM rats. Splenic denervation abrogates a rise in postprandial GLP-1 
      levels in response to the mixed-meal challenge, along with higher TNF-alpha levels in 
      spleen and ileum and down-regulation of splenicalpha7nAChR, compared with denervated 
      sham rats. CONCLUSION: Our results reveal that the vagus nerve-spleen 
      anti-inflammatory axis mediates the rise of postprandial GLP-1 response after 
      RYGB through lowering TNF-alpha contents in the intestinal tissue in T2DM rats.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Xiao, Yan
AU  - Xiao Y
AD  - Department of Day Surgery Center, Zhuzhou Central Hospital, Zhuzhou, 412000, 
      China.
FAU - Tan, Chang
AU  - Tan C
AD  - Department of Gynecology, Zhuzhou Central Hospital, Zhuzhou, 412000, China.
FAU - Nie, Xiaoya
AU  - Nie X
AD  - Department of General Medicine, Zhuzhou Central Hospital, No. 116 Changjiang 
      Road, Zhuzhou, 412000, China.
FAU - Li, Baifeng
AU  - Li B
AD  - Department of Day Surgery Center, Zhuzhou Central Hospital, Zhuzhou, 412000, 
      China.
FAU - You, Miao
AU  - You M
AD  - Department of Day Surgery Center, Zhuzhou Central Hospital, Zhuzhou, 412000, 
      China.
FAU - Lan, Yunyun
AU  - Lan Y
AD  - Department of Intensive Care Unit, Zhuzhou Central Hospital, No.116 Changjiang 
      Road, Zhuzhou, 412000, China. lanyunyunzz@outlook.com.
FAU - Tang, Liang
AU  - Tang L
AUID- ORCID: 0000-0002-9603-1535
AD  - Department of General Medicine, Zhuzhou Central Hospital, No. 116 Changjiang 
      Road, Zhuzhou, 412000, China. cntangliang@outlook.com.
LA  - eng
PT  - Journal Article
DEP - 20220119
PL  - United States
TA  - Obes Surg
JT  - Obesity surgery
JID - 9106714
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Blood Glucose)
RN  - 0 (Insulin)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (alpha7 Nicotinic Acetylcholine Receptor)
RN  - 89750-14-1 (Glucagon-Like Peptide 1)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents
MH  - Blood Glucose/metabolism
MH  - *Diabetes Mellitus, Experimental/metabolism
MH  - *Diabetes Mellitus, Type 2
MH  - *Gastric Bypass/methods
MH  - Glucagon-Like Peptide 1
MH  - Humans
MH  - Insulin
MH  - *Obesity, Morbid/surgery
MH  - Rats
MH  - Spleen/chemistry/metabolism/surgery
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Vagus Nerve/surgery
MH  - alpha7 Nicotinic Acetylcholine Receptor
OTO - NOTNLM
OT  - Glucagon-like peptide-1
OT  - Roux-en-Y gastric bypass
OT  - Spleen
OT  - Type 2 diabetes mellitus
OT  - Vagus nerve anti-inflammatory pathway
EDAT- 2022/01/20 06:00
MHDA- 2022/04/27 06:00
CRDT- 2022/01/19 12:32
PHST- 2021/05/11 00:00 [received]
PHST- 2021/12/30 00:00 [accepted]
PHST- 2021/12/30 00:00 [revised]
PHST- 2022/01/20 06:00 [pubmed]
PHST- 2022/04/27 06:00 [medline]
PHST- 2022/01/19 12:32 [entrez]
AID - 10.1007/s11695-021-05877-x [pii]
AID - 10.1007/s11695-021-05877-x [doi]
PST - ppublish
SO  - Obes Surg. 2022 Apr;32(4):1077-1085. doi: 10.1007/s11695-021-05877-x. Epub 2022 
      Jan 19.