PMID- 35045921
OWN - NLM
STAT- MEDLINE
DCOM- 20220408
LR  - 20220408
IS  - 0031-7144 (Print)
IS  - 0031-7144 (Linking)
VI  - 77
IP  - 1
DP  - 2022 Jan 3
TI  - Changes of BKCA current and vascular reactivity in vascular smooth muscle of 
      thoracic aorta and mesenteric artery in type 2 diabetic rats.
PG  - 21-26
LID - 10.1691/ph.2022.1740 [doi]
AB  - The relationship between large conductance calcium activated potassium channel 
      (BK(Ca)) and vascular lesions in type 2 diabetes mellitus (T2DM) was investigated 
      by observing vascular reactivity of thoracic aorta and mesenteric artery and the 
      current changes of BK(Ca) in vascular smooth muscle cells (VSMCs). The thoracic 
      aorta and mesenteric artery of T2DM rats were isolated, the whole cell perforated 
      patch clamp experiment and single channel patch clamp experiment of acute enzyme 
      separation of thoracic aorta and mesenteric artery smooth muscle cells were 
      performed to measure the membrane capacitance and the amplitude of macro current. 
      And the vascular ring experiment was performed to observe the change of 
      relaxation percentage. The results showed that the amplitude of BK(Ca) current in 
      vascular smooth muscle cells of diabetic rats was higher than that of the control 
      group. The channel current had outward rectifying characteristics. BK(Ca) is 
      related to vascular reactivity and smooth muscle relaxation in T2DM rats. The 
      opening probability of BK(Ca) in VSMCs of diabetic rats was significantly 
      increased. This study suggests that BK(Ca) may be a new target for diabetic 
      vascular disease.
FAU - Si, Yuankai
AU  - Si Y
AD  - Department of Pharmacy, Southwest Medical University, Sichuan, China; Department 
      of Pharmacy, Affiliated Hospital of Southwest Medical University, Sichuan, China.
FAU - Wu, Zhigui
AU  - Wu Z
AD  - Department of Pharmacy, Affiliated Hospital of Southwest Medical University, 
      Sichuan, China.
FAU - Gu, Li
AU  - Gu L
AD  - Department of Pharmacy, Southwest Medical University, Sichuan, China.
FAU - Li, Hua
AU  - Li H
AD  - Department of Pharmacy, Southwest Medical University, Sichuan, China.
FAU - Ge, Pei
AU  - Ge P
AD  - Department of Pharmacy, Affiliated Hospital of Southwest Medical University, 
      Sichuan, China.
FAU - Yin, Wenxian
AU  - Yin W
AD  - Department of Pharmacy, Affiliated Hospital of Traditional Chinese Medicine 
      Southwest Medical University, Sichuan, China.
FAU - Chen, Meijuan
AU  - Chen M
AD  - Department of Pharmacy, Southwest Medical University, Sichuan, China;, Email: 
      chenmeijuan1969@sina.com.
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Pharmazie
JT  - Die Pharmazie
JID - 9800766
SB  - IM
MH  - Animals
MH  - Aorta, Thoracic
MH  - *Diabetes Mellitus, Experimental/metabolism
MH  - *Diabetes Mellitus, Type 2
MH  - Mesenteric Arteries/metabolism
MH  - Muscle, Smooth, Vascular
MH  - Myocytes, Smooth Muscle
MH  - Rats
EDAT- 2022/01/21 06:00
MHDA- 2022/04/09 06:00
CRDT- 2022/01/20 05:39
PHST- 2022/01/20 05:39 [entrez]
PHST- 2022/01/21 06:00 [pubmed]
PHST- 2022/04/09 06:00 [medline]
AID - 10.1691/ph.2022.1740 [doi]
PST - ppublish
SO  - Pharmazie. 2022 Jan 3;77(1):21-26. doi: 10.1691/ph.2022.1740.