PMID- 35046942 OWN - NLM STAT- MEDLINE DCOM- 20220124 LR - 20220124 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 12 DP - 2021 TI - Single-Cell Analysis Reveals the Immune Characteristics of Myeloid Cells and Memory T Cells in Recovered COVID-19 Patients With Different Severities. PG - 781432 LID - 10.3389/fimmu.2021.781432 [doi] LID - 781432 AB - Despite many studies on the immune characteristics of Coronavirus disease 2019 (COVID-19) patients in the progression stage, a detailed understanding of pertinent immune cells in recovered patients is lacking. We performed single-cell RNA sequencing on samples from recovered COVID-19 patients and healthy controls. We created a comprehensive immune landscape with more than 260,000 peripheral blood mononuclear cells (PBMCs) from 41 samples by integrating our dataset with previously reported datasets, which included samples collected between 27 and 47 days after symptom onset. According to our large-scale single-cell analysis, recovered patients, who had severe symptoms (severe/critical recovered), still exhibited peripheral immune disorders 1-2 months after symptom onset. Specifically, in these severe/critical recovered patients, human leukocyte antigen (HLA) class II and antigen processing pathways were downregulated in both CD14 monocytes and dendritic cells compared to healthy controls, while the proportion of CD14 monocytes increased. These may lead to the downregulation of T-cell differentiation pathways in memory T cells. However, in the mild/moderate recovered patients, the proportion of plasmacytoid dendritic cells increased compared to healthy controls, accompanied by the upregulation of HLA-DRA and HLA-DRB1 in both CD14 monocytes and dendritic cells. In addition, T-cell differentiation regulation and memory T cell-related genes FOS, JUN, CD69, CXCR4, and CD83 were upregulated in the mild/moderate recovered patients. Further, the immunoglobulin heavy chain V3-21 (IGHV3-21) gene segment was preferred in B-cell immune repertoires in severe/critical recovered patients. Collectively, we provide a large-scale single-cell atlas of the peripheral immune response in recovered COVID-19 patients. CI - Copyright (c) 2022 Li, Garg, Jia, Liao, Yuan, Li, Wu, Wu, Bi, George, Papatheodorou, Brazma, Luo, Fang, Miao and Shu. FAU - Li, Xu AU - Li X AD - School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China. FAU - Garg, Manik AU - Garg M AD - European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Hinxton, Cambridge, United Kingdom. FAU - Jia, Tingting AU - Jia T AD - School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China. FAU - Liao, Qijun AU - Liao Q AD - School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China. FAU - Yuan, Lifang AU - Yuan L AD - School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China. FAU - Li, Mao AU - Li M AD - School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China. FAU - Wu, Zhengyu AU - Wu Z AD - School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China. FAU - Wu, Weihua AU - Wu W AD - Major Infectious Disease Control Key Laboratory, Shenzhen Center for Disease Control and Prevention, Shenzhen, China. FAU - Bi, Yalan AU - Bi Y AD - European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Hinxton, Cambridge, United Kingdom. FAU - George, Nancy AU - George N AD - European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Hinxton, Cambridge, United Kingdom. FAU - Papatheodorou, Irene AU - Papatheodorou I AD - European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Hinxton, Cambridge, United Kingdom. FAU - Brazma, Alvis AU - Brazma A AD - European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Hinxton, Cambridge, United Kingdom. FAU - Luo, Huanle AU - Luo H AD - School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China. FAU - Fang, Shisong AU - Fang S AD - Major Infectious Disease Control Key Laboratory, Shenzhen Center for Disease Control and Prevention, Shenzhen, China. FAU - Miao, Zhichao AU - Miao Z AD - European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Hinxton, Cambridge, United Kingdom. AD - Translational Research Institute of Brain and Brain-Like Intelligence and Department of Anesthesiology, Shanghai Fourth People's Hospital Affiliated to Tongji University School of Medicine, Shanghai, China. FAU - Shu, Yuelong AU - Shu Y AD - School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen, China. LA - eng GR - 108437/Z/15/Z/WT_/Wellcome Trust/United Kingdom PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220103 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 SB - IM MH - COVID-19/genetics/*immunology MH - Dendritic Cells/*immunology MH - Female MH - Humans MH - Male MH - Memory T Cells/*immunology MH - Monocytes/*immunology MH - *RNA-Seq MH - SARS-CoV-2/*immunology MH - *Single-Cell Analysis PMC - PMC8762286 OTO - NOTNLM OT - HLA class II OT - disease severity OT - memory T cells OT - myeloid cells OT - recovered COVID-19 patients COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2022/01/21 06:00 MHDA- 2022/01/27 06:00 PMCR- 2022/01/03 CRDT- 2022/01/20 06:02 PHST- 2021/09/22 00:00 [received] PHST- 2021/12/03 00:00 [accepted] PHST- 2022/01/20 06:02 [entrez] PHST- 2022/01/21 06:00 [pubmed] PHST- 2022/01/27 06:00 [medline] PHST- 2022/01/03 00:00 [pmc-release] AID - 10.3389/fimmu.2021.781432 [doi] PST - epublish SO - Front Immunol. 2022 Jan 3;12:781432. doi: 10.3389/fimmu.2021.781432. eCollection 2021.