PMID- 35049071
OWN - NLM
STAT- MEDLINE
DCOM- 20220428
LR  - 20220428
IS  - 1399-3054 (Electronic)
IS  - 0031-9317 (Linking)
VI  - 174
IP  - 2
DP  - 2022 Mar
TI  - OsCyp2-P, an auxin-responsive cyclophilin, regulates Ca(2+) calmodulin 
      interaction for an ion-mediated stress response in rice.
PG  - e13631
LID - 10.1111/ppl.13631 [doi]
AB  - OsCYP2-P is an active cyclophilin (having peptidyl-prolyl cis/trans-isomerase 
      activity, PPIase) isolated from the wild rice Pokkali having a natural capacity 
      to grow and yield seeds in coastal saline regions of India. Transcript abundance 
      analysis in rice seedlings showed the gene is inducible by multiple stresses, 
      including salinity, drought, high temperature, and heavy metals. To dissect the 
      role of OsCYP2-P gene in stress response, we raised overexpression (OE) and 
      knockdown (KD) transgenic rice plants with >2-3 folds higher and approximately 
      2-fold lower PPIase activity, respectively. Plants overexpressing this gene had 
      more favorable physiological and biochemical parameters (K(+) /Na(+) ratio, 
      electrolytic leakage, membrane damage, antioxidant enzymes) than wild type, and 
      the reverse was observed in plants that were knocked down for this gene. We 
      propose that OsCYP2-P contributes to stress tolerance via maintenance of ion 
      homeostasis and thus prevents toxic cellular ion buildup and membrane damage. OE 
      plants were found to have a higher harvest index and higher number of filled 
      grains under salinity and drought stress than wild type. OsCYP2-P interacts with 
      calmodulin, indicating it functions via the Ca-CaM pathway. Compared to the WT, 
      the germinating OE seeds exhibited a substantially higher auxin level, and this 
      hormone was below the detection limits in the WT and KD lines. These observations 
      strongly indicate that OsCyp2-P affects the signaling and transport of auxin in 
      rice.
CI  - (c) 2022 Scandinavian Plant Physiology Society.
FAU - Roy, Suchismita
AU  - Roy S
AD  - School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
FAU - Mishra, Manjari
AU  - Mishra M
AD  - School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
FAU - Kaur, Gundeep
AU  - Kaur G
AD  - Department of Biotechnology, Guru Nanak Dev University, Amritsar, India.
FAU - Singh, Supreet
AU  - Singh S
AD  - Department of Biotechnology, Guru Nanak Dev University, Amritsar, India.
FAU - Rawat, Nishtha
AU  - Rawat N
AD  - School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
FAU - Singh, Prabhjeet
AU  - Singh P
AD  - Department of Biotechnology, Guru Nanak Dev University, Amritsar, India.
FAU - Singla-Pareek, Sneh L
AU  - Singla-Pareek SL
AUID- ORCID: 0000-0002-0521-2622
AD  - Plant Stress Biology, International Centre for Genetic Engineering and 
      Biotechnology (ICGEB), New Delhi, India.
FAU - Pareek, Ashwani
AU  - Pareek A
AUID- ORCID: 0000-0002-2923-0681
AD  - School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
AD  - National Agri-Food Biotechnology Institute (NABI), Mohali, India.
LA  - eng
GR  - Ministry of Science and Technology/
GR  - Department of Biotechnology/
PT  - Journal Article
PL  - Denmark
TA  - Physiol Plant
JT  - Physiologia plantarum
JID - 1256322
RN  - 0 (Calmodulin)
RN  - 0 (Indoleacetic Acids)
RN  - 0 (Plant Proteins)
RN  - EC 5.2.1.- (Cyclophilins)
SB  - IM
MH  - Calmodulin/genetics/metabolism
MH  - Cyclophilins/genetics/metabolism
MH  - Droughts
MH  - Gene Expression Regulation, Plant
MH  - Indoleacetic Acids/metabolism
MH  - *Oryza/metabolism
MH  - Plant Proteins/genetics/metabolism
MH  - Plants, Genetically Modified/genetics
MH  - Stress, Physiological/genetics
EDAT- 2022/01/21 06:00
MHDA- 2022/04/29 06:00
CRDT- 2022/01/20 09:09
PHST- 2021/12/23 00:00 [revised]
PHST- 2021/12/02 00:00 [received]
PHST- 2022/01/14 00:00 [accepted]
PHST- 2022/01/21 06:00 [pubmed]
PHST- 2022/04/29 06:00 [medline]
PHST- 2022/01/20 09:09 [entrez]
AID - 10.1111/ppl.13631 [doi]
PST - ppublish
SO  - Physiol Plant. 2022 Mar;174(2):e13631. doi: 10.1111/ppl.13631.