PMID- 35049602
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220124
IS  - 2304-6767 (Electronic)
IS  - 2304-6767 (Linking)
VI  - 10
IP  - 1
DP  - 2021 Dec 30
TI  - SATB2 and MDM2 Immunoexpression and Diagnostic Role in Primary Osteosarcomas of 
      the Jaw.
LID - 10.3390/dj10010004 [doi]
LID - 4
AB  - Primary osteosarcomas of the jaw (OSJ) are rare, accounting for 6% of all 
      osteosarcomas. This study aims to determine the value of SATB2 and MDM2 
      immunohistochemistry (IHC) in differentiating OSJ from other jawbone mimickers, 
      such as benign fibro-osseous lesions (BFOLs) of the jaw or Ewing sarcoma of the 
      jaw. Certain subsets of osteosarcoma harbor a supernumerary ring and/or giant 
      marker chromosomes with amplification of the 12q13-15 region, including the 
      murine double-minute type 2 (MDM2) and cyclin-dependent kinase 4 (CDK4) genes. 
      Special AT-rich sequence-binding protein 2 (SATB2) is an immunophenotypic marker 
      for osteoblastic differentiation. Cases of OSJ, BFOLs (ossifying fibroma and 
      fibrous dysplasia) of the jaw, and Ewing sarcoma of the jaw were retrieved from 
      the Departments of Oral Pathology and Oral Medicine, Faculty of Dentistry, 
      Obafemi Awolowo University and Lagos State University College of Medicine, 
      Nigeria. All OSJ retrieved showed histologic features of high-grade osteosarcoma. 
      IHC for SATB2 (clone EP281) and MDM2 (clone IF2), as well as fluorescence in situ 
      hybridization (FISH) for MDM2 amplification, were performed on all cases. SATB2 
      was expressed in a strong intensity and diffuse staining pattern in all cases (11 
      OSJ, including a small-cell variant, 7 ossifying fibromas, and 5 fibrous 
      dysplasias) except in Ewing sarcoma, where it was negative in neoplastic cells. 
      MDM2 was expressed in a weak to moderate intensity and scattered focal to limited 
      diffuse staining pattern in 27% (3/11) of cases of OSJ and negative in all BFOLs 
      and the Ewing sarcoma. MDM2 amplification was negative by FISH in interpretable 
      cases. In conclusion, the three cases of high-grade OSJs that expressed MDM2 may 
      have undergone transformation from a low-grade osteosarcoma (LGOS). SATB2 is not 
      a dependable diagnostic marker to differentiate OSJ from BFOLs of the jaw; 
      however, it could serve as a valuable diagnostic marker in differentiating the 
      small-cell variant of OSJ from Ewing sarcoma of the jaw, while MDM2 may be a 
      useful diagnostic marker in differentiating OSJ from BFOLs of the jaw, especially 
      in the case of an LGOS or high-grade transformed osteosarcoma.
FAU - Owosho, Adepitan A
AU  - Owosho AA
AD  - Missouri School of Dentistry and Oral Health, A.T. Still University, Kirksville, 
      MO 63501, USA.
FAU - Ladeji, Adeola M
AU  - Ladeji AM
AD  - Department of Oral Pathology and Oral Medicine, Faculty of Dentistry, Lagos State 
      University, Lagos 101233, Nigeria.
FAU - Adesina, Olufunlola M
AU  - Adesina OM
AUID- ORCID: 0000-0003-3687-4255
AD  - Department of Oral Medicine and Oral Pathology, Faculty of Dentistry, Obafemi 
      Awolowo University, Ile-Ife 220282, Nigeria.
FAU - Adebiyi, Kehinde E
AU  - Adebiyi KE
AD  - Department of Oral Pathology and Oral Medicine, Faculty of Dentistry, Lagos State 
      University, Lagos 101233, Nigeria.
FAU - Olajide, Mofoluwaso A
AU  - Olajide MA
AD  - Department of Oral Pathology and Oral Medicine, Faculty of Dentistry, Lagos State 
      University, Lagos 101233, Nigeria.
FAU - Okunade, Toluwaniyin
AU  - Okunade T
AD  - Department of Oral Medicine and Oral Pathology, Faculty of Dentistry, Obafemi 
      Awolowo University, Ile-Ife 220282, Nigeria.
FAU - Palmer, Jacob
AU  - Palmer J
AD  - Missouri School of Dentistry and Oral Health, A.T. Still University, Kirksville, 
      MO 63501, USA.
FAU - Kehinde, Temitope
AU  - Kehinde T
AD  - Department of Pathology, Anatomy and Laboratory Medicine, West Virginia 
      University, Morgantown, WV 26506, USA.
FAU - Vos, Jeffrey A
AU  - Vos JA
AD  - Department of Pathology, Anatomy and Laboratory Medicine, West Virginia 
      University, Morgantown, WV 26506, USA.
FAU - Cole, Grayson
AU  - Cole G
AD  - Department of Diagnostic Sciences, School of Dental Medicine, University of 
      Pittsburgh, Pittsburgh, PA 15213, USA.
FAU - Summersgill, Kurt F
AU  - Summersgill KF
AD  - Department of Diagnostic Sciences, School of Dental Medicine, University of 
      Pittsburgh, Pittsburgh, PA 15213, USA.
LA  - eng
PT  - Journal Article
DEP - 20211230
PL  - Switzerland
TA  - Dent J (Basel)
JT  - Dentistry journal
JID - 101716125
PMC - PMC8775091
OTO - NOTNLM
OT  - MDM2 amplification
OT  - fibrous dysplasia
OT  - immunohistochemistry
OT  - jaw tumors
OT  - ossifying fibroma
COIS- The authors declare no conflict of interest.
EDAT- 2022/01/21 06:00
MHDA- 2022/01/21 06:01
PMCR- 2021/12/30
CRDT- 2022/01/20 12:25
PHST- 2021/11/08 00:00 [received]
PHST- 2021/12/17 00:00 [revised]
PHST- 2021/12/20 00:00 [accepted]
PHST- 2022/01/20 12:25 [entrez]
PHST- 2022/01/21 06:00 [pubmed]
PHST- 2022/01/21 06:01 [medline]
PHST- 2021/12/30 00:00 [pmc-release]
AID - dj10010004 [pii]
AID - dentistry-10-00004 [pii]
AID - 10.3390/dj10010004 [doi]
PST - epublish
SO  - Dent J (Basel). 2021 Dec 30;10(1):4. doi: 10.3390/dj10010004.