PMID- 35051563 OWN - NLM STAT- MEDLINE DCOM- 20220314 LR - 20220314 IS - 1095-9947 (Electronic) IS - 1050-4648 (Linking) VI - 121 DP - 2022 Feb TI - Changes in immune genes expression, immune response, digestive enzymes -antioxidant status, and growth of catla (Catla catla) fed with Astragalus polysaccharides against edwardsiellosis disease. PG - 418-436 LID - S1050-4648(22)00031-6 [pii] LID - 10.1016/j.fsi.2022.01.022 [doi] AB - The effect of four level of Astragalus polysaccharides (APs) supplementation diets, (CD: control diet and three experiment diet (E), EA: 100 mg kg(-1) APs; EB: 200 mg kg-1 APs; EC: 300 mg kg(-1) APs) on growth, changes in haemato-biochemical parameters and metabolic-digestive enzymes, enhancement of antioxidant activity, innate-adaptive immune response, and cytokine gene expression were studied in catla (Catla catla) against Edwardsiella tarda. The healthy and challenged groups fed the CD displayed no mortality, while fish fed EA or EC revealed 10% mortality, but the mortality was only 5% in diet EB. Fish fed diet EB and EC revealed significantly better growth rates and high RBC count during the experimental period. Albumin and globulin levels were significant improved when fish were fed the diet EB and EC from weeks 6-8. The superoxide dismutase (SOD) was significant ameliorated by EB feeding from weeks 4-8. In contrast, serum myeloperoxidase (MPO), catalase (CAT), malondialdehyde (MDA)/lipid peroxidation (LPO), glutathione peroxidase (GPx), respiratory burst activity (RBA), bactericidal action (BCA), serum lysozyme activity (SLA), nitric oxide synthase (NOS), head kidney leukocytes response proliferation (HKLP), hemolytic action (HLA), hydrogen peroxides (H(2)O(2)), and immunoglobulin (Ig) were significantly improved from week 6-8. Groups fed the APs enriched diets had significant ameliorated interleukin (IL)-1beta and interferon (IFN)-gamma mRNA expression after 6 and 8 weeks of feeding. However, IL-10 and major histocompatibility complex (MHC)-1 mRNA expressions were significant enhanced in catla fed all APs diets on week 8. APs enriched diets revealed significant improved tumor necrosis factor (TNF)-alpha and TNF receptor-associated factor-6 (TRAF6) mRNA expression on week 4, but toll-like receptor-2 (TLR2) and TLR4 mRNA expression were significant enhanced by diet EB and EC after weeks 6 and 8. Similarly, the lysozyme (Lyz)-C and Lyz-G mRNA levels in the head kidney (HK) increased by APs feeding on weeks 6 and 8, whereas the EB diet, the expression of nucleotide binding oligomerization domain-1 (NOD1) was significantly improved on weeks 6 and 8, but NOD2 mRNA expression was only significant enhanced after 8 weeks of diet EB. By feeding healthy catla and E. tarda challenged fish fed diet EB, resulted in significantly increased growth, haemato-biochemical indices, metabolic-digestive enzymes, antioxidant activities, innate-adaptive immune responses, and cytokine gene expression mainly between 6 and 8 weeks. CI - Crown Copyright (c) 2022. Published by Elsevier Ltd. All rights reserved. FAU - Harikrishnan, Ramasamy AU - Harikrishnan R AD - Department of Zoology, Pachaiyappa's College for Men, Kanchipuram, 631 501, Tamil Nadu, India. Electronic address: rhari123@yahoo.com. FAU - Devi, Gunapathy AU - Devi G AD - Department of Zoology, Nehru Memorial College, Puthanampatti, 621 007, Tamil Nadu, India. FAU - Doan, Hien Van AU - Doan HV AD - Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand; Innoviative Agriculture Research Centre, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand. Electronic address: hien.d@cmu.ac.th. FAU - Tapingkae, Wanaporn AU - Tapingkae W AD - Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand. FAU - Balasundaram, Chellam AU - Balasundaram C AD - Department of Herbal and Environmental Science, Tamil University, Thanjavur, 613 005, Tamil Nadu, India. FAU - Arockiaraj, Jesu AU - Arockiaraj J AD - Foundation for Aquaculture Innovations and Technology Transfer (FAITT), Thoraipakkam, 600 097, Chennai, Tamil Nadu, India; Department of Biotechnology, College of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, 603 203, Chennai, Tamil Nadu, India. FAU - Ringo, Einar AU - Ringo E AD - Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries and Economics, UiT, The Arctic University of Norway, Tromso, 9037, Norway. LA - eng PT - Journal Article DEP - 20220118 PL - England TA - Fish Shellfish Immunol JT - Fish & shellfish immunology JID - 9505220 RN - 0 (Antioxidants) RN - 0 (Cytokines) RN - 0 (Polysaccharides) RN - 0 (RNA, Messenger) RN - BBX060AN9V (Hydrogen Peroxide) RN - EC 3.2.1.17 (Muramidase) SB - IM MH - Animal Feed/analysis MH - Animals MH - Antioxidants/metabolism MH - Astragalus Plant/chemistry MH - *Cyprinidae/growth & development/immunology/microbiology MH - Cytokines MH - *Diet/veterinary MH - Dietary Supplements MH - Edwardsiella tarda/pathogenicity MH - Enterobacteriaceae Infections/immunology/*veterinary MH - *Fish Diseases MH - Hydrogen Peroxide MH - Immunity MH - Muramidase MH - Polysaccharides/*administration & dosage MH - RNA, Messenger OTO - NOTNLM OT - Astragalus polysaccharides (APs) OT - Catla catla OT - Chemokine genes OT - Edwardsiella tarda OT - Innate-adaptive immune response EDAT- 2022/01/21 06:00 MHDA- 2022/03/15 06:00 CRDT- 2022/01/20 20:16 PHST- 2021/09/08 00:00 [received] PHST- 2021/12/12 00:00 [revised] PHST- 2022/01/15 00:00 [accepted] PHST- 2022/01/21 06:00 [pubmed] PHST- 2022/03/15 06:00 [medline] PHST- 2022/01/20 20:16 [entrez] AID - S1050-4648(22)00031-6 [pii] AID - 10.1016/j.fsi.2022.01.022 [doi] PST - ppublish SO - Fish Shellfish Immunol. 2022 Feb;121:418-436. doi: 10.1016/j.fsi.2022.01.022. Epub 2022 Jan 18.