PMID- 35051693
OWN - NLM
STAT- MEDLINE
DCOM- 20220217
LR  - 20220217
IS  - 1874-9968 (Electronic)
IS  - 0041-624X (Linking)
VI  - 121
DP  - 2022 Apr
TI  - Low-intensity pulsed ultrasound enhances bone marrow-derived stem cells-based 
      periodontal regenerative therapies.
PG  - 106678
LID - S0041-624X(21)00293-6 [pii]
LID - 10.1016/j.ultras.2021.106678 [doi]
AB  - BACKGROUND: Alveolar bone loss is one of the most common consequence for 
      periodontitis, which is a major obstacle in periodontal regeneration. Bone marrow 
      stromal cells (BMSCs) have shown significant promise in the treatment of various 
      disease, which also contribute to the natural bone repair process. Low-intensity 
      pulsed ultrasound (LIPUS) is a therapeutic ultrasound used in our previous 
      studies to promotes alveolar bone regeneration. In addition, LIPUS was found to 
      be a promising method to enhance mesenchymal stromal cell-based therapies. In the 
      current study, we have investigated the effects of LIPUS combined with BMSCs 
      therapies on BMSCs homing and its potential to promote alveolar bone 
      regeneration. METHODS: BMSCs were isolated from rat and characterized by 
      multilineages differentiation assay. Then these cells were labeled with 
      luciferase and green fluorescent protein (GFP) by lentivirus in vitro. 
      Periodontal bone defect was made on the mesial area of the maxillary first molar 
      in rats. A total of 1 x 10(6) Luc-GFP labeled BMSCs were injected into rat tail 
      vein. Bioluminescence imaging was utilized to track BMSCs in vivo. The rats were 
      sacrificed eight weeks after surgery and the samples were harvested. 
      Micro-computed tomography (Micro-CT) was performed to evaluate alveolar bone 
      regeneration. Paraffin sections were made and subject to hematoxylin-eosin 
      staining, masson staining and immunohistochemistry staining. RESULTS: BMSCs 
      display a fibroblast-like morphology and can differentiate into adipocytes or 
      osteoblasts under appropriate condition. The transfected BMSCs are strongly 
      positive for GFP express. Bioluminescence imaging showed that most of BMSCs were 
      trapped in the lung. A small portion BMSCs were homed to the alveolar bone defect 
      area in BMSCs group, while more cells were observed in BMSCs/LIPUS group compare 
      to other groups on day 3 and 7. Micro-CT results showed that BMSCs/LIPUS group 
      resulted in more new bone formation than other groups. Immunohistochemical 
      results showed higher expression of COL-I and osteopontin in BMSCs/LIPUS group 
      compared with the other groups. CONCLUSIONS: These results suggested that LIPUS 
      can enhance BMSCs-based periodontal alveolar bone regeneration. This study 
      provides new insights into how LIPUS might provide therapeutic benefits by 
      promoting BMSCs homing.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Wang, Yunji
AU  - Wang Y
AD  - College of Stomatology, Chongqing Medical University, Chongqing, China; Chongqing 
      Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China; 
      Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher 
      Education, Chongqing, China.
FAU - Li, Jie
AU  - Li J
AD  - College of Stomatology, Chongqing Medical University, Chongqing, China; Chongqing 
      Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China; 
      Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher 
      Education, Chongqing, China.
FAU - Zhou, Jianpin
AU  - Zhou J
AD  - College of Stomatology, Chongqing Medical University, Chongqing, China; Chongqing 
      Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China; 
      Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher 
      Education, Chongqing, China.
FAU - Qiu, Ye
AU  - Qiu Y
AD  - College of Stomatology, Chongqing Medical University, Chongqing, China; Chongqing 
      Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China; 
      Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher 
      Education, Chongqing, China. Electronic address: 500694@hospital.cqmu.edu.cn.
FAU - Song, Jinlin
AU  - Song J
AD  - College of Stomatology, Chongqing Medical University, Chongqing, China; Chongqing 
      Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China; 
      Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher 
      Education, Chongqing, China. Electronic address: songjinlin@hospital.cqmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220104
PL  - Netherlands
TA  - Ultrasonics
JT  - Ultrasonics
JID - 0050452
SB  - IM
MH  - Alveolar Bone Loss/*therapy
MH  - Animals
MH  - Bone Regeneration/*radiation effects
MH  - Cell- and Tissue-Based Therapy/*methods
MH  - Guided Tissue Regeneration/*methods
MH  - Mesenchymal Stem Cells/*radiation effects
MH  - Rats
MH  - *Ultrasonic Waves
OTO - NOTNLM
OT  - Alveolar bone
OT  - Bone marrow stromal cells
OT  - Cell homing
OT  - Low-intensity pulsed ultrasound
EDAT- 2022/01/21 06:00
MHDA- 2022/02/19 06:00
CRDT- 2022/01/20 20:20
PHST- 2021/06/23 00:00 [received]
PHST- 2021/12/17 00:00 [revised]
PHST- 2021/12/30 00:00 [accepted]
PHST- 2022/01/21 06:00 [pubmed]
PHST- 2022/02/19 06:00 [medline]
PHST- 2022/01/20 20:20 [entrez]
AID - S0041-624X(21)00293-6 [pii]
AID - 10.1016/j.ultras.2021.106678 [doi]
PST - ppublish
SO  - Ultrasonics. 2022 Apr;121:106678. doi: 10.1016/j.ultras.2021.106678. Epub 2022 
      Jan 4.