PMID- 35052826
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220128
IS  - 2227-9059 (Print)
IS  - 2227-9059 (Electronic)
IS  - 2227-9059 (Linking)
VI  - 10
IP  - 1
DP  - 2022 Jan 11
TI  - Distinctive Roles of YAP and TAZ in Human Endothelial Progenitor Cells Growth and 
      Functions.
LID - 10.3390/biomedicines10010147 [doi]
LID - 147
AB  - The hippo signaling pathway plays an essential role in controlling organ size and 
      balancing tissue homeostasis. Its two main effectors, yes-associated protein 
      (YAP) and WW domain-containing transcription regulator 1, WWTR1 or TAZ, have also 
      been shown to regulate endothelial cell functions and angiogenesis. In this 
      study, the functions of YAP and TAZ in human endothelial progenitor cells (EPCs) 
      were investigated by a loss-of-function study using CRISPR/Cas9-mediated gene 
      knockdown (KD). Depletion of either YAP or TAZ reduced EPC survival and impaired 
      many of their critical functions, including migration, invasion, 
      vessel-formation, and expression of pro-angiogenic genes. Notably, TAZ-KD EPCs 
      exhibited more severe phenotypes in comparison to YAP-KD EPCs. Moreover, the 
      conditioned medium derived from TAZ-KD EPCs reduced the survivability of human 
      lung cancer cells and increased their sensitivity to chemotherapeutic agents. The 
      overexpression of either wild-type or constitutively active TAZ rescued the 
      impaired phenotypes of TAZ-KD EPCs and restored the expression of pro-angiogenic 
      genes in those EPCs. In summary, we demonstrate the crucial role of Hippo 
      signaling components, YAP and TAZ, in controlling several aspects of EPC 
      functions that can potentially be used as a drug target to enhance EPC functions 
      in patients.
FAU - Klaihmon, Phatchanat
AU  - Klaihmon P
AD  - Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj 
      Hospital, Mahidol University, Bangkok 10700, Thailand.
FAU - Lorthongpanich, Chanchao
AU  - Lorthongpanich C
AD  - Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj 
      Hospital, Mahidol University, Bangkok 10700, Thailand.
FAU - Kheolamai, Pakpoom
AU  - Kheolamai P
AD  - Center of Excellence in Stem Cell Research and Innovation, Faculty of Medicine, 
      Thammasat University, Pathum Thani 12120, Thailand.
FAU - Luanpitpong, Sudjit
AU  - Luanpitpong S
AUID- ORCID: 0000-0002-1639-4935
AD  - Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj 
      Hospital, Mahidol University, Bangkok 10700, Thailand.
FAU - Issaragrisil, Surapol
AU  - Issaragrisil S
AD  - Siriraj Center of Excellence for Stem Cell Research, Faculty of Medicine Siriraj 
      Hospital, Mahidol University, Bangkok 10700, Thailand.
LA  - eng
GR  - N41A640154/National Research Council of Thailand/
GR  - N41A640122/National Research Council of Thailand/
GR  - D003276/Siriraj Foundation for Stem Cell Research/
PT  - Journal Article
DEP - 20220111
PL  - Switzerland
TA  - Biomedicines
JT  - Biomedicines
JID - 101691304
PMC - PMC8773510
OTO - NOTNLM
OT  - YAP/TAZ
OT  - endothelial progenitor cells
OT  - hippo signaling pathway
COIS- The authors declare no potential conflicts of interest.
EDAT- 2022/01/22 06:00
MHDA- 2022/01/22 06:01
PMCR- 2022/01/11
CRDT- 2022/01/21 01:03
PHST- 2021/11/04 00:00 [received]
PHST- 2022/01/04 00:00 [revised]
PHST- 2022/01/04 00:00 [accepted]
PHST- 2022/01/21 01:03 [entrez]
PHST- 2022/01/22 06:00 [pubmed]
PHST- 2022/01/22 06:01 [medline]
PHST- 2022/01/11 00:00 [pmc-release]
AID - biomedicines10010147 [pii]
AID - biomedicines-10-00147 [pii]
AID - 10.3390/biomedicines10010147 [doi]
PST - epublish
SO  - Biomedicines. 2022 Jan 11;10(1):147. doi: 10.3390/biomedicines10010147.