PMID- 35057930
OWN - NLM
STAT- MEDLINE
DCOM- 20220124
LR  - 20220124
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 1192
DP  - 2022 Feb 1
TI  - Selection and characterization of DNA aptamers for highly selective recognition 
      of the major allergen of olive pollen Ole e 1.
PG  - 339334
LID - S0003-2670(21)01160-0 [pii]
LID - 10.1016/j.aca.2021.339334 [doi]
AB  - In this study, single-stranded DNA aptamers with binding affinity to Ole e 1, the 
      major allergen of olive pollen, were selected using systematic evolution of 
      ligands by exponential enrichment (SELEX) method. Binding of the aptamers was 
      firstly established by enzyme-linked oligonucleotide assay (ELONA) and 
      aptaprecipitation assays. Additionally, aptamer-modified monolithic capillary 
      chromatography was used in order to evaluate the recognition of this allergenic 
      protein against other non-target proteins. The results indicated that AptOle1#6 
      was the aptamer that provided the highest affinity for Ole e 1. The selected 
      aptamer showed good selective recognition of this protein, being not able to 
      retain other non-target proteins (HSA, cyt c, and other pollen protein such as 
      Ole e 9). The feasibility of the affinity monolithic column was demonstrated by 
      selective recognition of Ole e 1 in an allergy skin test. The stability and 
      reproducibility of this monolithic column was suitable, with relative standard 
      deviations (RSDs) in retention times and peak area values of 7.8 and 9.3%, 
      respectively (column-to-column reproducibility). This is the first study that 
      describes the design of an efficient DNA aptamer for this relevant allergen.
CI  - Copyright (c) 2021 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Vergara-Barberan, Maria
AU  - Vergara-Barberan M
AD  - Department of Analytical Chemistry, University of Valencia, C. Doctor Moliner 50, 
      E-46100, Burjassot, Valencia, Spain.
FAU - Lerma-Garcia, Maria Jesus
AU  - Lerma-Garcia MJ
AD  - Department of Analytical Chemistry, University of Valencia, C. Doctor Moliner 50, 
      E-46100, Burjassot, Valencia, Spain.
FAU - Simo-Alfonso, Ernesto F
AU  - Simo-Alfonso EF
AD  - Department of Analytical Chemistry, University of Valencia, C. Doctor Moliner 50, 
      E-46100, Burjassot, Valencia, Spain.
FAU - Garcia-Hernandez, Marta
AU  - Garcia-Hernandez M
AD  - Grupo de Aptameros. Departamento de Bioquimica-Investigacion, Hospital 
      Universitario Ramon y Cajal (IRYCIS), Carretera de Colmenar Viejo Km.9.100, 
      CP-28034, Madrid, Spain.
FAU - Martin, M Elena
AU  - Martin ME
AD  - Grupo de Aptameros. Departamento de Bioquimica-Investigacion, Hospital 
      Universitario Ramon y Cajal (IRYCIS), Carretera de Colmenar Viejo Km.9.100, 
      CP-28034, Madrid, Spain.
FAU - Garcia-Sacristan, Ana
AU  - Garcia-Sacristan A
AD  - Aptus Biotech SL, Av. Cardenal Herrera Oria 298, CP-28035, Madrid, Spain.
FAU - Gonzalez, Victor M
AU  - Gonzalez VM
AD  - Grupo de Aptameros. Departamento de Bioquimica-Investigacion, Hospital 
      Universitario Ramon y Cajal (IRYCIS), Carretera de Colmenar Viejo Km.9.100, 
      CP-28034, Madrid, Spain; Aptus Biotech SL, Av. Cardenal Herrera Oria 298, 
      CP-28035, Madrid, Spain.
FAU - Herrero-Martinez, Jose Manuel
AU  - Herrero-Martinez JM
AD  - Department of Analytical Chemistry, University of Valencia, C. Doctor Moliner 50, 
      E-46100, Burjassot, Valencia, Spain. Electronic address: jmherrer@uv.es.
LA  - eng
PT  - Journal Article
DEP - 20211202
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Allergens)
RN  - 0 (Aptamers, Nucleotide)
SB  - IM
MH  - Allergens
MH  - *Aptamers, Nucleotide
MH  - *Olea
MH  - Pollen
MH  - Reproducibility of Results
OTO - NOTNLM
OT  - Affinity-based monolith
OT  - Allergen
OT  - Aptamer
OT  - Enzyme-linked oligonucleotide assay
OT  - Ole e 1
OT  - Olive pollen
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/01/22 06:00
MHDA- 2022/01/27 06:00
CRDT- 2022/01/21 05:48
PHST- 2021/07/29 00:00 [received]
PHST- 2021/11/23 00:00 [revised]
PHST- 2021/11/25 00:00 [accepted]
PHST- 2022/01/21 05:48 [entrez]
PHST- 2022/01/22 06:00 [pubmed]
PHST- 2022/01/27 06:00 [medline]
AID - S0003-2670(21)01160-0 [pii]
AID - 10.1016/j.aca.2021.339334 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2022 Feb 1;1192:339334. doi: 10.1016/j.aca.2021.339334. Epub 2021 
      Dec 2.