PMID- 35060474
OWN - NLM
STAT- MEDLINE
DCOM- 20220214
LR  - 20220214
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Linking)
VI  - 103
IP  - 1
DP  - 2022 Jan
TI  - miR-505 inhibits replication of Borna disease virus 1 via inhibition of 
      HMGB1-mediated autophagy.
LID - 10.1099/jgv.0.001713 [doi]
AB  - Borna disease virus 1 (BoDV-1) is a highly neurotropic RNA virus which was 
      recently demonstrated to cause deadly human encephalitis. Viruses can modulate 
      microRNA expression, in turn modulating cellular immune responses and regulating 
      viral replication. A previous study indicated that BoDV-1 infection 
      down-regulated the expression of miR-505 in rats. However, the underlying 
      mechanism of miR-505 during BoDV-1 infection remains unknown. In this study, we 
      found that miR-505 can inhibit autophagy activation by down-regulating the 
      expression of its target gene HMGB1, and ultimately inhibit the replication of 
      BoDV-1. Specifically, we found that the expression of miR-505 was significantly 
      down-regulated in rat primary neurons stably infected with BoDV-1. Overexpression 
      of miR-505 can inhibit the replication of BoDV-1 in cells. Bioinformatics 
      analysis and dual luciferase reporter gene detection confirmed that during BoDV-1 
      infection, the high-mobility group protein B1 (HMGB1) that mediates autophagy is 
      the direct target gene of miR-505. The expression of HMGB1 was up-regulated after 
      BoDV-1 infection, and overexpression of miR-505 could inhibit the expression of 
      HMGB1. Autophagy-related detection found that after infection with BoDV-1, the 
      expression of autophagy-related proteins and autophagy-related marker LC3 in 
      neuronal cells was significantly up-regulated. Autophagy flow experiments and 
      transmission electron microscopy also further confirmed that BoDV-1 infection 
      activated HMGB1-mediated autophagy. Further regulating the expression of miR-505 
      found that overexpression of miR-505 significantly inhibited HMGB1-mediated 
      autophagy. The discovery of this mechanism may provide new ideas and directions 
      for the prevention and treatment of BoDV-1 infection in the future.
FAU - Guo, Yujie
AU  - Guo Y
AD  - Department of Neurology, Yongchuan Hospital of Chongqing Medical University, 
      Chongqing, PR China.
AD  - NHC Key Laboratory of Diagnosis and Treatment on Brain Functional Diseases, The 
      First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China.
FAU - Xu, Xiaoyan
AU  - Xu X
AD  - NHC Key Laboratory of Diagnosis and Treatment on Brain Functional Diseases, The 
      First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China.
AD  - Department of Pathology, College of Basic Medicine, Chongqing Medical University, 
      Chongqing, PR China.
FAU - Tang, Tian
AU  - Tang T
AD  - Department of Laboratory Medicine, Jintang First People's Hospital, West China 
      Hospital Sichuan University JinTang Hospital, Chengdu, Sichuan, PR China.
FAU - Sun, Lin
AU  - Sun L
AD  - Department of Anaesthesia and Pain, The First People's Hospital of Chongqing 
      Liangjiang New Area, Chongqing, PR China.
FAU - Zhang, Xiong
AU  - Zhang X
AD  - Department of Neurology, the Second Affiliated Hospital of Chongqing Medical 
      University, Chongqing, PR China.
FAU - Shen, Xia
AU  - Shen X
AD  - NHC Key Laboratory of Diagnosis and Treatment on Brain Functional Diseases, The 
      First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China.
AD  - Department of Neurology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, PR China.
FAU - Li, Dan
AU  - Li D
AD  - Department of Pathology, College of Basic Medicine, Chongqing Medical University, 
      Chongqing, PR China.
FAU - Wang, Lixin
AU  - Wang L
AD  - Department of Pathology, College of Basic Medicine, Chongqing Medical University, 
      Chongqing, PR China.
FAU - Zhao, Libo
AU  - Zhao L
AD  - Department of Neurology, Yongchuan Hospital of Chongqing Medical University, 
      Chongqing, PR China.
FAU - Xie, Peng
AU  - Xie P
AD  - NHC Key Laboratory of Diagnosis and Treatment on Brain Functional Diseases, The 
      First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China.
AD  - Department of Neurology, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (HMGB1 Protein)
RN  - 0 (HMGB1 protein, human)
RN  - 0 (MIRN505 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Autophagy
MH  - Borna Disease/*genetics/metabolism/*virology
MH  - Borna disease virus/*physiology
MH  - HEK293 Cells
MH  - HMGB1 Protein/*genetics/metabolism
MH  - Humans
MH  - MicroRNAs/*genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Virus Replication
OTO - NOTNLM
OT  - BoDV-1
OT  - HMGB1
OT  - autophagy
OT  - miR-505
OT  - viral replication
EDAT- 2022/01/22 06:00
MHDA- 2022/02/15 06:00
CRDT- 2022/01/21 08:44
PHST- 2022/01/21 08:44 [entrez]
PHST- 2022/01/22 06:00 [pubmed]
PHST- 2022/02/15 06:00 [medline]
AID - 10.1099/jgv.0.001713 [doi]
PST - ppublish
SO  - J Gen Virol. 2022 Jan;103(1). doi: 10.1099/jgv.0.001713.