PMID- 35064359
OWN - NLM
STAT- MEDLINE
DCOM- 20220915
LR  - 20220922
IS  - 1573-4927 (Electronic)
IS  - 0006-2928 (Linking)
VI  - 60
IP  - 5
DP  - 2022 Oct
TI  - CircRNA CORO1C Regulates miR-654-3p/USP7 Axis to Mediate Laryngeal Squamous Cell 
      Carcinoma Progression.
PG  - 1615-1629
LID - 10.1007/s10528-021-10169-1 [doi]
AB  - Circular RNAs have attracted the attention in the research on laryngeal squamous 
      cell carcinoma (LSCC) development. But the function and mechanism of circRNA 
      coronin-1C (circ_CORO1C) in LSCC progression are largely unknown. Circ_CORO1C, 
      microRNA (miR)-654-3p, and ubiquitin-specific protease 7 (USP7) levels in LSCC 
      tissues and cells were determined. Quantitative reverse transcription polymerase 
      chain reaction and western blotting assays were conducted to determine the mRNA 
      and protein levels of genes. Functional assays were further investigated by 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), EdU 
      staining, flow cytometry, transwell and xenograft assays. The binding 
      relationship was examined by dual-luciferase reporter assay. Circ_CORO1C 
      expression was elevated in LSCC samples and cells. circ_CORO1C silencing 
      constrained cell proliferation and promoted apoptosis via reducing cell 
      proliferation, inducing cell cycle arrest, inhibiting epithelial-mesenchymal 
      transition, and promoting apoptosis, as well as restraining cell migration and 
      invasion. USP7 is highly expressed in LSCC tissues and cells, and its expression 
      was suppressed by circ_CORO1C silencing. Besides, the up-regulation of USP7 
      attenuated the influence of circ_CORO1C silencing on LSCC cell progression. Both 
      circ_CORO1C and USP7 could bind to miR-654-3p. circ_CORO1C regulated USP7 
      expression by modulating miR-654-3p. miR-654-3p knockdown mitigated the influence 
      of circ_CORO1C silence on LSCC cell progression. Furthermore, circ_CORO1C 
      silencing reduced tumor growth in vivo. In conclusion, circ_CORO1C is highly 
      expressed in LSCC tissues and cells, and circ_CORO1C silencing repressed LSCC 
      progression via regulating miR-654-3p/USP7 axis.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Zhang, Jianbin
AU  - Zhang J
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Xiangyang Central 
      Hospital, Affiliated Hospital of Hubei University of Arts and Science, No. 136 
      Jingzhou Street, Xiangcheng District, Xiangyang, 441021, China.
FAU - Li, He
AU  - Li H
AUID- ORCID: 0000-0002-2983-467X
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Xiangyang Central 
      Hospital, Affiliated Hospital of Hubei University of Arts and Science, No. 136 
      Jingzhou Street, Xiangcheng District, Xiangyang, 441021, China. nhvqfb@163.com.
FAU - Li, Jiangping
AU  - Li J
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Xiangyang Central 
      Hospital, Affiliated Hospital of Hubei University of Arts and Science, No. 136 
      Jingzhou Street, Xiangcheng District, Xiangyang, 441021, China.
FAU - Ke, Saixiong
AU  - Ke S
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Xiangyang Central 
      Hospital, Affiliated Hospital of Hubei University of Arts and Science, No. 136 
      Jingzhou Street, Xiangcheng District, Xiangyang, 441021, China.
LA  - eng
PT  - Journal Article
DEP - 20220122
PL  - United States
TA  - Biochem Genet
JT  - Biochemical genetics
JID - 0126611
RN  - 0 (MIRN654 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Microfilament Proteins)
RN  - 0 (RNA, Circular)
RN  - 145420-64-0 (coronin proteins)
RN  - EC 3.4.19.12 (USP7 protein, human)
RN  - EC 3.4.19.12 (Ubiquitin-Specific Peptidase 7)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - *Laryngeal Neoplasms/genetics
MH  - *MicroRNAs/genetics
MH  - Microfilament Proteins/genetics
MH  - *RNA, Circular/genetics
MH  - *Squamous Cell Carcinoma of Head and Neck/genetics
MH  - *Ubiquitin-Specific Peptidase 7/genetics
OTO - NOTNLM
OT  - Laryngeal squamous cell carcinoma
OT  - USP7
OT  - Ubiquitination
OT  - circ_CORO1C
OT  - miR-654-3p
EDAT- 2022/01/23 06:00
MHDA- 2022/09/16 06:00
CRDT- 2022/01/22 05:45
PHST- 2021/07/29 00:00 [received]
PHST- 2021/12/06 00:00 [accepted]
PHST- 2022/01/23 06:00 [pubmed]
PHST- 2022/09/16 06:00 [medline]
PHST- 2022/01/22 05:45 [entrez]
AID - 10.1007/s10528-021-10169-1 [pii]
AID - 10.1007/s10528-021-10169-1 [doi]
PST - ppublish
SO  - Biochem Genet. 2022 Oct;60(5):1615-1629. doi: 10.1007/s10528-021-10169-1. Epub 
      2022 Jan 22.