PMID- 35065610
OWN - NLM
STAT- MEDLINE
DCOM- 20220316
LR  - 20220531
IS  - 1471-2172 (Electronic)
IS  - 1471-2172 (Linking)
VI  - 23
IP  - 1
DP  - 2022 Jan 22
TI  - STAT3 and SPI1, may lead to the immune system dysregulation and heterotopic 
      ossification in ankylosing spondylitis.
PG  - 3
LID - 10.1186/s12865-022-00476-6 [doi]
LID - 3
AB  - OBJECTIVE: This study was aimed to identify the biomarkers for diagnosis and 
      reveal the immune microenvironment changes in ankylosing spondylitis (AS). 
      METHODS: GSE73754 was downloaded for the co-expression network construction and 
      immune cell analyses. Flow cytometric analysis was performed to validate the 
      results of bioinformatics analysis. Gene set enrichment analysis (GSEA) was 
      performed to investigate the potential biological characteristic between 
      different phenotypes. Pearson correlation analysis between the hub genes and the 
      xCell score of immune cell types was performed. RESULTS: Signal transducer and 
      activator of transcription 3 (STAT3) and Spi-1 proto-oncogene (SPI1) was 
      identified as the hub genes in the datasets GSE73754. And the t-test showed that 
      the expression level of STAT3 and SPI1 in the GSE73754 was significantly higher 
      in AS and human leukocyte antigen (HLA)-B27(+) groups. Flow cytometric analysis 
      showed that natural killer T cells (NKT) cells were upregulated, while Th1 cells 
      were down-regulated in AS, which was consistent with the results obtained from 
      bioinformatics analysis. STAT3 and SPI1 was correlated with the NKT cells and Th1 
      cells. CONCLUSION: STAT3 and SPI1 may be a key cytokine receptor in disease 
      progression in AS.
CI  - (c) 2022. The Author(s).
FAU - Liang, Tuo
AU  - Liang T
AD  - Spine and Osteopathy Ward, The First Affiliated Hospital of Guangxi Medical 
      University, No.6 Shuangyong Road, Nanning, Guangxi, People's Republic of China.
FAU - Chen, Jiarui
AU  - Chen J
AD  - Spine and Osteopathy Ward, The First Affiliated Hospital of Guangxi Medical 
      University, No.6 Shuangyong Road, Nanning, Guangxi, People's Republic of China.
FAU - Xu, GuoYong
AU  - Xu G
AD  - Spine and Osteopathy Ward, The First Affiliated Hospital of Guangxi Medical 
      University, No.6 Shuangyong Road, Nanning, Guangxi, People's Republic of China.
FAU - Zhang, Zide
AU  - Zhang Z
AD  - Spine and Osteopathy Ward, The First Affiliated Hospital of Guangxi Medical 
      University, No.6 Shuangyong Road, Nanning, Guangxi, People's Republic of China.
FAU - Xue, Jiang
AU  - Xue J
AD  - Spine and Osteopathy Ward, The First Affiliated Hospital of Guangxi Medical 
      University, No.6 Shuangyong Road, Nanning, Guangxi, People's Republic of China.
FAU - Zeng, Haopeng
AU  - Zeng H
AD  - Spine and Osteopathy Ward, The First Affiliated Hospital of Guangxi Medical 
      University, No.6 Shuangyong Road, Nanning, Guangxi, People's Republic of China.
FAU - Jiang, Jie
AU  - Jiang J
AD  - Spine and Osteopathy Ward, The First Affiliated Hospital of Guangxi Medical 
      University, No.6 Shuangyong Road, Nanning, Guangxi, People's Republic of China.
FAU - Chen, Tianyou
AU  - Chen T
AD  - Spine and Osteopathy Ward, The First Affiliated Hospital of Guangxi Medical 
      University, No.6 Shuangyong Road, Nanning, Guangxi, People's Republic of China.
FAU - Qin, Zhaojie
AU  - Qin Z
AD  - Spine and Osteopathy Ward, The First Affiliated Hospital of Guangxi Medical 
      University, No.6 Shuangyong Road, Nanning, Guangxi, People's Republic of China.
FAU - Li, Hao
AU  - Li H
AD  - Spine and Osteopathy Ward, The First Affiliated Hospital of Guangxi Medical 
      University, No.6 Shuangyong Road, Nanning, Guangxi, People's Republic of China.
FAU - Ye, Zhen
AU  - Ye Z
AD  - Spine and Osteopathy Ward, The First Affiliated Hospital of Guangxi Medical 
      University, No.6 Shuangyong Road, Nanning, Guangxi, People's Republic of China.
FAU - Nie, Yunfeng
AU  - Nie Y
AD  - Guangxi Medical University, No.22 Shuangyong Road, Nanning, Guangxi, People's 
      Republic of China.
FAU - Zhan, Xinli
AU  - Zhan X
AD  - Spine and Osteopathy Ward, The First Affiliated Hospital of Guangxi Medical 
      University, No.6 Shuangyong Road, Nanning, Guangxi, People's Republic of China.
FAU - Liu, Chong
AU  - Liu C
AD  - Spine and Osteopathy Ward, The First Affiliated Hospital of Guangxi Medical 
      University, No.6 Shuangyong Road, Nanning, Guangxi, People's Republic of China. 
      lcgxykdx@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220122
PL  - England
TA  - BMC Immunol
JT  - BMC immunology
JID - 100966980
RN  - 0 (HLA-B27 Antigen)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (STAT3 protein, human)
RN  - 0 (Trans-Activators)
RN  - 0 (proto-oncogene protein Spi-1)
SB  - IM
MH  - HLA-B27 Antigen/analysis/metabolism
MH  - Humans
MH  - Immune System
MH  - *Ossification, Heterotopic
MH  - Proto-Oncogene Proteins
MH  - STAT3 Transcription Factor
MH  - *Spondylitis, Ankylosing
MH  - Trans-Activators
PMC - PMC8783415
OTO - NOTNLM
OT  - Ankylosing spondylitis
OT  - Biomarkers
OT  - NKT cells
OT  - SPI1
OT  - STAT3
OT  - Th1 cells
COIS- We declare that we do not have any competing interests.
EDAT- 2022/01/24 06:00
MHDA- 2022/03/17 06:00
PMCR- 2022/01/22
CRDT- 2022/01/23 20:26
PHST- 2021/10/03 00:00 [received]
PHST- 2022/01/12 00:00 [accepted]
PHST- 2022/01/23 20:26 [entrez]
PHST- 2022/01/24 06:00 [pubmed]
PHST- 2022/03/17 06:00 [medline]
PHST- 2022/01/22 00:00 [pmc-release]
AID - 10.1186/s12865-022-00476-6 [pii]
AID - 476 [pii]
AID - 10.1186/s12865-022-00476-6 [doi]
PST - epublish
SO  - BMC Immunol. 2022 Jan 22;23(1):3. doi: 10.1186/s12865-022-00476-6.