PMID- 35068144 OWN - NLM STAT- MEDLINE DCOM- 20220203 LR - 20220203 IS - 1520-5118 (Electronic) IS - 0021-8561 (Linking) VI - 70 IP - 4 DP - 2022 Feb 2 TI - Bioprocessing of Pea Protein can Enhance Fortified Fe But Reduce Zn In Vitro Bioaccessibility. PG - 1241-1251 LID - 10.1021/acs.jafc.1c05796 [doi] AB - The bioaccessibility of minerals during food digestion is essential in facilitating absorption and hence mineral bioavailability. Bioprocessing approaches have shown promising effects on Fe and Zn bioaccessibility in plant food matrices. In this study, lactic acid bacteria fermentation or enzymatic hydrolysis was performed on pea protein concentrates (PPCs) to investigate their effects on the bioaccessibility of fortified Fe and Zn salts. Simulated digestion studies revealed that enzymatic hydrolysis was more effective than fermentation. Phytase treatment significantly (P < 0.05) improved Fe(3+) bioaccessibility by 5- and 12-fold during fasted and fed digestion stages, respectively. Combined phytase and protease hydrolysis led to a 6- and 15-fold enhancement of Fe(3+) bioaccessibility during these stages. None of the bioprocessing approaches led to significant promotive effects on Zn(2+) bioaccessibility during fasted or fed digestion. Results of this study show the potential of enzymatic treatment of PPC to significantly promote Fe bioaccessibility. FAU - Zhang, Yianna Y AU - Zhang YY AUID- ORCID: 0000-0001-5765-8477 AD - School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3052, Australia. AD - CSIRO Agriculture & Food, 671 Sneydes Road, Werribee, Victoria 3030, Australia. FAU - Stockmann, Regine AU - Stockmann R AD - CSIRO Agriculture & Food, 671 Sneydes Road, Werribee, Victoria 3030, Australia. FAU - Ng, Ken AU - Ng K AD - School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3052, Australia. FAU - Ajlouni, Said AU - Ajlouni S AD - School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3052, Australia. LA - eng PT - Journal Article DEP - 20220124 PL - United States TA - J Agric Food Chem JT - Journal of agricultural and food chemistry JID - 0374755 RN - 0 (Minerals) RN - 0 (Pea Proteins) RN - EC 3.1.3.26 (6-Phytase) RN - J41CSQ7QDS (Zinc) SB - IM MH - *6-Phytase MH - Biological Availability MH - Digestion MH - Minerals MH - *Pea Proteins MH - Zinc OTO - NOTNLM OT - bioaccessibility OT - bioprocessing OT - in vitro digestion OT - iron OT - legumes OT - proteins/peptides OT - zinc EDAT- 2022/01/25 06:00 MHDA- 2022/02/04 06:00 CRDT- 2022/01/24 08:44 PHST- 2022/01/25 06:00 [pubmed] PHST- 2022/02/04 06:00 [medline] PHST- 2022/01/24 08:44 [entrez] AID - 10.1021/acs.jafc.1c05796 [doi] PST - ppublish SO - J Agric Food Chem. 2022 Feb 2;70(4):1241-1251. doi: 10.1021/acs.jafc.1c05796. Epub 2022 Jan 24.