PMID- 35068480
OWN - NLM
STAT- MEDLINE
DCOM- 20220405
LR  - 20230111
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 179
DP  - 2022 Jan 10
TI  - Single-Particle Interferometric Reflectance Imaging Characterization of 
      Individual Extracellular Vesicles and Population Dynamics.
LID - 10.3791/62988 [doi]
AB  - Extracellular vesicles (EVs) are nanometer-sized vesicles with a lipid bilayer 
      that are secreted by most cells. EVs carry a multitude of different biological 
      molecules, including protein, lipid, DNA, and RNA, and are postulated to 
      facilitate cell-to-cell communication in diverse tissues and organs. Recently, 
      EVs have attracted significant attention as biomarkers for diagnostics and 
      therapeutic agents for various diseases. Many methods have been developed for EV 
      characterization. However, current methods for EV analysis all have different 
      limitations. Thus, developing efficient and effective methods for EV isolation 
      and characterization remains one of the crucial steps for this cutting-edge 
      research field as it matures. Here, we provide a detailed protocol outlining a 
      single-particle interferometric reflectance imaging sensor (SP-IRIS), as a method 
      that is capable of detecting and characterizing EVs from unpurified biological 
      sources and purified EVs by other methodologies. This advanced technique can be 
      used for multi-level and comprehensive measurements for the analysis of EV size, 
      EV count, EV phenotype, and biomarker colocalization.
FAU - Deng, Fengyan
AU  - Deng F
AD  - Department of Molecular and Integrative Physiology, University of Kansas Medical 
      Center.
FAU - Ratri, Anamika
AU  - Ratri A
AD  - Department of Molecular and Integrative Physiology, University of Kansas Medical 
      Center.
FAU - Deighan, Clayton
AU  - Deighan C
AD  - NanoView Biosciences.
FAU - Daaboul, George
AU  - Daaboul G
AD  - NanoView Biosciences.
FAU - Geiger, Paige C
AU  - Geiger PC
AD  - Department of Molecular and Integrative Physiology, University of Kansas Medical 
      Center.
FAU - Christenson, Lane K
AU  - Christenson LK
AD  - Department of Molecular and Integrative Physiology, University of Kansas Medical 
      Center; lchristenson@kumc.edu.
LA  - eng
GR  - R21 AG066488/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20220110
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 0 (Biomarkers)
RN  - 0 (Proteins)
SB  - IM
MH  - Biomarkers/metabolism
MH  - *Extracellular Vesicles/metabolism
MH  - Interferometry
MH  - Population Dynamics
MH  - Proteins/metabolism
PMC - PMC8968924
MID - NIHMS1784125
EDAT- 2022/01/25 06:00
MHDA- 2022/04/05 06:00
PMCR- 2023/01/10
CRDT- 2022/01/24 08:46
PHST- 2022/01/24 08:46 [entrez]
PHST- 2022/01/25 06:00 [pubmed]
PHST- 2022/04/05 06:00 [medline]
PHST- 2023/01/10 00:00 [pmc-release]
AID - 10.3791/62988 [doi]
PST - epublish
SO  - J Vis Exp. 2022 Jan 10;(179):10.3791/62988. doi: 10.3791/62988.