PMID- 35069539
OWN - NLM
STAT- MEDLINE
DCOM- 20220221
LR  - 20220221
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 12
DP  - 2021
TI  - Granulocyte Macrophage-Colony Stimulating Factor Produces a Splenic Subset of 
      Monocyte-Derived Dendritic Cells That Efficiently Polarize T Helper Type 2 Cells 
      in Response to Blood-Borne Antigen.
PG  - 767037
LID - 10.3389/fimmu.2021.767037 [doi]
LID - 767037
AB  - Dendritic cells (DCs) are key antigen-presenting cells that prime naive T cells 
      and initiate adaptive immunity. Although the genetic deficiency and transgenic 
      overexpression of granulocyte macrophage-colony stimulating factor (GM-CSF) 
      signaling were reported to influence the homeostasis of DCs, the in vivo 
      development of DC subsets following injection of GM-CSF has not been analyzed in 
      detail. Among the treatment of mice with different hematopoietic cytokines, only 
      GM-CSF generates a distinct subset of XCR1(-)33D1(-) DCs which make up the 
      majority of DCs in the spleen after three daily injections. These GM-CSF-induced 
      DCs (GMiDCs) are distinguished from classical DCs (cDCs) in the spleen by their 
      expression of CD115 and CD301b and by their superior ability to present 
      blood-borne antigen and thus to stimulate CD4(+) T cells. Unlike cDCs in the 
      spleen, GMiDCs are exceptionally effective to polarize and expand T helper type 2 
      (Th2) cells and able to induce allergic sensitization in response to blood-borne 
      antigen. Single-cell RNA sequencing analysis and adoptive cell transfer assay 
      reveal the sequential differentiation of classical monocytes into pre-GMiDCs and 
      GMiDCs. Interestingly, mixed bone marrow chimeric mice of Csf2rb(+/+) and 
      Csf2rb(-/-) demonstrate that the generation of GMiDCs necessitates the cis 
      expression of GM-CSF receptor. Besides the spleen, GMiDCs are generated in the 
      CCR7-independent resident DCs of the LNs and in some peripheral tissues with 
      GM-CSF treatment. Also, small but significant numbers of GMiDCs are generated in 
      the spleen and other tissues during chronic allergic inflammation. Collectively, 
      our present study identifies a splenic subset of CD115(hi)CD301b(+) GMiDCs that 
      possess a strong capacity to promote Th2 polarization and allergic sensitization 
      against blood-borne antigen.
CI  - Copyright (c) 2022 Ryu, Shin, Eum, Park, Choi, Na, In, Kim, Park, Hwang, Sohn, Kim, 
      Seo, Lee, Lee, Chu and Park.
FAU - Ryu, Seul Hye
AU  - Ryu SH
AD  - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei 
      University College of Medicine, Seoul, South Korea.
AD  - Brain Korea 21 FOUR Project for Medical Science, Yonsei University College of 
      Medicine, Seoul, South Korea.
AD  - Immune and Vascular Cell Network Research Center, National Creative Initiatives, 
      Department of Life Sciences, Ewha Womans University, Seoul, South Korea.
FAU - Shin, Hyun Soo
AU  - Shin HS
AD  - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei 
      University College of Medicine, Seoul, South Korea.
AD  - Brain Korea 21 FOUR Project for Medical Science, Yonsei University College of 
      Medicine, Seoul, South Korea.
FAU - Eum, Hye Hyeon
AU  - Eum HH
AD  - Department of Biomedicine and Health Sciences, Graduate School, The Catholic 
      University of Korea, Seoul, South Korea.
AD  - Department of Microbiology, College of Medicine, The Catholic University of 
      Korea, Seoul, South Korea.
FAU - Park, Ji Soo
AU  - Park JS
AD  - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei 
      University College of Medicine, Seoul, South Korea.
AD  - Brain Korea 21 FOUR Project for Medical Science, Yonsei University College of 
      Medicine, Seoul, South Korea.
FAU - Choi, Wanho
AU  - Choi W
AD  - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei 
      University College of Medicine, Seoul, South Korea.
AD  - Brain Korea 21 FOUR Project for Medical Science, Yonsei University College of 
      Medicine, Seoul, South Korea.
FAU - Na, Hye Young
AU  - Na HY
AD  - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei 
      University College of Medicine, Seoul, South Korea.
AD  - Department of Neurology, Severance Hospital, Yonsei University College of 
      Medicine, Seoul, South Korea.
FAU - In, Hyunju
AU  - In H
AD  - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei 
      University College of Medicine, Seoul, South Korea.
AD  - Brain Korea 21 FOUR Project for Medical Science, Yonsei University College of 
      Medicine, Seoul, South Korea.
FAU - Kim, Tae-Gyun
AU  - Kim TG
AD  - Department of Dermatology, Severance Hospital, Cutaneous Biology Research 
      Institute, Yonsei University College of Medicine, Seoul, South Korea.
FAU - Park, Sejung
AU  - Park S
AD  - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei 
      University College of Medicine, Seoul, South Korea.
AD  - Brain Korea 21 FOUR Project for Medical Science, Yonsei University College of 
      Medicine, Seoul, South Korea.
FAU - Hwang, Soomin
AU  - Hwang S
AD  - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei 
      University College of Medicine, Seoul, South Korea.
AD  - Brain Korea 21 FOUR Project for Medical Science, Yonsei University College of 
      Medicine, Seoul, South Korea.
FAU - Sohn, Moah
AU  - Sohn M
AD  - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei 
      University College of Medicine, Seoul, South Korea.
AD  - Brain Korea 21 FOUR Project for Medical Science, Yonsei University College of 
      Medicine, Seoul, South Korea.
FAU - Kim, Eun-Do
AU  - Kim ED
AD  - Department of Ophthalmology, Severance Hospital, Institute of Vision Research, 
      Yonsei University College of Medicine, Seoul, South Korea.
FAU - Seo, Kyoung Yul
AU  - Seo KY
AD  - Department of Ophthalmology, Severance Hospital, Institute of Vision Research, 
      Yonsei University College of Medicine, Seoul, South Korea.
FAU - Lee, Hae-Ock
AU  - Lee HO
AD  - Department of Biomedicine and Health Sciences, Graduate School, The Catholic 
      University of Korea, Seoul, South Korea.
AD  - Department of Microbiology, College of Medicine, The Catholic University of 
      Korea, Seoul, South Korea.
FAU - Lee, Min-Geol
AU  - Lee MG
AD  - Department of Dermatology, Severance Hospital, Cutaneous Biology Research 
      Institute, Yonsei University College of Medicine, Seoul, South Korea.
FAU - Chu, Min Kyung
AU  - Chu MK
AD  - Department of Neurology, Severance Hospital, Yonsei University College of 
      Medicine, Seoul, South Korea.
FAU - Park, Chae Gyu
AU  - Park CG
AD  - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei 
      University College of Medicine, Seoul, South Korea.
AD  - Therapeutic Antibody Research Center, Genuv Inc., Seoul, South Korea.
AD  - Institute for Immunology and Immunological Diseases, Yonsei University College of 
      Medicine, Seoul, South Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220103
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Antigens)
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/immunology
MH  - Antigens/*immunology
MH  - Cell Differentiation/immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*immunology
MH  - Granulocytes/*immunology
MH  - Macrophages/*immunology
MH  - Membrane Proteins/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Monocytes/*immunology
MH  - Receptors, Granulocyte-Macrophage Colony-Stimulating Factor/immunology
MH  - Spleen/*immunology
MH  - Th2 Cells/*immunology
PMC - PMC8778578
OTO - NOTNLM
OT  - GM-CSF
OT  - GM-CSF receptor
OT  - T cell - DC interactions
OT  - Th2 cell
OT  - allergic sensitization
OT  - dendritic cell
OT  - monocyte-derived dendritic cell
OT  - spleen
COIS- Author CP is employed by Genuv Inc. The remaining authors declare that the 
      research was conducted in the absence of any commercial or financial 
      relationships that could be construed as a potential conflict of interest.
EDAT- 2022/01/25 06:00
MHDA- 2022/02/22 06:00
PMCR- 2021/01/01
CRDT- 2022/01/24 08:49
PHST- 2021/08/30 00:00 [received]
PHST- 2021/12/06 00:00 [accepted]
PHST- 2022/01/24 08:49 [entrez]
PHST- 2022/01/25 06:00 [pubmed]
PHST- 2022/02/22 06:00 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2021.767037 [doi]
PST - epublish
SO  - Front Immunol. 2022 Jan 3;12:767037. doi: 10.3389/fimmu.2021.767037. eCollection 
      2021.