PMID- 35072186
OWN - NLM
STAT- MEDLINE
DCOM- 20220225
LR  - 20220225
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 13
IP  - 3
DP  - 2022 Feb 7
TI  - Sesquiterpene glycoside isolated from loquat leaf targets gut microbiota to 
      prevent type 2 diabetes mellitus in db/db mice.
PG  - 1519-1534
LID - 10.1039/d1fo03646g [doi]
AB  - According to ancient records, loquat leaf has been used as both a food and 
      medicine in China for thousands of years. Sesquiterpene glycosides from loquat 
      leaf have achieved remarkable effects on hyperglycemia. However, their specific 
      activities and underlying mechanisms on type 2 diabetes mellitus (T2DM) are not 
      fully understood. In the present study, we found that SG1, a unique sesquiterpene 
      glycoside isolated from loquat leaf, had the capability to prevent insulin 
      resistance and inflammation. In db/db mice, SG1 administration (25 and 50 mg 
      kg(-1) day(-1)) inhibited hyperglycemia and the release of inflammatory 
      cytokines. To further explore the possible role of gut microbiota in SG1 for 
      treating T2DM, we applied 16S rRNA pyrosequencing based on the V3-V4 region to 
      analyze the fecal samples of different groups. Alpha diversity analysis showed 
      that SG1 administration could obviously increase diversity and richness in db/db 
      mice. At the phylum level, due to SG1 treatment, the relative abundance of 
      Firmicutes and Actinobacteria was lowered while that of Bacteroidetes was raised. 
      Additionally, 7 key genera in the db/db mice with SG1 supplementation were 
      enriched: Lactobacillus, Lachnospiraceae_NK4A136_group, and Ruminococcus, 
      Bacteroides, Prevotellaceae_UCG-001, Alistipes, and Roseburia. These findings 
      proved that SG1 could prevent T2DM by relieving insulin resistance and 
      inflammation and by remodeling the gut microbiota in db/db mice.
FAU - Wu, Ruoyun
AU  - Wu R
AUID- ORCID: 0000-0003-2431-2423
AD  - Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 
      210014, China. jiantunyu1986@163.com.
FAU - Zhou, Lina
AU  - Zhou L
AD  - Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 
      210014, China. jiantunyu1986@163.com.
FAU - Chen, Yan
AU  - Chen Y
AD  - Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 
      210014, China. jiantunyu1986@163.com.
FAU - Ding, Xiaoqin
AU  - Ding X
AUID- ORCID: 0000-0002-9365-6396
AD  - Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 
      210014, China. jiantunyu1986@163.com.
FAU - Liu, Yan
AU  - Liu Y
AD  - Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 
      210014, China. jiantunyu1986@163.com.
FAU - Tong, Bei
AU  - Tong B
AD  - Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 
      210014, China. jiantunyu1986@163.com.
FAU - Lv, Han
AU  - Lv H
AUID- ORCID: 0000-0003-4452-4351
AD  - Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 
      210014, China. jiantunyu1986@163.com.
FAU - Meng, Xiuhua
AU  - Meng X
AD  - Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 
      210014, China. jiantunyu1986@163.com.
FAU - Li, Jing
AU  - Li J
AD  - Department of Food Science and Technology, College of Light Industry and Food 
      Engineering, Nanjing Forestry University, Nanjing 210037, China. 
      chenjian80@aliyun.com.
FAU - Jian, Tunyu
AU  - Jian T
AD  - Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 
      210014, China. jiantunyu1986@163.com.
FAU - Chen, Jian
AU  - Chen J
AUID- ORCID: 0000-0003-0686-833X
AD  - Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 
      210014, China. jiantunyu1986@163.com.
AD  - Department of Food Science and Technology, College of Light Industry and Food 
      Engineering, Nanjing Forestry University, Nanjing 210037, China. 
      chenjian80@aliyun.com.
LA  - eng
PT  - Journal Article
DEP - 20220207
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
RN  - 0 (Blood Glucose)
RN  - 0 (Glycosides)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Sesquiterpenes)
SB  - IM
MH  - Animals
MH  - Blood Glucose
MH  - Diabetes Mellitus, Type 2/*prevention & control
MH  - *Eriobotrya
MH  - Functional Food
MH  - Gastrointestinal Microbiome/drug effects
MH  - Glycosides/*pharmacology
MH  - Hypoglycemic Agents/*pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred Strains
MH  - Plant Leaves
MH  - Sesquiterpenes/*pharmacology
EDAT- 2022/01/25 06:00
MHDA- 2022/02/26 06:00
CRDT- 2022/01/24 08:58
PHST- 2022/01/25 06:00 [pubmed]
PHST- 2022/02/26 06:00 [medline]
PHST- 2022/01/24 08:58 [entrez]
AID - 10.1039/d1fo03646g [doi]
PST - epublish
SO  - Food Funct. 2022 Feb 7;13(3):1519-1534. doi: 10.1039/d1fo03646g.