PMID- 35072275
OWN - NLM
STAT- MEDLINE
DCOM- 20221014
LR  - 20221215
IS  - 1554-527X (Electronic)
IS  - 0736-0266 (Linking)
VI  - 40
IP  - 11
DP  - 2022 Nov
TI  - Similarity and difference between aging and puncture-induced intervertebral disc 
      degeneration.
PG  - 2565-2575
LID - 10.1002/jor.25281 [doi]
AB  - The purpose of our study was to investigate the changes in micromorphology and 
      mechanical properties of intervertebral discs degeneration induced by aging and 
      puncture. Normal group (NG), 2 weeks post-puncture degeneration group (PDG) and 
      aging degeneration group (ADG) each included 10 rats. Plain film, magnetic 
      resonance imaging, and histological testing were utilized to assess 
      intervertebral disc degeneration. Atomic force microscope was utilized to analyze 
      the microstructure and elastic modulus of the intervertebral disc, while 
      immunohistochemistry was employed to assess alterations in the cell matrix using 
      collagen I, collagen II, matrix metalloproteinase-3 (MMP-3), and tumour necrosis 
      factor-alpha (TNF-alpha). The results showed that the disc height ratio between PDG and 
      ADG decreased. In the PDG and ADG group, histological scores both increased, the 
      gray value of the T2 signal decreased, the proportion of MMP-3 and TNF-positive 
      cells in intervertebral disc tissues was higher (p < 0.05) and the IOD values of 
      COL-2 lower in intervertebral disc tissues (p < 0.05). The elastic modulus of PDG 
      and ADG annulus fibers (AF) increased compared to the NG (p < 0.05); when 
      compared to PDG, the elastic modulus of ADG AF decreased (p < 0.05). The elastic 
      modulus of PDG and ADG collagen increased in the nucleus pulposus (NP, p < 0.05); 
      ADG had a greater AF diameter than NG and PDG (p < 0.05). The results indicated 
      that ADG fiber diameter thickens, and chronic inflammation indicators rise; PDG 
      suffers from severe extracellular matrix loss. The degeneration of the ADG and 
      PDG intervertebral discs is different. The results provide foundation for 
      clinical research.
CI  - (c) 2022 Orthopaedic Research Society. Published by Wiley Periodicals LLC.
FAU - He, Shuangjun
AU  - He S
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu, China.
AD  - Department of Orthopedic Surgery, Affiliated Danyang Hospital of Nantong 
      University, The People's Hospital of Danyang, Danyang, Jiangsu, China.
FAU - Zhang, Yijian
AU  - Zhang Y
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu, China.
FAU - Zhou, Zhangzhe
AU  - Zhou Z
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu, China.
FAU - Shao, Xiaofeng
AU  - Shao X
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu, China.
FAU - Chen, Kangwu
AU  - Chen K
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu, China.
FAU - Dai, Shouqian
AU  - Dai S
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu, China.
FAU - Liang, Ting
AU  - Liang T
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu, China.
AD  - Department of Orthopaedics, Orthopaedic Institute, The First Affiliated Hospital, 
      Soochow University, Suzhou, Jiangsu, China.
FAU - Qian, Zhonglai
AU  - Qian Z
AUID- ORCID: 0000-0002-0737-9696
AD  - Department of Orthopaedics, The First Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu, China.
FAU - Luo, Zongping
AU  - Luo Z
AD  - Department of Orthopaedics, Orthopaedic Institute, The First Affiliated Hospital, 
      Soochow University, Suzhou, Jiangsu, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220204
PL  - United States
TA  - J Orthop Res
JT  - Journal of orthopaedic research : official publication of the Orthopaedic 
      Research Society
JID - 8404726
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Aging
MH  - Animals
MH  - Collagen
MH  - Disease Models, Animal
MH  - *Intervertebral Disc/pathology
MH  - *Intervertebral Disc Degeneration/diagnostic imaging/etiology/pathology
MH  - Matrix Metalloproteinase 3
MH  - Punctures
MH  - Rats
MH  - Tumor Necrosis Factor-alpha
OTO - NOTNLM
OT  - atomic force microscopy
OT  - biomechanics
OT  - collagen fiber
OT  - elastic modulus
OT  - intervertebral disc
EDAT- 2022/01/25 06:00
MHDA- 2022/10/15 06:00
CRDT- 2022/01/24 08:59
PHST- 2022/01/10 00:00 [revised]
PHST- 2021/07/22 00:00 [received]
PHST- 2022/01/16 00:00 [accepted]
PHST- 2022/01/25 06:00 [pubmed]
PHST- 2022/10/15 06:00 [medline]
PHST- 2022/01/24 08:59 [entrez]
AID - 10.1002/jor.25281 [doi]
PST - ppublish
SO  - J Orthop Res. 2022 Nov;40(11):2565-2575. doi: 10.1002/jor.25281. Epub 2022 Feb 4.