PMID- 35075546
OWN - NLM
STAT- MEDLINE
DCOM- 20220127
LR  - 20220218
IS  - 1573-0972 (Electronic)
IS  - 0959-3993 (Linking)
VI  - 38
IP  - 3
DP  - 2022 Jan 25
TI  - Bioreduction and bioremoval of hexavalent chromium by genetically engineered 
      strains (Escherichia coli MT2A and Escherichia coli MT3).
PG  - 45
LID - 10.1007/s11274-022-03235-2 [doi]
AB  - The number of studies on the removal of hazardous metals from water using genetic 
      engineering technologies is growing. A high rate of metal ion removal from the 
      environment is ensured, particularly through the expression of cysteine and 
      thiol-rich proteins such as metallothioneins in bacterial cells. In this study, 
      we used recombinant strains created by cloning the human metallothioneins MT2A 
      and MT3 into Escherichia coli Jm109 to assess the removal and reduction of 
      hexavalent chromium (Cr(VI)) from aqueous solutions. MT2A was the most effective 
      strain in both Cr(VI) removal (89% in 25 mg/L Cr(VI)) and Cr(VI) reduction (76% 
      in 25 mg/L Cr(VI)). The amount of Cr adsorbed per dry cell by the MT2A strain was 
      22 mg/g. The biosorption of total Cr was consistent with the Langmuir isotherm 
      model. Scanning electron microscope (SEM) images revealed that the morphological 
      structures of Cr(VI)-treated cells were significantly damaged when compared to 
      control cells. Scanning transmission electron microscope (STEM) images showed 
      black spots in the cytoplasm of cells treated with Cr(VI). Shifts in the Fourier 
      transform infrared spectroscopy analysis (FTIR) spectra of the cells treated with 
      Cr(VI) showed that the groups interacting with Cr were hydroxyl, amine, amide I, 
      amide II, phosphoryl and carbonyl. When all of the experimental data was 
      combined, it was determined that both MT2A and MT3 were effective in removing 
      Cr(VI) from aqueous solutions, but MT2A was more effective, indicating that MT2A 
      may be employed as a biotechnological tool.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Akkurt, Seyma
AU  - Akkurt S
AUID- ORCID: 0000-0002-0135-1975
AD  - Department of Environmental Engineering, Faculty of Engineering, Adiyaman 
      University, Altinsehir Neighborhood, Ataturk Boulevard, No. 1, Central Campus, 
      02040, Central, Adiyaman, Turkey.
FAU - Oguz, Merve
AU  - Oguz M
AUID- ORCID: 0000-0002-8388-1477
AD  - Department of Environmental Engineering, Faculty of Engineering, Erciyes 
      University, Kayseri, Turkey.
FAU - Alkan Uckun, Aysel
AU  - Alkan Uckun A
AUID- ORCID: 0000-0002-8957-7476
AD  - Department of Environmental Engineering, Faculty of Engineering, Adiyaman 
      University, Altinsehir Neighborhood, Ataturk Boulevard, No. 1, Central Campus, 
      02040, Central, Adiyaman, Turkey. auckun@adiyaman.edu.tr.
LA  - eng
GR  - FDK-2019-9543/erciyes universitesi/
PT  - Journal Article
DEP - 20220125
PL  - Germany
TA  - World J Microbiol Biotechnol
JT  - World journal of microbiology & biotechnology
JID - 9012472
RN  - 0 (Metals)
RN  - 0 (Water Pollutants, Chemical)
RN  - 0R0008Q3JB (Chromium)
RN  - 18540-29-9 (chromium hexavalent ion)
RN  - 9038-94-2 (Metallothionein)
SB  - IM
MH  - Adsorption
MH  - *Biodegradation, Environmental
MH  - Biotechnology/methods
MH  - Chromium/*metabolism
MH  - Cloning, Molecular
MH  - Escherichia coli/genetics/*metabolism
MH  - Genetic Engineering/methods
MH  - Humans
MH  - Metallothionein/genetics/*metabolism
MH  - Metals/*metabolism
MH  - Water Pollutants, Chemical/metabolism
MH  - Water Purification/methods
OTO - NOTNLM
OT  - Biological treatment
OT  - Bioremediation
OT  - Chromium removal
OT  - Cr(VI) bioreduction
OT  - Genetical engineered bacteria
OT  - Metalloproteins
EDAT- 2022/01/26 06:00
MHDA- 2022/01/28 06:00
CRDT- 2022/01/25 05:50
PHST- 2021/07/12 00:00 [received]
PHST- 2022/01/13 00:00 [accepted]
PHST- 2022/01/25 05:50 [entrez]
PHST- 2022/01/26 06:00 [pubmed]
PHST- 2022/01/28 06:00 [medline]
AID - 10.1007/s11274-022-03235-2 [pii]
AID - 10.1007/s11274-022-03235-2 [doi]
PST - epublish
SO  - World J Microbiol Biotechnol. 2022 Jan 25;38(3):45. doi: 
      10.1007/s11274-022-03235-2.