PMID- 35076076
OWN - NLM
STAT- MEDLINE
DCOM- 20220203
LR  - 20220430
IS  - 1460-2709 (Electronic)
IS  - 1369-3786 (Linking)
VI  - 60
IP  - 2
DP  - 2022 Feb 2
TI  - Biochemical characterization and analysis of gene expression of an alpha-mannosidase 
      secreted by Paracoccidioides brasiliensis.
LID - myac002 [pii]
LID - 10.1093/mmy/myac002 [doi]
AB  - Paracoccidioidomycosis (PCM) is a systemic mycosis caused by fungi of the 
      Paracoccidioides genus, being endemic in Latin America and with the highest 
      number of cases in Brazil. Paracoccidioides spp. release a wide range of 
      molecules, such as enzymes, which may be important for PCM establishment. Here, 
      we identified the 85- and 90-kDa proteins from the supernatants of P. 
      brasiliensis cultures as being an alpha-mannosidase. Because the expected mass of 
      this alpha-mannosidase is 124.2-kDa, we suggest that the proteins were cleavage 
      products. Indeed, we found an alpha-mannosidase activity in the culture supernatants 
      among the excreted/secreted antigens (ESAg). Moreover, we determined that the 
      enzyme activity was optimal in buffer at pH 5.6, at the temperature of 45 masculineC, and 
      with a concentration of 3 mM of the substrate p-NP-alpha-D-Man. Remarkably, we showed 
      that the gene expression of this alpha-mannosidase was higher in yeasts than hyphae 
      in three P. brasiliensis isolates with different virulence degrees that were 
      grown in Ham's F12 synthetic medium for 15 days. But in complex media YPD and 
      Fava Netto, the significantly higher gene expression in yeasts than in hyphae was 
      seen only for the virulent isolate Pb18, but not for intermediate virulence Pb339 
      and low virulence Pb265 isolates. These results about the high expression of the 
      alpha-mannosidase gene in the pathogenic yeast form of P. brasiliensis open 
      perspectives for studying this alpha-mannosidase concerning the virulence of P. 
      brasiliensis isolates. LAY SUMMARY: Paracoccidioides brasiliensis causes deep 
      mycosis, paracoccidioidomycosis. We determined for the first time the biochemical 
      properties of an alpha-mannosidase released by this fungus. We suggest that the 
      enzyme gene expression in the fungus is associated with fungal morphology, 
      stress, and virulence.
CI  - (c) The Author(s) 2022. Published by Oxford University Press on behalf of The 
      International Society for Human and Animal Mycology.
FAU - Correa, Priscila C
AU  - Correa PC
AD  - Graduate Program in Basic and Applied Immunology, Ribeirao Preto Medical School, 
      University of Sao Paulo, 14049-900, Ribeirao Preto, SP, Brazil.
FAU - Fernandes, Fabricio F
AU  - Fernandes FF
AD  - Paulista University, 14024-270, Ribeirao Preto, SP, Brazil.
FAU - Costa, Marcelo V
AU  - Costa MV
AD  - Department of Biology, Faculty of Philosophy, Sciences and Letters at Ribeirao 
      Preto, University of Sao Paulo, 14040-901, Ribeirao Preto, SP, Brazil.
FAU - Landgraf, Taise N
AU  - Landgraf TN
AD  - Anhanguera Educational, Leme, 13614-370, SP, Brazil.
FAU - Panunto-Castelo, Ademilson
AU  - Panunto-Castelo A
AUID- ORCID: 0000-0003-0148-0133
AD  - Department of Biology, Faculty of Philosophy, Sciences and Letters at Ribeirao 
      Preto, University of Sao Paulo, 14040-901, Ribeirao Preto, SP, Brazil.
LA  - eng
GR  - 2017/01390-8/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/
GR  - Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/
PT  - Journal Article
PL  - England
TA  - Med Mycol
JT  - Medical mycology
JID - 9815835
RN  - EC 3.2.1.24 (alpha-Mannosidase)
SB  - IM
MH  - Animals
MH  - Gene Expression
MH  - *Paracoccidioides/genetics
MH  - *Paracoccidioidomycosis/veterinary
MH  - Virulence
MH  - alpha-Mannosidase/genetics
OTO - NOTNLM
OT  - Paracoccidioides. Extracellular antigens. Glycosyl hydrolase. alpha-Mannosidase
OT  - Fungal infection
EDAT- 2022/01/26 06:00
MHDA- 2022/02/04 06:00
CRDT- 2022/01/25 08:55
PHST- 2021/08/12 00:00 [received]
PHST- 2021/12/14 00:00 [revised]
PHST- 2022/01/26 06:00 [pubmed]
PHST- 2022/02/04 06:00 [medline]
PHST- 2022/01/25 08:55 [entrez]
AID - 6514532 [pii]
AID - 10.1093/mmy/myac002 [doi]
PST - ppublish
SO  - Med Mycol. 2022 Feb 2;60(2):myac002. doi: 10.1093/mmy/myac002.