PMID- 35077876
OWN - NLM
STAT- MEDLINE
DCOM- 20220308
LR  - 20220308
IS  - 1873-1597 (Electronic)
IS  - 1572-1000 (Linking)
VI  - 37
DP  - 2022 Mar
TI  - In-vitro co-delivery of decarbazine and photosense using poly lactic-co-glycolic 
      acid nanocarrier for combinational therapy.
PG  - 102737
LID - S1572-1000(22)00026-6 [pii]
LID - 10.1016/j.pdpdt.2022.102737 [doi]
AB  - PLGA (Poly lactic-co-glycolic acid) nanoparticles are a new trend for drug 
      delivery due to their good biodegradability properties. In this study, we have 
      synthesized PLGA nanoparticles by solvent evaporation method and loaded 
      decarbazine (DTIC, 5-3,3-(dimethyl-ltriazeno)imidazole-4-carboxamide) and 
      photosense (AlPc4) drug alone as well as combined with two different 
      concentrations i-e 25 nM and 250 nM. No cytotoxicity (viability  approximately  100%) was 
      observed for different treatment arms either alone or in co-delivery of 
      nano-formulation for Rhabdomyosarcoma (RD) cell culture which showed the 
      biocompatibility of carrier. On comparison, the Photodynamic therapy (PDT) alone 
      showed more significant cell death then the combinational therapy 
      (PDT + chemotherapy) at 2 joule /cm(2) and 5 joule /cm(2). Lower doses 
      co-delivery showed light dose dependent toxicity to culture i.e., 0% death @ 
      2 joule /cm(2),  approximately  40% death @ 5 joule /cm(2). Gene expressions of four apoptosis 
      related genes (CASP3, CASP9, PARP1 and P53) were quantified by RT-PCR which shows 
      down regulation for all the treatment arms indicating the absence of apoptosis 
      for the cell death during PDT and combinational therapy. It was concluded that 
      apoptosis related genes were down-regulated and morphological changes i.e., 
      swelling and disruption suggest that the mode of cell death was necrosis.
CI  - Copyright (c) 2022. Published by Elsevier B.V.
FAU - Arshad, Maryam
AU  - Arshad M
AD  - Cancer Genetics and Epigenetics Lab, Department of Biosciences, COMSATS 
      University, Islamabad, Pakistan.
FAU - Mahjabeen, Ishrat
AU  - Mahjabeen I
AD  - Cancer Genetics and Epigenetics Lab, Department of Biosciences, COMSATS 
      University, Islamabad, Pakistan.
FAU - Raza, Abida
AU  - Raza A
AD  - National Institute for Lasers & Optronics, Nilore, Islamabad, Pakistan.
FAU - Javaid, Sumbal
AU  - Javaid S
AD  - Department of Animal Sciences, Quaid-e-Azam University, Islamabad, Pakistan.
FAU - Fazal Ul Haq, Maria
AU  - Fazal Ul Haq M
AD  - Cancer Genetics and Epigenetics Lab, Department of Biosciences, COMSATS 
      University, Islamabad, Pakistan.
FAU - Alam, Masroor
AU  - Alam M
AD  - Department of Virology and Immunology, National Institute of Health (NIH), 
      Islamabad, Pakistan.
FAU - Khurshid, Ahmat
AU  - Khurshid A
AD  - Nanophotomedicine Laboratory, Pakistan Institute of Engineering and Applied 
      Sciences (PIEAS), Islamabad, Pakistan. Electronic address: ahmat@pieas.edu.pk.
LA  - eng
PT  - Journal Article
DEP - 20220122
PL  - Netherlands
TA  - Photodiagnosis Photodyn Ther
JT  - Photodiagnosis and photodynamic therapy
JID - 101226123
RN  - 0 (Drug Carriers)
RN  - 0 (Glycolates)
RN  - 0 (Glycols)
RN  - 0WT12SX38S (glycolic acid)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 7GR28W0FJI (Dacarbazine)
SB  - IM
MH  - Dacarbazine
MH  - Drug Carriers
MH  - Glycolates
MH  - Glycols
MH  - Lactic Acid
MH  - *Nanoparticles
MH  - Particle Size
MH  - *Photochemotherapy/methods
MH  - Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
OTO - NOTNLM
OT  - Combinational index (CI)
OT  - Combinational therapy
OT  - Decarbazine
OT  - PLGA nanoparticles
OT  - Photodynamic therapy
OT  - Photosense
OT  - RT-PCR
OT  - Rhabdomyosarcoma
OT  - Tumor suppressor genes
EDAT- 2022/01/26 06:00
MHDA- 2022/03/09 06:00
CRDT- 2022/01/25 20:12
PHST- 2021/08/27 00:00 [received]
PHST- 2021/12/24 00:00 [revised]
PHST- 2022/01/20 00:00 [accepted]
PHST- 2022/01/26 06:00 [pubmed]
PHST- 2022/03/09 06:00 [medline]
PHST- 2022/01/25 20:12 [entrez]
AID - S1572-1000(22)00026-6 [pii]
AID - 10.1016/j.pdpdt.2022.102737 [doi]
PST - ppublish
SO  - Photodiagnosis Photodyn Ther. 2022 Mar;37:102737. doi: 
      10.1016/j.pdpdt.2022.102737. Epub 2022 Jan 22.