PMID- 35077907
OWN - NLM
STAT- MEDLINE
DCOM- 20220223
LR  - 20220223
IS  - 1872-7077 (Electronic)
IS  - 1382-6689 (Linking)
VI  - 90
DP  - 2022 Feb
TI  - Enterobacter cloacae aggravates metabolic disease by inducing inflammation and 
      lipid accumulation.
PG  - 103819
LID - S1382-6689(22)00012-6 [pii]
LID - 10.1016/j.etap.2022.103819 [doi]
AB  - It is well known that gut microbiota imbalance can promote the development of 
      metabolic disease. Enterobacter cloacae (E. cloacae) is a kind of opportunistic 
      pathogen in the intestine. Therefore, we hypothesized that E. cloacae accelerated 
      the development of metabolic disease. To answer this question, we used E. cloacae 
      to induce disease in guinea pigs. We used H&E staining to detect the pathological 
      changes of liver and aorta and used Oil Red O staining to evaluate the lipid 
      accumulation in the liver. And that we used a kit to detect AST content and used 
      Western blot to detect protein levels in the liver. We found that E. cloacae 
      could induce liver pathological changes and lipid accumulation as well as aortic 
      wall pathological changes in guinea pigs. And E. cloacae increased the liver 
      index to 5.94% and the serum AST level to 41.93 U/L. Importantly, E. cloacae 
      activated liver high mobility group protein (HMGB1)/toll-like receptor 4 
      (TLR4)/myeloiddifferentiationfactor88 (MYD88)/nuclear factor-kappa B (NF-kappaB) 
      signal and sterol regulatory element-binding protein 1c (SREBP-1c) and inhibited 
      AMP-activated protein kinase (AMPK). We conclude that E. cloacae promote 
      nonalcoholic fatty liver disease (NAFLD) by inducing inflammation and lipid 
      accumulation, and E. cloacae also promote atherosclerosis. These findings are 
      important for study on the pathogenesis and drug screening of NAFLD and 
      atherosclerosis.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Jin, Meiyu
AU  - Jin M
AD  - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary 
      Medicine, Jilin University, Changchun 130062, PR China.
FAU - Zheng, Lianwen
AU  - Zheng L
AD  - Reproductive Medical Center, the Second Hospital of Jilin University, Changchun, 
      PR China.
FAU - Wei, Yunfei
AU  - Wei Y
AD  - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary 
      Medicine, Jilin University, Changchun 130062, PR China.
FAU - Cheng, Jiaqi
AU  - Cheng J
AD  - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary 
      Medicine, Jilin University, Changchun 130062, PR China.
FAU - Zhang, Di
AU  - Zhang D
AD  - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary 
      Medicine, Jilin University, Changchun 130062, PR China.
FAU - Yan, Siru
AU  - Yan S
AD  - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary 
      Medicine, Jilin University, Changchun 130062, PR China.
FAU - Qin, Haiyan
AU  - Qin H
AD  - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary 
      Medicine, Jilin University, Changchun 130062, PR China.
FAU - Wang, Qi
AU  - Wang Q
AD  - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary 
      Medicine, Jilin University, Changchun 130062, PR China.
FAU - Ci, Xinxin
AU  - Ci X
AD  - Institute of Translational Medicine, The First Hospital, Jilin University, 
      Changchun 130001, PR China.
FAU - Feng, Haihua
AU  - Feng H
AD  - Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary 
      Medicine, Jilin University, Changchun 130062, PR China. Electronic address: 
      fhh70@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220122
PL  - Netherlands
TA  - Environ Toxicol Pharmacol
JT  - Environmental toxicology and pharmacology
JID - 9612020
RN  - EC 2.6.1.1 (Aspartate Aminotransferases)
SB  - IM
MH  - Animals
MH  - Aorta/pathology
MH  - Aspartate Aminotransferases/blood
MH  - Atherosclerosis/*etiology
MH  - Enterobacter cloacae/pathogenicity
MH  - Enterobacteriaceae Infections/*pathology
MH  - Guinea Pigs
MH  - Inflammation
MH  - Lipid Metabolism
MH  - Liver/metabolism/*pathology
MH  - Male
MH  - Non-alcoholic Fatty Liver Disease/*etiology
OTO - NOTNLM
OT  - Atherosclerosis
OT  - E. cloacae
OT  - Inflammation
OT  - Lipid accumulation
OT  - NAFLD
EDAT- 2022/01/26 06:00
MHDA- 2022/02/24 06:00
CRDT- 2022/01/25 20:12
PHST- 2021/08/23 00:00 [received]
PHST- 2022/01/03 00:00 [revised]
PHST- 2022/01/20 00:00 [accepted]
PHST- 2022/01/26 06:00 [pubmed]
PHST- 2022/02/24 06:00 [medline]
PHST- 2022/01/25 20:12 [entrez]
AID - S1382-6689(22)00012-6 [pii]
AID - 10.1016/j.etap.2022.103819 [doi]
PST - ppublish
SO  - Environ Toxicol Pharmacol. 2022 Feb;90:103819. doi: 10.1016/j.etap.2022.103819. 
      Epub 2022 Jan 22.