PMID- 35080160
OWN - NLM
STAT- MEDLINE
DCOM- 20220127
LR  - 20220915
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 40
IP  - 2
DP  - 2022 Feb 8
TI  - [Dispersive solid-phase extraction combined with high-performance liquid 
      chromatography for determination of seven anesthetics in aquatic products].
PG  - 139-147
LID - 10.3724/SP.J.1123.2021.08002 [doi]
AB  - Nowadays, anesthetics are widely used in fishery production processes, such as 
      fish breeding, surgery, and fresh aquatic product transportation. Because of the 
      widespread application of anesthetic drugs in aquatic products, there is an 
      increasing demand for the rapid and sensitive detection of anesthetic drugs in 
      aquatic products. The complex aquatic product matrix contains a variety of 
      interfering substances, such as proteins, fats, and phospholipids, along with 
      anesthetic drug residues at very low concentrations; therefore, it is necessary 
      to adopt appropriate pretreatment methods for improving the sensitivity of 
      detection. In this study, a dispersive solid-phase extraction (DSPE) method, 
      combined with high-performance liquid chromatography, was established for the 
      simultaneous detection of seven anesthetic drugs in aquatic products, viz. 
      procaine, oxybuprocaine, tricaine, eugenol, methyl eugenol, isoeugenol, and 
      methyl isoeugenol. For the DSPE step, pretreatment conditions, such as extraction 
      solvent, extraction time, adsorbent amount, and DMSO dosage, were optimized. 
      Sample pretreatment is a three-step process. First, in ultrasound-assisted 
      extraction, 2.0 g samples were extracted using 10.0 mL 1.0% formic acid in 
      acetonitrile under ultrasound conditions for 10 min. Then, DSPE was performed 
      with mixed adsorbents: the solvent extracts were cleaned using 20 mg 
      poly(styrene-glycidylmethacrylate) microspheres (PS-GMA), 50 mg primary secondary 
      amines (PSA), and 10 mg C18, followed by separation by centrifugation. Finally, 
      DMSO-assisted concentration was applied: the organic layer was collected and was 
      dried at 40 ℃ in a N(2) stream with 100 muL DMSO. Water was added to the residue 
      to obtain a final volume of 1.0 mL for HPLC analysis. The seven anesthetic drugs 
      were separated on a Welch welchrom C18 column (250 mmx4.6 mm, 5 mum) by gradient 
      elution using methanol and 0.05% formic acid in 5 mmol/L ammonium acetate aqueous 
      solution as mobile phases. The detection wavelengths were 235, 260, and 290 nm. 
      Two matrix matching standard curves for fish and shrimp were applied for 
      quantitative analysis. Under optimized conditions, the seven target anesthetics 
      showed good linear relationships in their respective concentration ranges 
      (R(2)>0.999), with the limit of detection (LOD) ranging from 0.011 to 0.043 
      mg/kg. In fish samples, the mean recoveries obtained at three concentration 
      levels were between 79.7% and 109%, with relative standard deviations (RSDs) 
      being less than 7.2%. In shrimp samples, mean recoveries were 78.0%-99.9%, with 
      RSDs being less than 8.3%. This simple, rapid, accurate, and sensitive method can 
      be applied to the detection of three kinds of aminobenzoic acid esters and four 
      kinds of eugenol anesthetic drugs in aquatic products.
FAU - Shi, Fang
AU  - Shi F
AD  - Department of Chemistry, Zhejiang University, Hangzhou 310028, China.
FAU - Shou, Dan
AU  - Shou D
AD  - Department of Medicine, Zhejiang Academy of Traditional Chinese Medicine, 
      Hangzhou 310007, China.
FAU - Jin, Micong
AU  - Jin M
AD  - Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, 
      China;6. Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of 
      Zhejiang Province, Zhejiang University, Hangzhou 310028, China.
FAU - Wang, Hongwei
AU  - Wang H
AD  - Tongde Hospital of Zhejiang Province, Hangzhou 310012, China.
FAU - Chen, Xuguang
AU  - Chen X
AD  - Zhejiang Metallurgical Research Institute Co., LTD., Hangzhou 310007, China.
FAU - Zhu, Yan
AU  - Zhu Y
AD  - Department of Chemistry, Zhejiang University, Hangzhou 310028, China;6. Key 
      Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang 
      Province, Zhejiang University, Hangzhou 310028, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Anesthetics)
SB  - IM
MH  - *Anesthetics
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - *Drug Residues/analysis
MH  - Solid Phase Extraction
MH  - Tandem Mass Spectrometry
PMC - PMC9404131
OTO - NOTNLM
OT  - anesthetic drugs
OT  - aquatic products
OT  - dispersive solid-phase extraction (DSPE)
OT  - high-performance liquid chromatography (HPLC)
EDAT- 2022/01/27 06:00
MHDA- 2022/01/28 06:00
PMCR- 2022/02/08
CRDT- 2022/01/26 05:34
PHST- 2022/01/26 05:34 [entrez]
PHST- 2022/01/27 06:00 [pubmed]
PHST- 2022/01/28 06:00 [medline]
PHST- 2022/02/08 00:00 [pmc-release]
AID - 1000-8713-40-2-139 [pii]
AID - 10.3724/SP.J.1123.2021.08002 [doi]
PST - ppublish
SO  - Se Pu. 2022 Feb 8;40(2):139-147. doi: 10.3724/SP.J.1123.2021.08002.