PMID- 35080161
OWN - NLM
STAT- MEDLINE
DCOM- 20220127
LR  - 20220915
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 40
IP  - 2
DP  - 2022 Feb 8
TI  - [Determination of nine food-borne stimulant drug residues in pork, egg, and milk 
      by ultra-performance liquid chromatography-tandem mass spectrometry].
PG  - 148-155
LID - 10.3724/SP.J.1123.2021.04005 [doi]
AB  - beta-Agonists, beta-blockers, and protein assimilators are classified as stimulant 
      drugs. Their illegal use during animal feeding and slaughtering leads to 
      food-borne stimulant drug residues, which are harmful to human health. At 
      present, methods for the detection of beta-agonists and protein assimilators are 
      prevalent, but those for the detection of beta-blockers are rare. There is no 
      national standard for the detection of beta-blockers in food products of animal 
      origin. A method based on ultra-performance liquid chromatography-tandem mass 
      spectrometry (UPLC-MS/MS) was developed for the determination of nine food-borne 
      stimulant drug residues, including beta-agonists, beta-blockers, and protein 
      assimilators, in pork, egg, and milk. The optimal extraction conditions for this 
      method were as follows: The samples were hydrolyzed with beta-glucuronidase/aryl 
      sulfate esterase in pH 5.2 ammonium acetate buffer. Enzymatic hydrolysis was 
      carried out in a constant-temperature (37 ℃) water bath oscillator for 16 h. The 
      enzymolyzed samples were cooled to room temperature and then extracted with 
      acetonitrile, which was adjusted to pH 9.5 with NaOH solution. After extraction 
      and homogeneous mixing, the extract was added to a salt package for salting out 
      stratification. The clear supernatant was cleaned up using an enhanced lipid 
      removal tube (EMR-lipid), which was pre-activated by water. Then, anhydrous 
      magnesium sulfate was added to ensure dehydration of the extract and concentrated 
      to near dryness under nitrogen flow. The residue was dissolved in 1 mL 
      acetonitrile-0.1% formic acid aqueous solution (1ratio9, v/v). Separation was 
      performed on an ACQUITY UPLC HSS T3 column (100 mmx2.1 mm, 1.8 mum) with gradient 
      elution using methanol-0.1% formic acid aqueous solution as the mobile phase. The 
      analytes were detected in the multiple reaction monitoring (MRM) mode after being 
      ionized by an electrospray positive ion (ESI(+)). Quantitative analysis was 
      performed by the internal standard method using matrix-matched calibration 
      curves. The effects of the extraction solvent and pH on the extraction efficiency 
      during pretreatment were optimized. The influence factors of different types of 
      chromatographic column, mobile phase and dissolved solution in the process of 
      instrumental analysis were discussed in detail. Under the optimal conditions, the 
      method showed good linearity in the range of 0.5 to 20 mug/L, with correlation 
      coefficients (r(2)) greater than 0.99. The limits of detection (LODs) and limits 
      of quantification (LOQs) were in the range of 0.3-0.6 mug/kg and 1.0-2.0 mug/kg, 
      respectively. The average recoveries of all the compounds ranged from 65.2% to 
      117.0% with relative standard deviations (RSDs) in range of 1.3%-14.4% at spiked 
      levels of 1, 2, and 5 times the LOQs. The established method was used to 
      determine the quality of animal-origin foods such as pork, eggs, and milk 
      purchased from the market. The nine stimulant drug residues were not detected in 
      these food samples. The analytical method is rapid, sensitive, accurate, and 
      stable. It can be used for the determination of the nine food-borne stimulant 
      drugs residue in pork, egg, and milk.
FAU - Liu, Xuezhi
AU  - Liu X
AD  - Chinese Academy of Inspection and Quarantine Comprehensive Test Center, Beijing 
      100123, China.
FAU - Zhao, Yinglian
AU  - Zhao Y
AD  - Chinese Academy of Inspection and Quarantine Comprehensive Test Center, Beijing 
      100123, China.
FAU - Ma, Yue
AU  - Ma Y
AD  - China Inspection Branch (Beijing) Test Technology Co., Ltd., Beijing 100176, 
      China.
FAU - Dong, Shishi
AU  - Dong S
AD  - Chinese Academy of Inspection and Quarantine Comprehensive Test Center, Beijing 
      100123, China.
FAU - Wang, Bin
AU  - Wang B
AD  - Chinese Academy of Inspection and Quarantine Comprehensive Test Center, Beijing 
      100123, China.
FAU - Zhang, Yang
AU  - Zhang Y
AD  - China Inspection Branch (Beijing) Test Technology Co., Ltd., Beijing 100176, 
      China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Liquid
MH  - *Drug Residues/analysis
MH  - Food Contamination/analysis
MH  - Humans
MH  - Milk
MH  - *Pork Meat
MH  - *Red Meat
MH  - Solid Phase Extraction
MH  - Swine
MH  - Tandem Mass Spectrometry
PMC - PMC9404034
OTO - NOTNLM
OT  - egg
OT  - food-borne stimulant drug
OT  - milk
OT  - pork
OT  - residues
OT  - ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)
EDAT- 2022/01/27 06:00
MHDA- 2022/01/28 06:00
PMCR- 2022/02/08
CRDT- 2022/01/26 05:34
PHST- 2022/01/26 05:34 [entrez]
PHST- 2022/01/27 06:00 [pubmed]
PHST- 2022/01/28 06:00 [medline]
PHST- 2022/02/08 00:00 [pmc-release]
AID - 1000-8713-40-2-148 [pii]
AID - 10.3724/SP.J.1123.2021.04005 [doi]
PST - ppublish
SO  - Se Pu. 2022 Feb 8;40(2):148-155. doi: 10.3724/SP.J.1123.2021.04005.