PMID- 35080701
OWN - NLM
STAT- MEDLINE
DCOM- 20220426
LR  - 20220426
IS  - 1708-0428 (Electronic)
IS  - 0960-8923 (Linking)
VI  - 32
IP  - 4
DP  - 2022 Apr
TI  - Role of Proximal Intestinal Glucose Sensing and Metabolism in the Blood Glucose 
      Control in Type 2 Diabetic Rats After Duodenal Jejunal Bypass Surgery.
PG  - 1119-1129
LID - 10.1007/s11695-021-05871-3 [doi]
AB  - BACKGROUND: Although gastric surgery can significantly improve blood glucose 
      homeostasis in type 2 diabetes mellitus (T2DM), its mechanism remains unclear. 
      This study evaluated the role of intestinal glucose sensing, glucose transport, 
      and metabolism in the alimentary limb (A limb) of T2DM rats after duodenal 
      jejunal bypass (DJB) surgery. METHODS: A T2DM rat model was induced via a 
      high-glucose high-fat diet and low-dose streptozotocin injection. The diabetic 
      rats were divided into two groups: the DJB surgery (T2DM-DJB) group and the sham 
      surgery (T2DM-Sham) group. Wistar rats were used as wild-type control (Control). 
      Small animal PET was used to assess the change in glucose metabolic status in the 
      intestine. The intestinal villi height and the number of EECs after DJB were 
      evaluated. The expressions of sweet taste receptors (T1R2/T1R3), glucose 
      transporters (SGLT1/GLUT2), and key enzymes involved in glucose metabolism (HK2, 
      PFK2, PKM2, G6Pase, and PCK1) in the A limb after DJB was detected by Western 
      blot and qRT-PCR. RESULTS: Small animal PET analysis showed the intestinal 
      glucose metabolism increased significantly 6 weeks after DJB surgery. The 
      intestinal villi height and the number of EECs in the A limb 6 weeks after 
      surgery increased significantly in T2DM-DJB rats comparing to T2DM-Sham rats. The 
      mRNA and protein expression of T1R1/T1R3 and SGLT1/GLUT2 were downregulated in 
      DJB-T2DM rats, while enzymes involved in glucose metabolism was upregulated in 
      the A limb in T2DM-DJB rats. CONCLUSION: Proximal intestinal glucose sensing and 
      metabolism play an important role in blood glucose homeostasis by DJB.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Jiang, Bin
AU  - Jiang B
AD  - Translational Medical Center, Weifang Second People's Hospital, Weifang, 261041, 
      China.
AD  - School of Life Science and Technology, Weifang Medical University, Weifang, 
      261053, China.
FAU - Wang, Huaijie
AU  - Wang H
AD  - Translational Medical Center, Weifang Second People's Hospital, Weifang, 261041, 
      China.
FAU - Li, Na
AU  - Li N
AD  - Translational Medical Center, Weifang Second People's Hospital, Weifang, 261041, 
      China.
FAU - Yan, Qingtao
AU  - Yan Q
AD  - Department of Pediatric Surgery, Weifang People's Hospital, The First Affiliated 
      Hospital of Weifang Medical University, Weifang, 261041, China.
FAU - Wang, Weiyu
AU  - Wang W
AD  - Translational Medical Center, Weifang Second People's Hospital, Weifang, 261041, 
      China.
FAU - Wang, Yubing
AU  - Wang Y
AD  - School of Life Science and Technology, Weifang Medical University, Weifang, 
      261053, China.
FAU - Xue, Hantao
AU  - Xue H
AD  - School of Life Science and Technology, Weifang Medical University, Weifang, 
      261053, China.
FAU - Ma, Shengyao
AU  - Ma S
AD  - Translational Medical Center, Weifang Second People's Hospital, Weifang, 261041, 
      China.
AD  - School of Pharmacy, Weifang Medical University, Weifang, 261053, China.
FAU - Li, Xiaocheng
AU  - Li X
AD  - School of Life Science and Technology, Weifang Medical University, Weifang, 
      261053, China.
FAU - Diao, Wenbin
AU  - Diao W
AD  - School of Life Science and Technology, Weifang Medical University, Weifang, 
      261053, China.
FAU - Pan, Ruiyan
AU  - Pan R
AD  - School of Pharmacy, Weifang Medical University, Weifang, 261053, China. 
      panruiyan2011@126.com.
FAU - Gao, Zhiqin
AU  - Gao Z
AD  - School of Life Science and Technology, Weifang Medical University, Weifang, 
      261053, China. zhiqingao2013@163.com.
FAU - Qu, Mei-Hua
AU  - Qu MH
AUID- ORCID: 0000-0003-2464-6773
AD  - Translational Medical Center, Weifang Second People's Hospital, Weifang, 261041, 
      China. qumeihua2016@163.com.
AD  - School of Life Science and Technology, Weifang Medical University, Weifang, 
      261053, China. qumeihua2016@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220126
PL  - United States
TA  - Obes Surg
JT  - Obesity surgery
JID - 9106714
RN  - 0 (Blood Glucose)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - *Diabetes Mellitus, Experimental/metabolism/surgery
MH  - *Diabetes Mellitus, Type 2/metabolism/surgery
MH  - Duodenum/metabolism/surgery
MH  - *Gastric Bypass
MH  - Glucose/metabolism
MH  - Glycemic Control
MH  - Humans
MH  - Jejunum/metabolism/surgery
MH  - *Obesity, Morbid/surgery
MH  - Rats
MH  - Rats, Wistar
OTO - NOTNLM
OT  - DJB surgery
OT  - Glucose homeostasis
OT  - Sweet receptors
OT  - Type 2 diabetes mellitus
EDAT- 2022/01/27 06:00
MHDA- 2022/04/27 06:00
CRDT- 2022/01/26 12:12
PHST- 2021/05/17 00:00 [received]
PHST- 2021/12/29 00:00 [accepted]
PHST- 2021/12/27 00:00 [revised]
PHST- 2022/01/27 06:00 [pubmed]
PHST- 2022/04/27 06:00 [medline]
PHST- 2022/01/26 12:12 [entrez]
AID - 10.1007/s11695-021-05871-3 [pii]
AID - 10.1007/s11695-021-05871-3 [doi]
PST - ppublish
SO  - Obes Surg. 2022 Apr;32(4):1119-1129. doi: 10.1007/s11695-021-05871-3. Epub 2022 
      Jan 26.